Invitrogen

CD119 (IFN gamma Receptor 1) Monoclonal Antibody (2E2), PE, eBioscience™

4 Published Figures

13 References

View all (33) IFNGR1 (CD119) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

CD119 (IFN gamma Receptor 1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD119 (IFN gamma Receptor 1) Antibody (12-1191-82) in Flow

Staining of BALB/c splenocytes with 1 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (blue histogram) or 1 µg of Anti-Mouse CD119 (IFN gamma Receptor 1) PE (purple histogram). Total viable cells were used for analysis.

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Product Details

12-1191-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2E2

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210730

Product Specific Information

Description: The monoclonal antibody 2E2 recognizes mouse CD119 also known as IFN gamma receptor1. CD119 heterodimerizes with IFN gamma receptor 2 (AF-1) forming the Class II cytokine receptor. The ligand IFN gamma binds IFN gamma receptor 1 subunit but signal transduction transpires via the IFN gamma receptor 2 subunit. The receptor is involved in host defense and expression is broad including B cells, NK cells, macrophages and dendritic cells as well as other non-hematopoietic cell types.

Applications Reported: This 2E2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2E2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, longer incubations appear to help stain the popµLation; therefore we recommend a 60 minute incubation at 2-8°C.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Senescence Rewires Microenvironment Sensing to Facilitate Antitumor Immunity.

Cancer discovery

Chen HA,Ho YJ,Mezzadra R,Adrover JM,Smolkin R,Zhu C,Woess K,Bernstein N,Schmitt G,Fong L,Luan W,Wuest A,Tian S,Li X,Broderick C,Hendrickson RC,Egeblad M,Chen Z,Alonso-Curbelo D,Lowe SW

Published figure using CD119 (IFN gamma Receptor 1) monoclonal antibody (Product # 12-1191-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2023

STUB1 is an intracellular checkpoint for interferon gamma sensing.

Scientific reports

Ng S,Lim S,Sim ACN,Mangadu R,Lau A,Zhang C,Martinez SB,Chandramohan A,Lim UM,Ho SSW,Chang SC,Gopal P,Hong LZ,Schwaid A,Fernandis AZ,Loboda A,Li C,Phan U,Henry B,Partridge AW

12-1191-82 was used in Flow cytometry/Cell sorting to elucidate STUB1 as a barrier to IFNγ sensing, prompting further investigations to assess if broader inactivation of human STUB1 in both tumors and immune cells could overcome ICB resistance.

Thu Aug 18 00:00:00 EDT 2022

STUB1 is an intracellular checkpoint for interferon gamma sensing.

Scientific reports

Ng S,Lim S,Sim ACN,Mangadu R,Lau A,Zhang C,Martinez SB,Chandramohan A,Lim UM,Ho SSW,Chang SC,Gopal P,Hong LZ,Schwaid A,Fernandis AZ,Loboda A,Li C,Phan U,Henry B,Partridge AW

Thu Aug 18 00:00:00 EDT 2022

Tet2 deficiency drives liver microbiome dysbiosis triggering Tc1 cell autoimmune hepatitis.

Cell host & microbe

Pandey SP,Bender MJ,McPherson AC,Phelps CM,Sanchez LM,Rana M,Hedden L,Sangani KA,Chen L,Shapira JH,Siller M,Goel C,Verdú EF,Jabri B,Chang A,Chandran UR,Mullett SJ,Wendell SG,Singhi AD,Tilstra JS,Pierre JF,Arteel GE,Hinterleitner R,Meisel M

12-1191-82 was used in Flow cytometry/Cell sorting to demonstrate that I3A is required for L. reuteri-induced Tc1 cell differentiation in vitro and AIH-like pathology in vivo, both of which are restrained by Tet2 within CD8 T cells.

Wed Jul 13 00:00:00 EDT 2022

Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.

Cancer discovery

Du W,Hua F,Li X,Zhang J,Li S,Wang W,Zhou J,Wang W,Liao P,Yan Y,Li G,Wei S,Grove S,Vatan L,Zgodziński W,Majewski M,Wallner G,Chen H,Kryczek I,Fang JY,Zou W

12-1191-82 was used in Flow Cytometry to show that optineurin is a shared node between the IFN and MHC pathways and predicts colorectal cancer patient outcome.

Thu Jul 01 00:00:00 EDT 2021

Mycolactone displays anti-inflammatory effects on the nervous system.

PLoS neglected tropical diseases

Isaac C,Mauborgne A,Grimaldi A,Ade K,Pohl M,Limatola C,Boucher Y,Demangel C,Guenin-Macé L

Published figure using CD119 (IFN gamma Receptor 1) monoclonal antibody (Product # 12-1191-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Erythropoietin treatment in murine multiple myeloma: immune gain and bone loss.

