Invitrogen

CD106 (VCAM-1) Monoclonal Antibody (STA), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10 Published Figures

15 References

View all (94) VCAM-1 (CD106) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD106 (VCAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 12

CD106 (VCAM-1) Antibody (12-1069-42) in Flow

HUVEC were stimulated for 16 hours with Human TNF alpha Recombinant Protein (Product # 14-8329-81) and Human IFN alpha 2 Recombinant Protein, then stained with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD106 (VCAM-1) PE (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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Product Details

12-1069-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

STA

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854126

Product Specific Information

Description: The STA monoclonal antibody reacts with human CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to Integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1). Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: STA has been reported for use in flow cytometric analysis.

Applications Tested: This STA antibody has been pre-titrated and tested by flow cytometric analysis of stimulated HUVEC. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis.

Food science & nutrition

Festa J,Hussain A,Hackney A,Desai U,Sahota TS,Singh H,Da Boit M

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 12-1069-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Multimodal CRISPR perturbations of GWAS loci associated with coronary artery disease in vascular endothelial cells.

PLoS genetics

Wünnemann F,Fotsing Tadjo T,Beaudoin M,Lalonde S,Lo KS,Kleinstiver BP,Lettre G

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 12-1069-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils.

Nature communications

Manriquez V,Nivoit P,Urbina T,Echenique-Rivera H,Melican K,Fernandez-Gerlinger MP,Flamant P,Schmitt T,Bruneval P,Obino D,Duménil G

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 12-1069-42) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Differential Characterization of Temozolomide-Resistant Human Glioma Cells.

International journal of molecular sciences

Lai SW,Huang BR,Liu YS,Lin HY,Chen CC,Tsai CF,Lu DY,Lin C

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 12-1069-42) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

Expansion of functional personalized cells with specific transgene combinations.

Nature communications

Lipps C,Klein F,Wahlicht T,Seiffert V,Butueva M,Zauers J,Truschel T,Luckner M,Köster M,MacLeod R,Pezoldt J,Hühn J,Yuan Q,Müller PP,Kempf H,Zweigerdt R,Dittrich-Breiholz O,Pufe T,Beckmann R,Drescher W,Riancho J,Sañudo C,Korff T,Opalka B,Rebmann V,Göthert JR,Alves PM,Ott M,Schucht R,Hauser H,Wirth D,May T

12-1069 was used in Flow cytometry/Cell sorting to develop a novel strategy for rapid, functional, personalised cell expansion.

Thu Mar 08 00:00:00 EST 2018

Distinctive expression of T cell guiding molecules in human autoimmune lymph node stromal cells upon TLR3 triggering.

Scientific reports

Hähnlein JS,Ramwadhdoebe TH,Semmelink JF,Choi IY,Berger FH,Maas M,Gerlag DM,Tak PP,Geijtenbeek TBH,van Baarsen LGM

12-1069 was used in Flow cytometry/Cell sorting to investigate the ability of human LNSCs derived from RA, RA-risk and healthy individuals to sense and respond to pathogens.

Mon Jan 29 00:00:00 EST 2018

A high-yield isolation and enrichment strategy for human lung microvascular endothelial cells.

Pulmonary circulation

Gaskill C,Majka SM

12-1069 was used in Flow cytometry/Cell sorting to describe a protocol to select large quantities of enriched primary human lung microvascular endothelial cells, free from mesenchymal contamination.

Wed Mar 01 00:00:00 EST 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 12-1069-42) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

TNF-α enhances vascular cell adhesion molecule-1 expression in human bone marrow mesenchymal stem cells via the NF-κB, ERK and JNK signaling pathways.

Molecular medicine reports

Lu ZY,Chen WC,Li YH,Li L,Zhang H,Pang Y,Xiao ZF,Xiao HW,Xiao Y

Fri Jul 01 00:00:00 EDT 2016

Acidic pH reduces VEGF-mediated endothelial cell responses by downregulation of VEGFR-2; relevance for anti-angiogenic therapies.

Oncotarget

Faes S,Uldry E,Planche A,Santoro T,Pythoud C,Demartines N,Dormond O

12-1069 was used in Flow cytometry/Cell sorting to show that endothelial cells prosper independently of VEGF in acidic conditions partly as a consequence of decreased VEGFR-2 expression.

Tue Dec 27 00:00:00 EST 2016

Cytokine induction of VCAM-1 but not IL13Rα2 on glioma cells: a tale of two antibodies.

PloS one

Mahadev V,Starr R,Wright SL,Martinez C,Jensen MC,Barish ME,Forman SJ,Brown CE

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 12-1069-42) in Flow Cytometry

Mon Dec 22 00:00:00 EST 2014

Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.

PloS one

An N,Andrukhov O,Tang Y,Falkensammer F,Bantleon HP,Ouyang X,Rausch-Fan X

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 12-1069-42) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2015

Nitric oxide inhibits hetero-adhesion of cancer cells to endothelial cells: restraining circulating tumor cells from initiating metastatic cascade.

Scientific reports

Lu Y,Yu T,Liang H,Wang J,Xie J,Shao J,Gao Y,Yu S,Chen S,Wang L,Jia L

12-1069 was used in Flow cytometry/Cell sorting to highlight the importance of NO in cancer metastatic prevention.

Tue Mar 11 00:00:00 EDT 2014

Effects of Aggregatibacter actinomycetemcomitans leukotoxin on endothelial cells.

Microbial pathogenesis

Dietmann A,Millonig A,Combes V,Couraud PO,Kachlany SC,Grau GE

12-1069 was used in Flow cytometry/Cell sorting to demonstrate that Aggregatibacter actinomycetemcomitans leukotoxin has substantial pro-inflammatory effects on human brain endothelial cells by upregulation of ICAM-1 and VCAM-1.

Wed Feb 12 00:00:00 EST 2014

Effects of Aggregatibacter actinomycetemcomitans leukotoxin on endothelial cells.

Microbial pathogenesis

Dietmann A,Millonig A,Combes V,Couraud PO,Kachlany SC,Grau GE

Wed Feb 12 00:00:00 EST 2014

Bioinformatics

Protein Aliases: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1

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Gene Aliases: CD106; INCAM-100; VCAM1

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UniProt ID: (Human) P19320

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Entrez Gene ID: (Human) 7412

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Function(s)

integrin binding primary amine oxidase activity cell adhesion molecule binding

Process(es)

response to hypoxia acute inflammatory response chronic inflammatory response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion aging response to nutrient amine metabolic process response to zinc ion response to ionizing radiation viral process membrane to membrane docking B cell differentiation extracellular matrix organization response to lipopolysaccharide response to nicotine calcium-mediated signaling using intracellular calcium source cellular response to vascular endothelial growth factor stimulus positive regulation of T cell proliferation response to ethanol regulation of immune response leukocyte tethering or rolling oxidation-reduction process cell chemotaxis interferon-gamma-mediated signaling pathway cellular response to tumor necrosis factor

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD106 (VCAM-1) Monoclonal Antibody (STA), PE, eBioscience™

CD106 (VCAM-1) Antibody (12-1069-42) in Flow

Product Details

12-1069-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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