Invitrogen

CD11a Monoclonal Antibody (TS1/22)

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CD11a Antibody (MA11A10) in Flow

Flow cytometry analysis of CD11 alpha in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA11A10) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight... View More

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Product Details

MA11A10

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 9 publications 9 publications

ELISA (ELISA)

View 2 publications 2 publications

Immunoprecipitation (IP)

View 4 publications 4 publications

Neutralization (Neu)

View 16 publications 16 publications

Inhibition Assays (IA)

View 2 publications 2 publications

Blocking Assay (BLOCK)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Hamster, Human, Mouse, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TS1/22

Immunogen

Human 180 kD alpha chain of the CD11alpha complex.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223513

Product Specific Information

MA11A10 targets CD11 alpha in FACS applications and shows reactivity with Human and Mouse samples.

The MA11A10 immunogen is human 180 kD alpha chain of the CD11alpha complex.

MA11A10 detects CD11 alpha which has a predicted molecular weight of approximately 126 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Increasing LFA-1 Expression Enhances Immune Synapse Architecture and T Cell Receptor Signaling in Jurkat E6.1 Cells.

Frontiers in cell and developmental biology

Cassioli C,Balint S,Compeer EB,Felce JH,Gamberucci A,Della Bella C,Felce SL,Brunetti J,Valvo S,Pende D,D'Elios MM,Moretta L,Dustin ML,Baldari CT

MA11A10 was used in Flow Cytometry to highlight a new role for LFA-1 in the core architecture of the IS that can be exploited to study the spatiotemporal redistribution of surface receptors on SLBs, thereby extending the potential of E6.1 cells and their derivatives for fine-scale imaging studies.

Mon Sep 05 00:00:00 EDT 2022

The kinetics of E-selectin- and P-selectin-induced intermediate activation of integrin αLβ2 on neutrophils.

Journal of cell science

Zhou F,Zhang F,Zarnitsyna VI,Doudy L,Yuan Z,Li K,McEver RP,Lu H,Zhu C

MA11A10 was used in Inhibition Assays to define the precise timing and kinetics of intermediate activation of αLβ2 by E- and P-selectins.

Wed Sep 15 00:00:00 EDT 2021

Anti-αLβ2 antibodies reveal novel endocytotic cross-modulatory functionality.

British journal of pharmacology

Mancuso RV,Casper J,Schmidt AG,Krähenbühl S,Weitz-Schmidt G

MA11A10 was used in Flow Cytometry to demonstrate that anti-αLβ2 mAbs directly induce the internalization of α4 integrins.

Mon Jun 01 00:00:00 EDT 2020

VLA-4 phosphorylation during tumor and immune cell migration relies on its coupling to VEGFR2 and CXCR4 by syndecan-1.

Journal of cell science

Jung O,Beauvais DM,Adams KM,Rapraeger AC

MA11A10 was used in Western Blotting to demonstrate that VEGFR2 acts as a scaffold for the entire machinery necessary for phosphorylation of VLA-4 on S988.

Mon Oct 28 00:00:00 EDT 2019

PRDM4 mediates YAP-induced cell invasion by activating leukocyte-specific integrin β2 expression.

EMBO reports

Liu H,Dai X,Cao X,Yan H,Ji X,Zhang H,Shen S,Si Y,Zhang H,Chen J,Li L,Zhao JC,Yu J,Feng XH,Zhao B

Published figure using CD11a monoclonal antibody (Product # MA11A10) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

LFA-1 and Mac-1 integrins bind to the serine/threonine-rich domain of thrombomodulin.

Biochemical and biophysical research communications

Kawamoto E,Okamoto T,Takagi Y,Honda G,Suzuki K,Imai H,Shimaoka M

MA11A10 was used in blocking or activating experiment to report that LFA-1 and Mac-1 on leukocytes bind to thrombomodulin

Fri May 13 00:00:00 EDT 2016

Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell-mediated cytotoxicity.

Molecular biology of the cell

Tuli A,Thiery J,James AM,Michelet X,Sharma M,Garg S,Sanborn KB,Orange JS,Lieberman J,Brenner MB

MA11A10 was used in flow cytometry to identify ADP-ribosylation factor-like 8b as a factor required for natural killer cell-mediated cytotoxicity

Sun Dec 01 00:00:00 EST 2013

Dynamic alterations in chemokine gradients induce transendothelial shuttling of human T cells under physiologic shear conditions.

