Invitrogen

CD11b/c Monoclonal Antibody (OX-42)

1 Published Figure

24 References

View all (16) CD11b/c antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (1)

CD11b/c Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 1

CD11b/c Antibody (MA1-81606) in ICC/IF

PloS one 2016 - Published figure using CD11b/c monoclonal antibody (Product # MA1-81606) in Immunocytochemistry

Product Image Gallery

Product Details

MA1-81606

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

1:50-1:100

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

1:50-1:100

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 12 publications 12 publications

Product Specifications

Species Reactivity

Rat

Published species

Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OX-42

Immunogen

Rat peritoneal macrophages.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_927470

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Rat CD11b, clone OX-42, recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats.

Mouse anti Rat CD11b, clone OX-42, recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats.

Target Information

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. CD11b is also a receptor for fibrogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% thymocytes and is commonly used as a microgial marker in nervous tissue. CD11c encodes the integrin alpha X chain protein. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Mutations affecting the CD11b/c gene lead to systemic lupus erythematosus 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Sustained peripheral immune hyper-reactivity (SPIHR): an enduring biomarker of altered inflammatory responses in adult rats after perinatal brain injury.

Journal of neuroinflammation

Kitase Y,Chin EM,Ramachandra S,Burkhardt C,Madurai NK,Lenz C,Hoon AH,Robinson S,Jantzie LL

MA1-81606 was used in Flow Cytometry to study the persistence and time course of peripheral immune hyper-reactivity in an established and translational model of perinatal brain injury secondary to chorioamnionitis.

Tue Oct 19 00:00:00 EDT 2021

In vitro enhancement and functional characterization of neurite outgrowth by undifferentiated adipose-derived stem cells.

International journal of molecular medicine

Bucan V,Fliess M,Schnabel R,Peck CT,Vaslaitis D,Fülbier A,Reimers K,Strauss S,Vogt PM,Radtke C

MA1-81606 was used in Flow cytometry/Cell sorting to show neurite outgrowth of dorsal root ganglion neurons is directed towards undifferentiated adipose-derived stem cells.

Tue Jan 01 00:00:00 EST 2019

Differential Expression of Neuroinflammatory mRNAs in the Rat Sciatic Nerve Following Chronic Constriction Injury and Pain-Relieving Nanoemulsion NSAID Delivery to Infiltrating Macrophages.

International journal of molecular sciences

Stevens AM,Liu L,Bertovich D,Janjic JM,Pollock JA

MA1-81606 was used in Immunohistochemistry to the prognostic significance of the LMR associated with RT in patients with WR-B-NHL and acknowledged the potential use of PD-1 antibody in RT-treated lymphomas.

Thu Oct 24 00:00:00 EDT 2019

Increased ceruloplasmin expression caused by infiltrated leukocytes, activated microglia, and astrocytes in injured female rat spinal cords.

Journal of neuroscience research

Wu Y,Shen L,Wang R,Tang J,Ding SQ,Wang SN,Guo XY,Hu JG,Lü HZ

MA1-81606 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunoprecipitation to show how quantitative analysis proved that infiltrated leukocytes, activated microglia/macrophages, and astrocytes should be the major sources of increased Cp.

Sun Jul 01 00:00:00 EDT 2018

Fluoxetine prevents oligodendrocyte cell death by inhibiting microglia activation after spinal cord injury.

Journal of neurotrauma

Lee JY,Kang SR,Yune TY

MA1-81606 was used in immunohistochemistry - frozen section to demonstrate that fluoxetine alleviates oligodendrocyte cell death after spinal cord injury

Fri May 01 00:00:00 EDT 2015

A Novel Dynamic Neonatal Blood-Brain Barrier on a Chip.

PloS one

Deosarkar SP,Prabhakarpandian B,Wang B,Sheffield JB,Krynska B,Kiani MF

Published figure using CD11b/c monoclonal antibody (Product # MA1-81606) in Immunofluorescence

Mon Jun 13 00:00:00 EDT 2016

Intraperitoneal but not intravenous cryopreserved mesenchymal stromal cells home to the inflamed colon and ameliorate experimental colitis.

PloS one

Castelo-Branco MT,Soares ID,Lopes DV,Buongusto F,Martinusso CA,do Rosario A,Souza SA,Gutfilen B,Fonseca LM,Elia C,Madi K,Schanaider A,Rossi MI,Souza HS

MA1-81606 was used in flow cytometry to compare the homing and therapeutic action of cryopreserved subcutaneous adipose tissue and bone marrow-derived mesenchymal stromal cells in a rat model of colitis

Fri Jul 13 00:00:00 EDT 2012

A rat model of mild cognitive impairment associated with vascular factor.

Neuropathology : official journal of the Japanese Society of Neuropathology

Zhang L,Wei WS,Li YJ,Wang Y

MA1-81606 was used in immunohistochemistry - free floating to develop and characterize a rat model of vascular mild cognitive impairment

Fri Apr 01 00:00:00 EDT 2011

The optimal concentration of siRNA for gene silencing in primary cultured astrocytes and microglial cells of rats.

