Invitrogen

CD11b Monoclonal Antibody (VIM12), FITC

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

5 References

View all (219) CD11b antibodies

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Datasheet

Protocols

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FIGURE: 1 / 4

CD11b Antibody (CD11B01) in Flow

Antigen Specificity: According to the literature this antibody recognizes the CD11b antigen. CD11b associates with CD18 to form the heterodimeric complex known as Mac-1. This complex serves as a receptor for the iC3b component. Mac-1 also serves as an adhesion molecule for intracellular adhesion molecule-1, also known as CD54. Mac-1 is expressed on cells of the myeloid lineage as well as natural killer cells.

Product Image Gallery

CD11b Antibody — Figure 3. Effect of PEITC on MDSCs. Peripheral blood mononuclear cells (PBMCs) were collected from the blood obtained from control and PEITC treated MDA-MB-231 tumor-bearing SCID/NSG mice (n = 3). Modulation of CD33, CD34 and Cd11b was analyzed by immunostaining and fluorescence cytometry to determine the effects of PEITC on myeloid derived suppressor cells (MDSCs); statistical analysis was performed by Student's t test. ( A ) Bar charts showing mean channel intensities for CD33, CD34 or CD11b staining on monocytes. ( B ) Histograms for monocytes positive for CD33, CD34 or CD11b. ( C - E ) Flow cytometry showing monocytes positive for CD34 ( C ), CD33 ( D ) or CD11b ( E ) respectively in control and PEITC treated samples. {TM}

Product Details

CD11B01

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

VIM12

Immunogen

Human CD11b (Mac-1)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536479

Product Specific Information

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

Analyte Specific Reagent

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Product References

SUMOylation disassembles the tetrameric pyruvate kinase M2 to block myeloid differentiation of leukemia cells.

Cell death & disease

Xia L,Jiang Y,Zhang XH,Wang XR,Wei R,Qin K,Lu Y

CD11b01 was used in Flow cytometry/Cell sorting to study whether PKM2 is an essential modulator of leukemia cell differentiation and a potential therapeutic target, which may offer synergistic effect with differentiation therapy in the treatment of leukemia.

Wed Jan 20 00:00:00 EST 2021

SUMOylation disassembles the tetrameric pyruvate kinase M2 to block myeloid differentiation of leukemia cells.

Cell death & disease

Xia L,Jiang Y,Zhang XH,Wang XR,Wei R,Qin K,Lu Y

Wed Jan 20 00:00:00 EST 2021

Biochemical and pharmacological characterization of AZD1981, an orally available selective DP2 antagonist in clinical development for asthma.

British journal of pharmacology

Schmidt JA,Bell FM,Akam E,Marshall C,Dainty IA,Heinemann A,Dougall IG,Bonnert RV,Sargent CA

CD11B01 was used in flow cytometry to identify and characterize a novel DP2 receptor antagonist.

Mon Apr 01 00:00:00 EDT 2013

C1q Binding to and Uptake of Apoptotic Lymphocytes by Human Monocyte-derived Macrophages.

Bio-protocol

Benoit ME,Clarke EV,Tenner AJ

CD11b01 was used in Flow cytometry/Cell sorting to characterise macrophage gene expression profiles during the uptake of autologous apoptotic cells.

Thu Sep 05 00:00:00 EDT 2013

Leptin enhances the recruitment of endothelial progenitor cells into neointimal lesions after vascular injury by promoting integrin-mediated adhesion.

Circulation research

Schroeter MR,Leifheit M,Sudholt P,Heida NM,Dellas C,Rohm I,Alves F,Zientkowska M,Rafail S,Puls M,Hasenfuss G,Konstantinides S,Schäfer K

CD11b01 was used in Flow cytometry/Cell sorting to analyse the effects of leptin on human endothelial progenitor function in vitro and in vivo.

Fri Aug 29 00:00:00 EDT 2008

Directed differentiation of human embryonic stem cells to dendritic cells.

Methods in molecular biology (Clifton, N.J.)

Vodyanik MA,Slukvin II

CD11b01 was used in Flow cytometry/Cell sorting to describe a three-step cell culture method for directed differentiation of human embryonic stem cells (hESCs) to dendritic cells (DCs), useful for studying cellular and molecular mechanisms of DC development and potentially for the generation of antigen-presenting cells for cellular immunotherapy.

Thu Jun 05 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); Leukocyte adhesion receptor MO1; Mac-1 alpha (Mac1A); macrophage antigen alpha polypeptide; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit

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Gene Aliases: CD11B; CR3A; ITGAM; MAC-1; MAC1A; MO1A; SLEB6

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UniProt ID: (Human) P11215

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Entrez Gene ID: (Human) 3684

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Function(s)

glycoprotein binding protein binding metal ion binding integrin

Process(es)

cell adhesion integrin-mediated signaling pathway ectodermal cell differentiation extracellular matrix organization toll-like receptor 4 signaling pathway leukocyte migration

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CD11b Monoclonal Antibody (VIM12), FITC

CD11b Antibody (CD11B01) in Flow

Product Details

CD11B01

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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