Scientific reports

Deshet-Unger N,Hiram-Bab S,Haim-Ohana Y,Mittelman M,Gabet Y,Neumann D

12-1191 was used in Flow cytometry/Cell sorting to reveal that in multiple myeloma, erythropoietin may act as a double-edged sword by stimulating the immune response whilst accelerating bone resorption.

Tue Aug 02 00:00:00 EDT 2016

iNKT Cells Are Responsible for the Apoptotic Reduction of Basophils That Mediate Th2 Immune Responses Elicited by Papain in Mice Following γPGA Stimulation.

PloS one

Park HJ,Lee SW,Park SH,Hong S

12-1191 was used in Flow cytometry/Cell sorting to demonstrate that γPGA-induced iNKT cell polarization toward the Th1 phenotype induces apoptotic basophil depletion, leading to the suppression of Th2 immune responses.

Mon Aug 22 00:00:00 EDT 2016

Chronic oral infection with major periodontal bacteria Tannerella forsythia modulates systemic atherosclerosis risk factors and inflammatory markers.

Pathogens and disease

Chukkapalli SS,Rivera-Kweh MF,Velsko IM,Chen H,Zheng D,Bhattacharyya I,Gangula PR,Lucas AR,Kesavalu L

12-1191 was used in Flow cytometry/Cell sorting to investigate the potential of a known bacterial pathogen found in atherosclerotic plaque in humans to accelerate atherosclerosis in hyperlipdemic mice.

Wed Apr 01 00:00:00 EDT 2015

CD8+ T Cells Mediate Female-Dominant IL-4 Production and Airway Inflammation in Allergic Asthma.

PloS one

Ito C,Okuyama-Dobashi K,Miyasaka T,Masuda C,Sato M,Kawano T,Ohkawara Y,Kikuchi T,Takayanagi M,Ohno I

12-1191 was used in Flow cytometry/Cell sorting to determine the mechanisms behind the sex differences in asthmatic responses after puberty.

Wed Jul 06 00:00:00 EDT 2016

Polymicrobial Oral Infection with Four Periodontal Bacteria Orchestrates a Distinct Inflammatory Response and Atherosclerosis in ApoE null Mice.

PloS one

Chukkapalli SS,Velsko IM,Rivera-Kweh MF,Zheng D,Lucas AR,Kesavalu L

12-1191 was used in Flow cytometry/Cell sorting to study the levels of inflammatory molecules and cells types involved in mounting an immune response to peridontal infection over time.

Fri Jun 17 00:00:00 EDT 2016

A role for the ITAM signaling module in specifying cytokine-receptor functions.

Nature immunology

Bezbradica JS,Rosenstein RK,DeMarco RA,Brodsky I,Medzhitov R

Published figure using CD119 (IFN gamma Receptor 1) monoclonal antibody (Product # 12-1191-82) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

A type I IFN-Flt3 ligand axis augments plasmacytoid dendritic cell development from common lymphoid progenitors.

The Journal of experimental medicine

Chen YL,Chen TT,Pai LM,Wesoly J,Bluyssen HA,Lee CK

12-1191 was used in Flow cytometry/Cell sorting to define a critical role for the FL/IFN-I/Flt3 axis in plasmacytoid dendritic cell differentiation from common lymphoid progenitors.

Mon Nov 18 00:00:00 EST 2013

Prostaglandin E₂ promotes Th1 differentiation via synergistic amplification of IL-12 signalling by cAMP and PI3-kinase.

Nature communications

Yao C,Hirata T,Soontrapa K,Ma X,Takemori H,Narumiya S

12-1191 was used in Flow cytometry/Cell sorting to investigate the role of cAMP in Th1 differentiation, showing that prostaglandin E2 promotes differentiation via synergistic amplification of IL-12 signalling by cAMP and PI3-kinase.

Tue Oct 22 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD119; IFN-gamma receptor 1; IFN-gamma-R-alpha; IFN-gamma-R1; INF-g receptor; Interferon gamma receptor 1; Interferon gamma receptor alpha-chain; interferon-gamma receptor 1

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Gene Aliases: CD119; Ifgr; IFN-gammaR; Ifngr; Ifngr1; Nktar

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UniProt ID: (Mouse) P15261

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Entrez Gene ID: (Mouse) 15979

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Function(s)

cytokine receptor activity cytokine binding transmembrane signal receptor

Process(es)

cytokine-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD119 (IFN gamma Receptor 1) Monoclonal Antibody (2E2), PE, eBioscience™

CD119 (IFN gamma Receptor 1) Antibody (12-1191-82) in Flow

Product Details

12-1191-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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