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Invitrogen
CD116 Monoclonal Antibody (4H1), eBioscience™
Product Details
14-1169-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
4H1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_467431
Product Specific Information
Description: The 4H1 monoclonal antibody reacts with the human CD116 molecule, the alpha subunit of GM-CSF receptor. The alpha subunit associates with the common beta chain (CD131) to form the high affinity receptor for GM-CSF. The GM-CSFR alpha chain is expressed by granulocytes, monocytes, endothelial cells, fibroblasts and some tumor cells.
Applications Reported: 4H1 has been reported for use in flow cytometric analysis.
Applications Tested: The 4H1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD116; CD116 antigen; CDw116; colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage); CSF 2R; GM CSF R alpha; GM-CSF receptor alpha subunit; GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; granulocyte-macrophage colony-stimulating factor receptor alpha chain; Granulocyte-macrophage colony-stimulating factor receptor subunit alpha; MGC3848; MGC4838
Gene Aliases: CD116; CDw116; CSF2R; CSF2RA; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; GM-CSF-R-alpha; GMCSFR; GMR; SMDP4
UniProt ID: (Human) P15509
Entrez Gene ID: (Human) 1438
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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