Journal of leukocyte biology

Lee JY,Buzney CD,Poznansky MC,Sackstein R

MA11A10 was used in Inhibition Assays to characterise the molecular effectors of the active movement of cells from subendothelial compartments into the bloodstream.

Tue Dec 01 00:00:00 EST 2009

Inflamed lymphatic endothelium suppresses dendritic cell maturation and function via Mac-1/ICAM-1-dependent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Podgrabinska S,Kamalu O,Mayer L,Shimaoka M,Snoeck H,Randolph GJ,Skobe M

MA11A10 was used in blocking or activating experiment to investigate the suppresive effect of inflamed lymphatic endothelium on dendritic cell maturation and its mechanism

Sat Aug 01 00:00:00 EDT 2009

Dual mode of HMG-CoA reductase inhibition on dendritic cell invasion.

Atherosclerosis

Kofler S,Schlichting C,Jankl S,Nickel T,Weis M

MA11A10 was used in blocking/activating experiment to study the mechanism for the effect inhibitor HMG-CoA reductase on dendritic cell invasion

Sat Mar 01 00:00:00 EST 2008

Adhesion of Epstein-Barr virus-positive natural killer cell lines to cultured endothelial cells stimulated with inflammatory cytokines.

Clinical and experimental immunology

Kanno H,Watabe D,Shimizu N,Sawai T

MA11A10 was used in flow cytometry to study the effect of inflammatory cytokines on the adhesion of NK cells to endothelial cells

Sat Mar 01 00:00:00 EST 2008

High-mobility group box 1 activates integrin-dependent homing of endothelial progenitor cells.

Circulation research

Chavakis E,Hain A,Vinci M,Carmona G,Bianchi ME,Vajkoczy P,Zeiher AM,Chavakis T,Dimmeler S

MA11A10 was used in Neutralization to establish a link between HMGB1 and integrin functions of EPCs and demonstrate that HMGB1 stimulates EPC homing to ischemic tissues.

Fri Feb 02 00:00:00 EST 2007

Adhesion of peripheral blood mononuclear cells and CD4+ T cells from patients with psoriasis to cultured endothelial cells via the interaction between lymphocyte function-associated antigen type 1 and intercellular adhesion molecule 1.

The British journal of dermatology

Watabe D,Kanno H,Yoshida A,Kurose A,Akasaka T,Sawai T

MA11A10 was used in blocking/activating experiment to study the adhesion of PBMC and CD4+ T cells from psoriasis patients to cultured endothelial cells

Wed Aug 01 00:00:00 EDT 2007

Recruitment of dynein to the Jurkat immunological synapse.

Proceedings of the National Academy of Sciences of the United States of America

Combs J,Kim SJ,Tan S,Ligon LA,Holzbaur EL,Kuhn J,Poenie M

MA11A10 was used in ELISA to investigate the formation of the Jurkat immunological synapse

Tue Oct 03 00:00:00 EDT 2006

Talin1 regulates TCR-mediated LFA-1 function.

Journal of immunology (Baltimore, Md. : 1950)

Simonson WT,Franco SJ,Huttenlocher A

MA11A10 was used in blocking/activating experiment to investigate the role of Talin 1 in the regulation of LFA-1 by TCR

Fri Dec 01 00:00:00 EST 2006

Human macrophages rescue myoblasts and myotubes from apoptosis through a set of adhesion molecular systems.

Journal of cell science

Sonnet C,Lafuste P,Arnold L,Brigitte M,Poron F,Authier FJ,Chrétien F,Gherardi RK,Chazaud B

MA11A10 was used in blocking or activating experiment to study the mechanisms by which human macrophages rescue myoblasts and myotubes from apoptosis

Thu Jun 15 00:00:00 EDT 2006

A novel flow-cytometry-based assay for quantification of affinity and avidity changes of integrins.

Journal of immunological methods

Konstandin MH,Sester U,Klemke M,Weschenfelder T,Wabnitz GH,Samstag Y

MA11A10 was used in blocking/activating experiment to investigate the reliability of a novel flow-cytometry-based assay for quantification of affinity and avidity changes of integrins

Mon Mar 20 00:00:00 EST 2006

Human CD1-restricted T cell recognition of lipids from pollens.

