Invitrogen

CD11b Monoclonal Antibody (M1/70.15), FITC

1 Published Figure

23 References

View all (223) CD11b antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

FIGURE: 1 / 2

CD11b Antibody (MA5-17859) in Flow

Flow cytometric analysis of C3H/HE mouse peritoneal macrophages (left) and bone marrow (right) were stained using a CD11b/ITGAM/Integrin alpha M monoclonal antibody (Product # MA5-17859) (filled histogram) or rat IgG2b isotype control (open histogram).

Product Image Gallery

Product Details

MA5-17859

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 7 publications 7 publications

Miscellaneous PubMed (Misc)

View 15 publications 15 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

M1/70.15

Immunogen

C57BL/6 spleen cell enriched for T lymphocytes

View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4-5mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539243

Product Specific Information

MA5-17859 targets CD11b/ITGAM/Integrin alpha M in FACS applications and shows reactivity with Mouse samples.

The MA5-17859 immunogen is c57BL/6 spleen cell enriched for T lymphocytes.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Traumatic brain injury stimulates sympathetic tone-mediated bone marrow myelopoiesis to favor fracture healing.

Signal transduction and targeted therapy

Liu W,Chen W,Xie M,Chen C,Shao Z,Zhang Y,Zhao H,Song Q,Hu H,Xing X,Cai X,Deng X,Li X,Wang P,Liu G,Xiong L,Lv X,Zhang Y

Published figure using CD11b monoclonal antibody (Product # MA5-17859) in Flow Cytometry

Wed Jul 05 00:00:00 EDT 2023

Traumatic brain injury stimulates sympathetic tone-mediated bone marrow myelopoiesis to favor fracture healing.

Signal transduction and targeted therapy

Liu W,Chen W,Xie M,Chen C,Shao Z,Zhang Y,Zhao H,Song Q,Hu H,Xing X,Cai X,Deng X,Li X,Wang P,Liu G,Xiong L,Lv X,Zhang Y

Published figure using CD11b monoclonal antibody (Product # MA5-17859) in Flow Cytometry

Wed Jul 05 00:00:00 EDT 2023

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

MA5-17859 was used in Immunohistochemistry to describe a protocol for generating functional T cells from Hoxb5-expressing B cells in vivo.

Sat Sep 01 00:00:00 EDT 2018

Conventional bone marrow-derived dendritic cells contribute to toll-like receptor-independent production of alpha/beta interferon in response to inactivated parapoxvirus ovis.

Journal of virology

Siegemund S,Hartl A,von Buttlar H,Dautel F,Raue R,Freudenberg MA,Fejer G,Büttner M,Köhler G,Kirschning CJ,Sparwasser T,Alber G

MA5-17859 was used in flow cytometry to study the effect of inactivated Parapoxvirus ovis on dendritic cells

Tue Sep 01 00:00:00 EDT 2009

Role of T cells in innate and adaptive immunity against murine Burkholderia pseudomallei infection.

The Journal of infectious diseases

Haque A,Easton A,Smith D,O'Garra A,Van Rooijen N,Lertmemongkolchai G,Titball RW,Bancroft GJ

MA5-17859 was used in flow cytometry to study the in vivo role of cytokine- and antigen-mediated T cell activation in the resistance to Burkholderia pseudomallei

Wed Feb 01 00:00:00 EST 2006

TLR4, TLR9 and MyD88 are not required for the positive selection of autoreactive B cells into the primary repertoire.

European journal of immunology

Silver K,Ferry H,Crockford T,Cornall RJ

MA5-17859 was used in flow cytometry to demonstrate that the effects of Toll-like receptors on autoimmunity occur in the established repertoire and not during B cell development

Thu Jun 01 00:00:00 EDT 2006

Tick saliva inhibits differentiation, maturation and function of murine bone-marrow-derived dendritic cells.

Immunology

Cavassani KA,Aliberti JC,Dias AR,Silva JS,Ferreira BR

MA5-17859 was used in flow cytometry to assess the effects of Rhipicephalus sanguineus tick saliva on dendritic cell differentiation and maturation

Tue Feb 01 00:00:00 EST 2005

Time-course expression of Toll-like receptors 2 and 4 in inflammatory bowel disease and homeostatic effect of VIP.

