Invitrogen

CD117 (c-Kit) Monoclonal Antibody (YB5.B8), eBioscience™

13 Published Figures
7 References
View all (212) c-Kit (CD117) antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

14-1179-82

Applications
Tested Dilution
Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent
-

Flow Cytometry (Flow)

1 µg/test
View 7 publications 7 publications

Immunoprecipitation (IP)

Assay-Dependent
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

YB5.B8

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467439

Product Specific Information

Description: The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells.

Applications Reported: YB5.B8 has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections.

Applications Tested: The YB5.B8 antibody has been tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Gene Aliases: C-Kit; CD117; KIT; PBT; SCFR

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UniProt ID: (Human) P10721

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Entrez Gene ID: (Human) 3815

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Function(s)
protease binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding cytokine binding protein homodimerization activity metal ion binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity transmembrane signal receptor
Process(es)
MAPK cascade activation of MAPK activity ovarian follicle development myeloid progenitor cell differentiation lymphoid progenitor cell differentiation immature B cell differentiation dendritic cell cytokine production mast cell chemotaxis glycosphingolipid metabolic process inflammatory response signal transduction spermatogenesis spermatid development positive regulation of cell proliferation germ cell migration regulation of cell shape visual learning male gonad development positive regulation of gene expression positive regulation of phospholipase C activity regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway stem cell population maintenance lamellipodium assembly hemopoiesis T cell differentiation erythrocyte differentiation melanocyte differentiation positive regulation of cell migration positive regulation of pseudopodium assembly actin cytoskeleton reorganization mast cell cytokine production somatic stem cell population maintenance embryonic hemopoiesis ectopic germ cell programmed cell death hematopoietic stem cell migration megakaryocyte development Fc receptor signaling pathway Kit signaling pathway erythropoietin-mediated signaling pathway regulation of cell proliferation positive regulation of tyrosine phosphorylation of Stat1 protein positive regulation of tyrosine phosphorylation of Stat3 protein positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of programmed cell death mast cell degranulation positive regulation of MAPK cascade pigmentation positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity positive regulation of Notch signaling pathway positive regulation of JAK-STAT cascade protein autophosphorylation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of developmental pigmentation somatic stem cell division positive regulation of long-term neuronal synaptic plasticity digestive tract development stem cell differentiation epithelial cell proliferation detection of mechanical stimulus involved in sensory perception of sound positive regulation of sequence-specific DNA binding transcription factor activity cell chemotaxis mast cell differentiation mast cell proliferation cellular response to thyroid hormone stimulus melanocyte migration melanocyte adhesion positive regulation of vascular smooth muscle cell differentiation
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