The Journal of experimental medicine

Agea E,Russano A,Bistoni O,Mannucci R,Nicoletti I,Corazzi L,Postle AD,De Libero G,Porcelli SA,Spinozzi F

MA11A10 was used in flow cytometry to study the immune response of pollen lipids

Mon Jul 18 00:00:00 EDT 2005

Monocyte adhesion to xenogeneic endothelium during laminar flow is dependent on alpha-Gal-mediated monocyte activation.

Journal of immunology (Baltimore, Md. : 1950)

Peterson MD,Jin R,Hyduk S,Duchesneau P,Cybulsky MI,Waddell TK

MA11A10 was used in flow cytometry to investigate the mechanism of the monocyte interaction with xenogeneic endothelium

Wed Jun 15 00:00:00 EDT 2005

Dendritic cell adhesion is enhanced on endothelial cells preexposed to calcineurin inhibitors.

Journal of cardiovascular pharmacology

Schlichting CL,Schareck WD,Weis M

MA11A10 was used in blocking/activating experiment to investigate the influence of calcineurin inhibitors on dendritic cell adhesion and transmigration to allogeneic endothelial cells

Thu Sep 01 00:00:00 EDT 2005

Transendothelial migration of human basophils.

Journal of immunology (Baltimore, Md. : 1950)

Iikura M,Ebisawa M,Yamaguchi M,Tachimoto H,Ohta K,Yamamoto K,Hirai K

MA11A10 was used in blocking/activating experiment to investigate the basophil transendothelial migration

Fri Oct 15 00:00:00 EDT 2004

Phenyl methimazole inhibits TNF-alpha-induced VCAM-1 expression in an IFN regulatory factor-1-dependent manner and reduces monocytic cell adhesion to endothelial cells.

Journal of immunology (Baltimore, Md. : 1950)

Dagia NM,Harii N,Meli AE,Sun X,Lewis CJ,Kohn LD,Goetz DJ

MA11A10 was used in ELISA to study the effect of phenyl methimazole on tumor necrosis factor-alpha-induced VCAM-1 expression and its mechanism

Sun Aug 01 00:00:00 EDT 2004

Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils.

The Journal of biological chemistry

Zen K,Utech M,Liu Y,Soto I,Nusrat A,Parkos CA

MA11A10 was used in immunoprecipitation to investigate the interaction between BAP31 and CD11b/CD18

Fri Oct 22 00:00:00 EDT 2004

Vav1 phosphorylation is induced by beta2 integrin engagement on natural killer cells upstream of actin cytoskeleton and lipid raft reorganization.

The Journal of experimental medicine

Riteau B,Barber DF,Long EO

MA11A10 was used in blocking/activating experiment to investigate the role of LFA-1 for the regulation of Vav1 in NK cells

Mon Aug 04 00:00:00 EDT 2003

Human natural killer cell activity against porcine targets: modulation by control of the oxidation-reduction environment and role of adhesion molecule interactions.

Cellular immunology

Horvath-Arcidiacono JA,Tsuyuki S,Mostowski H,Bloom ET

MA11A10 was used in blocking/activating experiment to investigate the effect of redox in the human organ transplantation from pigs

Sat Mar 01 00:00:00 EST 2003

CD2 molecules redistribute to the uropod during T cell scanning: implications for cellular activation and immune surveillance.

Proceedings of the National Academy of Sciences of the United States of America

Tibaldi EV,Salgia R,Reinherz EL

MA11A10 was used in blocking/activating experiment to study the redistribution of CD2 to the uropod in T cell scanning

Tue May 28 00:00:00 EDT 2002

Endothelial determinants of dendritic cell adhesion and migration: new implications for vascular diseases.

Arteriosclerosis, thrombosis, and vascular biology

Weis M,Schlichting CL,Engleman EG,Cooke JP

MA11A10 was used in blocking/activating experiment to investigate the mechanism for dendritic cell adhesion and migration

Fri Nov 01 00:00:00 EST 2002

CD11b/CD18-coated microspheres attach to E-selectin under flow.

Journal of leukocyte biology

Crutchfield KL,Shinde Patil VR,Campbell CJ,Parkos CA,Allport JR,Goetz DJ

MA11A10 was used in flow cytometry to study the interaction between E-selectin and CD11b/CD18

Tue Feb 01 00:00:00 EST 2000

ICAM-3 (CD50) is expressed by human mast cells: induction of homotypic mast cell aggregation via ICAM-3.