Journal of leukocyte biology

Gomariz RP,Arranz A,Abad C,Torroba M,Martinez C,Rosignoli F,Garcia-Gómez M,Leceta J,Juarranz Y

MA5-17859 was used in flow cytometry to study TLR2 and 4 expression in a trinitrobenzene sulfonic acid-induced colitis model of Crohn's disease

Mon Aug 01 00:00:00 EDT 2005

Modulation of interferon-induced genes by lipoxin analogue in anti-glomerular basement membrane nephritis.

Journal of the American Society of Nephrology : JASN

Ohse T,Ota T,Kieran N,Godson C,Yamada K,Tanaka T,Fujita T,Nangaku M

MA5-17859 was used in flow cytometry to assess the effect of aspirin-triggered 15-epi-lipoxin A4 in experimental anti-glomerular basement membrane antibody nephritis

Thu Apr 01 00:00:00 EST 2004

Characterization of the mouse analogues of CD59 using novel monoclonal antibodies: tissue distribution and functional comparison.

Immunology

Harris CL,Hanna SM,Mizuno M,Holt DS,Marchbank KJ,Morgan BP

MA5-17859 was used in flow cytometry to determine the expression of CD59a and CD59b.

Thu May 01 00:00:00 EDT 2003

Macrophage C-type lectin on bone marrow-derived immature dendritic cells is involved in the internalization of glycosylated antigens.

Glycobiology

Denda-Nagai K,Kubota N,Tsuiji M,Kamata M,Irimura T

MA5-17859 was used in flow cytometry to study expression of a C-type lectin specific for galactose and N-acetylgalactosamine on murine dendritic cells.

Mon Jul 01 00:00:00 EDT 2002

Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G (Gr1(+))CD11b(+ )immature myeloid suppressor cells.

International immunology

Goñi O,Alcaide P,Fresno M

MA5-17859 was used in flow cytometry to elucidate the immunosuppressive mechanism that occurs during T. cruzi infection.

Tue Oct 01 00:00:00 EDT 2002

Candida albicans infection enhances immunosuppression induced by cyclophosphamide by selective priming of suppressive myeloid progenitors for NO production.

Cellular immunology

Angulo I,Jiménez-Díaz MB,García-Bustos JF,Gargallo D,de las Heras FG,Muñoz-Fernández MA,Fresno M

MA5-17859 was used in flow cytometry to examine the T cell responses in cyclophosphamide-treated mice after systemic infection with Candida albicans.

Wed Feb 05 00:00:00 EST 2003

Nitric oxide-producing CD11b(+)Ly-6G(Gr-1)(+)CD31(ER-MP12)(+) cells in the spleen of cyclophosphamide-treated mice: implications for T-cell responses in immunosuppressed mice.

Blood

Angulo I,de las Heras FG,García-Bustos JF,Gargallo D,Muñoz-Fernández MA,Fresno M

MA5-17859 was used in flow cytometry to study T-cell unresponsiveness in cyclophosphamide treated mice.

Sat Jan 01 00:00:00 EST 2000

Identification of an early T cell progenitor for a pathway of T cell maturation in the bone marrow.

Proceedings of the National Academy of Sciences of the United States of America

Dejbakhsh-Jones S,Strober S

MA5-17859 was used in flow cytometry to identify a rare population of cells in the adult mouse bone marrow that generates CD4(+) and CD8(+) TCRalphabeta(+) T cells.

Tue Dec 07 00:00:00 EST 1999

Immunophenotypical and functional heterogeneity of dendritic cells generated from murine bone marrow cultured with different cytokine combinations: implications for anti-tumoral cell therapy.

Immunology

Masurier C,Pioche-Durieu C,Colombo BM,Lacave R,Lemoine FM,Klatzmann D,Guigon M

MA5-17859 was used in flow cytometry to characterize in vitro-generated dendritic cells.

Thu Apr 01 00:00:00 EST 1999

Cellular and molecular characterization of the scurfy mouse mutant.

