Invitrogen

CD106 (VCAM-1) Monoclonal Antibody (eBioMR106 (MR106)), Biotin, eBioscience™

CD106 (VCAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD106 (VCAM-1) Antibody (13-1060-82) in Flow

Staining of rat bone marrow cells with 0.125 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (left) or 0.125 µg of Anti-Rat CD106 (VCAM-1) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD106 (VCAM-1) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

13-1060-82

Applications

Tested Dilution

Publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test

View 2 publications 2 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioMR106 (MR106)

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_529468

Product Specific Information

Description: The eBioMR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: This eBioMR106 (MR106) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioMR106 (MR106) antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Blood-brain barrier dysfunction in L-ornithine induced acute pancreatitis in rats and the direct effect of L-ornithine on cultured brain endothelial cells.

Fluids and barriers of the CNS

Walter FR,Harazin A,Tóth AE,Veszelka S,Santa-Maria AR,Barna L,Kincses A,Biczó G,Balla Z,Kui B,Maléth J,Cervenak L,Tubak V,Kittel Á,Rakonczay Z,Deli MA

13-1060-82 was used in Immunohistochemistry (PFA fixed) to suggest a direct effect of the cationic L-ornithine on brain endothelium. Endothelial surface glycocalyx injury was revealed both in vivo and in vitro, as an additional novel component of the BBB-related pathological changes in AP.

Thu Feb 17 00:00:00 EST 2022

Blood-brain barrier dysfunction in L-ornithine induced acute pancreatitis in rats and the direct effect of L-ornithine on cultured brain endothelial cells.

Fluids and barriers of the CNS

Walter FR,Harazin A,Tóth AE,Veszelka S,Santa-Maria AR,Barna L,Kincses A,Biczó G,Balla Z,Kui B,Maléth J,Cervenak L,Tubak V,Kittel Á,Rakonczay Z,Deli MA

Thu Feb 17 00:00:00 EST 2022

Early Proteome Shift and Serum Bioactivity Precede Diesel Exhaust-induced Impairment of Cardiovascular Recovery in Spontaneously Hypertensive Rats.

Scientific reports

Thompson LC,Shannahan JH,Perez CM,Haykal-Coates N,King C,Hazari MS,Brown JM,Farraj AK

13-1060-82 was used in Flow Cytometry to determine if exposure-induced cardiovascular dysfunction can be coupled with alterations in both serum bioactivity and the circulating proteome.

Fri May 03 00:00:00 EDT 2019

Identification of New Rat Bone Marrow-Derived Population of Very Small Stem Cell with Oct-4A and Nanog Expression by Flow Cytometric Platforms.

Stem cells international

Labedz-Maslowska A,Kamycka E,Bobis-Wozowicz S,Madeja Z,Zuba-Surma EK

13-1060 was used in Flow cytometry/Cell sorting to identify multiantigenic phenotype and providing sorting protocols for isolation VSELs from rat BM tissue for further examining of their functional properties.

Fri Dec 04 00:00:00 EST 2015

From the Cover: Disease-Induced Disparities in Formation of the Nanoparticle-Biocorona and the Toxicological Consequences.

Toxicological sciences : an official journal of the Society of Toxicology

Shannahan JH,Fritz KS,Raghavendra AJ,Podila R,Persaud I,Brown JM

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1060-82) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2016

From the Cover: Disease-Induced Disparities in Formation of the Nanoparticle-Biocorona and the Toxicological Consequences.

Toxicological sciences : an official journal of the Society of Toxicology

Shannahan JH,Fritz KS,Raghavendra AJ,Podila R,Persaud I,Brown JM

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 13-1060-82) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2016

Temporal pattern of ICAM-I mediated regulatory T cell recruitment to sites of inflammation in adoptive transfer model of multiple sclerosis.

PloS one

Doerck S,Göbel K,Weise G,Schneider-Hohendorf T,Reinhardt M,Hauff P,Schwab N,Linker R,Mäurer M,Meuth SG,Wiendl H

13-1060 was used in Functional assays to explore the migration of pro-inflammatory and regulatory T cells towards target organs in an in vivo model of multiple sclerosis.

Mon Nov 15 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD106; DKFZp779G2333; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1; VCAM-1

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Gene Aliases: Vcam-1; Vcam1; VCAM1B

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UniProt ID: (Rat) P29534

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Entrez Gene ID: (Rat) 25361

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Function(s)

integrin binding primary amine oxidase activity cell adhesion molecule binding

Process(es)

response to hypoxia acute inflammatory response chronic inflammatory response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion heart development aging response to nutrient amine metabolic process response to zinc ion response to ionizing radiation viral process membrane to membrane docking response to lipopolysaccharide response to nicotine cellular response to vascular endothelial growth factor stimulus positive regulation of T cell proliferation response to ethanol leukocyte tethering or rolling oxidation-reduction process cell chemotaxis embryonic placenta morphogenesis chorio-allantoic fusion cellular response to glucose stimulus cellular response to tumor necrosis factor

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD106 (VCAM-1) Monoclonal Antibody (eBioMR106 (MR106)), Biotin, eBioscience™

CD106 (VCAM-1) Antibody (13-1060-82) in Flow

Product Details

13-1060-82

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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