Invitrogen

CD11a Monoclonal Antibody (MEM-25)

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Antibody Testing Data (2)
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CD11a Antibody (MA1-19003) in Flow

Separation of human monocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD11a (MEM-25) purified Monoclonal antibody (Product # MA1-19003) (azide free, concentration in sample 1 µg/mL) GAM APC.

CD11a Antibody in Radioimmune assays (RIA)

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Product Details

MA1-19003

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

2 µg/mL

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

Radioimmune Assays (RIA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MEM-25

Immunogen

Leucocytes from a patient suffering from a LGL type leukaemia.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1071150

Product Specific Information

This antibody reacts with an extracellular epitope of CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.

Functional Application: Blocking.

Target Information

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Intercellular adhesion molecule 1 promotes HIV-1 attachment but not fusion to target cells.

PloS one

Kondo N,Melikyan GB

MA1-19003 was used in blocking or activating experiment to determine the role of ICAM-1/LFA-1 interactions in productive HIV-1 entry into lymphoid cells

Thu Mar 07 00:00:00 EST 2013

Gut homing receptors on CD8 T cells are retinoic acid dependent and not maintained by liver dendritic or stellate cells.

Gastroenterology

Eksteen B,Mora JR,Haughton EL,Henderson NC,Lee-Turner L,Villablanca EJ,Curbishley SM,Aspinall AI,von Andrian UH,Adams DH

MA1-19003 was used in flow cytometry to elucidate the processes that control enterohepatic T-cell migration

Wed Jul 01 00:00:00 EDT 2009

Two-dimensional kinetics regulation of alphaLbeta2-ICAM-1 interaction by conformational changes of the alphaL-inserted domain.

The Journal of biological chemistry

Zhang F,Marcus WD,Goyal NH,Selvaraj P,Springer TA,Zhu C

MA1-19003 was used in flow cytometry to study conformational changes of the leukocyte integrin alphaLbeta2

Fri Dec 23 00:00:00 EST 2005

Searching for common stem cells of the hepatic and hematopoietic systems in the human fetal liver: CD34+ cytokeratin 7/8+ cells express markers for stellate cells.

Journal of hepatology

Suskind DL,Muench MO

MA1-19003 was used in flow cytometry to test if human fetal liver cells expressing CD34 and CK7/8 represent a common stem cell for both the hematopoietic and hepatic systems

Sun Feb 01 00:00:00 EST 2004

Modulation of neutrophil function by the tripeptide feG.

BMC immunology

Mathison RD,Befus AD,Davison JS,Woodman RC

MA1-19003 was used in flow cytometry to determine the effects of feG on human and rat neutrophil function.

Tue Mar 04 00:00:00 EST 2003

Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction.

Clinical and diagnostic laboratory immunology

Hajishengallis G,Martin M,Sojar HT,Sharma A,Schifferle RE,DeNardin E,Russell MW,Genco RJ

MA1-19003 was used in flow cytometry to determine of proinflammatory cytokine responses induced by certain bacterial protein adhesins are dependent on TLRs.

Fri Mar 01 00:00:00 EST 2002

Filarial antigens impair the function of human dendritic cells during differentiation.

Infection and immunity

Semnani RT,Sabzevari H,Iyer R,Nutman TB

MA1-19003 was used in flow cytometry to assess the effect of microfilariae antigen on dendritic cells

Sat Sep 01 00:00:00 EDT 2001

Increased phagocyte Fc gammaRI expression and improved Fc gamma-receptor-mediated phagocytosis after in vivo recombinant human interferon-gamma treatment of normal human subjects.

Blood

Schiff DE,Rae J,Martin TR,Davis BH,Curnutte JT

MA1-19003 was used in flow cytometry to test if recombinant human interferon-gamma enhancing clearance of microbes in patients with chronic granulomatous disease.

Wed Oct 15 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; Integrin alpha-L; integrin alphaL; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1 alpha; LFA-1A; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide

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Gene Aliases: CD11A; ITGAL; LFA-1; LFA1A

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UniProt ID: (Human) P20701

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Entrez Gene ID: (Human) 3683

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Function(s)

protein binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

Process(es)

T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell phagocytosis movement of cell or subcellular component inflammatory response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion signal transduction integrin-mediated signaling pathway extracellular matrix organization receptor clustering regulation of immune response leukocyte migration

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CD11a Monoclonal Antibody (MEM-25)

CD11a Antibody (MA1-19003) in Flow

Product Details

MA1-19003

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Radioimmune Assays (RIA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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