Journal of immunology (Baltimore, Md. : 1950)

Clark LB,Appleby MW,Brunkow ME,Wilkinson JE,Ziegler SF,Ramsdell F

MA5-17859 was used in flow cytometry to further characterize mice hemizygous for scurfy.

Mon Mar 01 00:00:00 EST 1999

Three populations of mouse lymph node dendritic cells with different origins and dynamics.

Journal of immunology (Baltimore, Md. : 1950)

Salomon B,Cohen JL,Masurier C,Klatzmann D

MA5-17859 was used in flow cytometry to characterize different populations of mouse lymph node dendritic cells.

Thu Jan 15 00:00:00 EST 1998

The preclinical pharmacological profile of the potent and selective leukotriene B4 antagonist CP-195543.

The Journal of pharmacology and experimental therapeutics

Showell HJ,Conklyn MJ,Alpert R,Hingorani GP,Wright KF,Smith MA,Stam E,Salter ED,Scampoli DN,Meltzer S,Reiter LA,Koch K,Piscopio AD,Cortina SR,Lopez-Anaya A,Pettipher ER,Milici AJ,Griffiths RJ

MA5-17859 was used in flow cytometry to characterize a selective and potent leukotriene B4 receptor antagonist.

Mon Jun 01 00:00:00 EDT 1998

Immunologic memory induced by a glycoconjugate vaccine in a murine adoptive lymphocyte transfer model.

Infection and immunity

Guttormsen HK,Wetzler LM,Finberg RW,Kasper DL

MA5-17859 was used in flow cytometry to develop a mouse model to study the ability of a glycoprotein conjugate vaccine to induce immunologic memory for the polysaccharide moiety.

Fri May 01 00:00:00 EDT 1998

Mechanisms of allografted tumor rejection: the roles of T cells in allograft rejection mediated by a type of bone marrow-derived macrophage.

Microbiology and immunology

Ushio-Umeda Y,Yoshida R

MA5-17859 was used in flow cytometry to elucidate the roles of T cells in the AIM-mediated rejection of tumor cells.

Thu Apr 02 00:00:00 EST 1998

Thy.1lowCD3- cells sorted from nylon wool-passed bone marrow cells can augment the H-2 identical but not non-identical cytotoxic T lymphocyte precursors in mixed lymphocyte cultures.

Microbiology and immunology

Mukouyama H,Tanabe MJ

MA5-17859 was used in flow cytometry to characterize T cells obtained from nylon wool-passed murine bone marrow.

Tue May 23 00:00:00 EDT 1995

CD3+ and CD4+ cells adoptively transfer experimental hypersensitivity pneumonitis.

The American review of respiratory disease

Schuyler M,Gott K,Shopp G,Crooks L

MA5-17859 was used in flow cytometry to describe the cells that transfer adoptive murine experimental hypersensitivity pneumonitis.

Tue Dec 01 00:00:00 EST 1992

Mononuclear phagocytes: a major population of effector cells responsible for rejection of allografted tumor cells in mice.

Proceedings of the National Academy of Sciences of the United States of America

Yoshida R,Takikawa O,Oku T,Habara-Ohkubo A

MA5-17859 was used in flow cytometry to examine the cells that infiltrated into the rejection site of allografted tumors.

Fri Feb 15 00:00:00 EST 1991

Bioinformatics

Protein Aliases: CD11 antigen-like family member B; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; integrin alpha-M; leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; MGC117044

View more View less

Gene Aliases: Cd11b; CD11b/CD18; CR3; CR3A; F730045J24Rik; Ly-40; Mac-1; Mac-1a; MAC1

View more View less

Entrez Gene ID: (Mouse) 16409

View more View less

Function(s)

opsonin binding glycoprotein binding heparin binding heparan sulfate proteoglycan binding metal ion binding protein heterodimerization activity integrin

Process(es)

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion integrin-mediated signaling pathway microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD11b Monoclonal Antibody (M1/70.15), FITC

CD11b Antibody (MA5-17859) in Flow

Product Details

MA5-17859

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!