Cell adhesion and communication

Babina M,Mammeri K,Henz BM

MA11A10 was used in blocking/activating experiment to investigate the characteristics of ICAM3 in human mast cells

Tue Feb 01 00:00:00 EST 2000

Fever-range hyperthermia enhances L-selectin-dependent adhesion of lymphocytes to vascular endothelium.

Journal of immunology (Baltimore, Md. : 1950)

Wang WC,Goldman LM,Schleider DM,Appenheimer MM,Subjeck JR,Repasky EA,Evans SS

MA11A10 was used in flow cytometry to investigate the role of L-selectin-dependent adhesion

Thu Jan 15 00:00:00 EST 1998

Mapping the intercellular adhesion molecule-1 and -2 binding site on the inserted domain of leukocyte function-associated antigen-1.

The Journal of biological chemistry

Edwards CP,Fisher KL,Presta LG,Bodary SC

MA11A10 was used in immunocytochemistry to mapp the binding site for ICAM-1 and -2 on LFA-1 inserted domain

Fri Oct 30 00:00:00 EST 1998

Isolation and characterization of cell lines with genetically distinct mutations downstream of protein kinase C that result in defective activation-dependent regulation of T cell integrin function.

Journal of immunology (Baltimore, Md. : 1950)

Mobley JL,Ennis E,Shimizu Y

MA11A10 was used in blocking/activating experiment to characterize two cells with defective T cell integrin function

Thu Feb 01 00:00:00 EST 1996

Respiratory syncytial virus infection enhances neutrophil and eosinophil adhesion to cultured respiratory epithelial cells. Roles of CD18 and intercellular adhesion molecule-1.

Journal of immunology (Baltimore, Md. : 1950)

Stark JM,Godding V,Sedgwick JB,Busse WW

MA11A10 was used in blocking/activating experiment to investigate the effect of ICAM 1 and CD18 on the aggregation of white cells with RSV infection

Sat Jun 15 00:00:00 EDT 1996

Cloning of the beta subunit of the leukocyte adhesion proteins: homology to an extracellular matrix receptor defines a novel supergene family.

Cell

Kishimoto TK,O'Connor K,Lee A,Roberts TM,Springer TA

MA11A10 was used in immunoprecipitation to purify and clone the beta subunit of LFA-1, Mac-1 and other leukocyte adhesion proteins

Fri Feb 27 00:00:00 EST 1987

Heterogeneous mutations in the beta subunit common to the LFA-1, Mac-1, and p150,95 glycoproteins cause leukocyte adhesion deficiency.

Cell

Kishimoto TK,Hollander N,Roberts TM,Anderson DC,Springer TA

MA11A10 was used in immunoprecipitation to study the effect of mutations in the LFA-1, Mac-1 and p159 common beta subunit on leukocyte adhesion

Fri Jul 17 00:00:00 EDT 1987

A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.

The Journal of experimental medicine

Sanchez-Madrid F,Nagy JA,Robbins E,Simon P,Springer TA

MA11A10 was used in immunoprecipitation to investigate the relationship among the subunits of leukocyte cell surface antigens

Thu Dec 01 00:00:00 EST 1983

Bioinformatics

Protein Aliases: alpha L integrin; antigen CD11A (p180); antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; antigen CD11A p180; CD11 antigen-like family member A; CD11a; integrin alpha L; Integrin alpha-L; integrin alphaL; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1 alpha; LFA-1A; Ly-15; Lymphocyte antigen 15; lymphocyte function associated antigen 1, alpha polypeptide; lymphocyte function-associated antigen 1; lymphocyte function-associated antigen 1, alpha polypeptide

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Gene Aliases: (p180); CD11A; ITGAL; LFA-1; LFA-1A; LFA1A; Ly-15; Ly-21

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UniProt ID: (Human) P20701, (Mouse) P24063

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Entrez Gene ID: (Human) 3683, (Mouse) 16408

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Function(s)

protein binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

Process(es)

T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell phagocytosis movement of cell or subcellular component inflammatory response cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion signal transduction integrin-mediated signaling pathway extracellular matrix organization receptor clustering regulation of immune response leukocyte migration cell surface receptor signaling pathway single organismal cell-cell adhesion regulation of cell-cell adhesion positive regulation of cell-cell adhesion positive regulation of T cell proliferation activated T cell proliferation positive regulation of calcium-mediated signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD11a Monoclonal Antibody (TS1/22)

CD11a Antibody (MA11A10) in Flow

Product Details

MA11A10

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Inhibition Assays (IA)

Blocking Assay (BLOCK)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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