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Invitrogen
CD109 Monoclonal Antibody (HU17), eBioscience™
FIGURE: 1 / 4
CD109 Antibody (14-1099-80) in Flow
Staining of unstimulated (left) or 3-day PHA-stimulated (right) normal human peripheral blood cells with 0.125 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.125 µg of Anti-Human CD109 Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Total viable cells were used for analysis.
Product Details
14-1099-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HU17
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_842737
Product Specific Information
Description: The HU17 monoclonal antibody reacts with human CD109, also known as the platelet-specific Gov antigen. CD109, a GPI-anchored monomeric glycoprotein, exists in two isoforms of 120 and 150 kDa, which are proteolytically degraded from a 170 kDa form. CD109 is expressed on activated T cells, activated platelets and endothelial cells. CD109 expression is also found on a subset of CD34+ cells in adult and fetal bone marrow which contain megakaryocyte, myeloid, erythroid and multilineage progenitors, as well as pluriopotent hematopoietic stem cells. Recently, it has been demonstrated that CD109 is expressed on a variety of human cancer cell lines, and also lung and esophogeal cell carcinomas. In addition, CD109 was recently found to be a negative regulator of TGF-beta in keratinocytes.
Applications Reported: This HU17 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.
Applications Tested: This HU17 antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD109 is a member of the alpha2-macroglobulin/complement superfamily. The encoded GPI-linked glycoprotein is found on the cell surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 150 kDa TGF-beta-1-binding protein; activated T-cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD109; CD109 antigen; Gov platelet alloantigens; p180; Platelet-specific Gov antigen; r150; RP11-525G3.1
Gene Aliases: CD109; CPAMD7; p180; r150
UniProt ID: (Human) Q6YHK3
Entrez Gene ID: (Human) 135228
negative regulation of protein phosphorylation
hair follicle development
negative regulation of keratinocyte proliferation
negative regulation of endopeptidase activity
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of keratinocyte differentiation
negative regulation of wound healing
osteoclast fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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