Korean journal of anesthesiology

Ki KH,Park DY,Lee SH,Kim NY,Choi BM,Noh GJ

MA1-81606 was used in immunocytochemistry to study the optimal siRNA concentration for gene silencing in both astrocytes and microglial cells.

Wed Dec 01 00:00:00 EST 2010

The parkinsonian neurotoxin rotenone activates calpain and caspase-3 leading to motoneuron degeneration in spinal cord of Lewis rats.

Neuroscience

Samantaray S,Knaryan VH,Guyton MK,Matzelle DD,Ray SK,Banik NL

MA1-81606 was used in immunohistochemistry to use the rotenone-induced model of Parkinsonism to explore spinal cord degeneration

Fri May 11 00:00:00 EDT 2007

Spinal p38beta isoform mediates tissue injury-induced hyperalgesia and spinal sensitization.

Journal of neurochemistry

Svensson CI,Fitzsimmons B,Azizi S,Powell HC,Hua XY,Yaksh TL

MA1-81606 was used in immunohistochemistry (frozen) to characterize two p38 isoforms found in the spinal cord.

Tue Mar 01 00:00:00 EST 2005

Activation of p38 mitogen-activated protein kinase in spinal microglia is a critical link in inflammation-induced spinal pain processing.

Journal of neurochemistry

Svensson CI,Marsala M,Westerlund A,Calcutt NA,Campana WM,Freshwater JD,Catalano R,Feng Y,Protter AA,Scott B,Yaksh TL

MA1-81606 was used in immunohistochemistry (frozen) to study the role of p38 mitogen-activated protein kinase in nociception.

Mon Sep 01 00:00:00 EDT 2003

Spinal p38 MAP kinase is necessary for NMDA-induced spinal PGE(2) release and thermal hyperalgesia.

Neuroreport

Svensson CI,Hua XY,Protter AA,Powell HC,Yaksh TL

MA1-81606 was used in immunohistochemistry to test if activation of spinal NMDA-receptor initiates activation of the p38 mitogen-activated protein kinase pathway and spinal release of prostaglandins and hyperalgesia.

Wed Jun 11 00:00:00 EDT 2003

LSF-1 may modulate the indirect allorecognition pathway to delay allograft rejection.

Transplant immunology

Vari F,Lord R,Goto S

MA1-81606 was used in flow cytometry to examine the role LSF-1 in liver regeneration after insult or injury.

Fri Nov 01 00:00:00 EST 2002

Propofol depressed neutrophil hydrogen peroxide production more than midazolam, whereas adhesion molecule expression was minimally affected by both anesthetics in rats with abdominal sepsis.

Anesthesia and analgesia

Inada T,Taniuchi S,Shingu K,Kobayashi Y,Fujisawa J,Nakao S

MA1-81606 was used in flow cytometry to examine the effect of anesthetic treatment on neutrophil function during sepsis.

Thu Feb 01 00:00:00 EST 2001

Cyclosporine facilitates chimeric and inhibits nonchimeric tolerance after posttransplant total lymphoid irradiation.

Transplantation

Lan F,Hayamizu K,Strober S

MA1-81606 was used in flow cytometry to discuss tolerance to ACI heart allografts using rats.

Sun Feb 27 00:00:00 EST 2000

In vivo reversal of amyloid-beta lesions in rat brain.

Journal of neuropathology and experimental neurology

Sigurdsson EM,Permanne B,Soto C,Wisniewski T,Frangione B

MA1-81606 was used in immunohistochemistry to investigate the effect of beta-sheet breaker treatment on disassembly of fibrillar amyloid deposits.

Sat Jan 01 00:00:00 EST 2000

Combined TUNEL and double immunofluorescent labeling for detection of apoptotic mononuclear phagocytes in autoimmune demyelinating disease.

Brain research. Brain research protocols

Ray SK,Schaecher KE,Shields DC,Hogan EL,Banik NL

MA1-81606 was used in immunocytochemistry to use TUNEL and an immunofluorescent labeling technique to determine the fate of cells in the central nervous system of mice with experimental allergic encephalomyelitis.

Sat Jul 01 00:00:00 EDT 2000

Comparison of chimeric acid and non-chimeric tolerance using posttransplant total lymphoid irradiation: cytokine expression and chronic rejection.

Transplantation

Hayamizu K,Lan F,Huie P,Sibley RK,Strober S

MA1-81606 was used in flow cytometry to study tolerance induction using heart allografts in Lewis rat.

Fri Oct 15 00:00:00 EDT 1999

Priming in the brain, an immunologically privileged organ, elicits anti-tumor immunity.

International journal of cancer

Fathallah-Shaykh HM,Gao W,Cho M,Herrera MA

MA1-81606 was used in immunohistochemistry (frozen) to report successful immunization against a central-nervous-system tumor.

Mon Jan 19 00:00:00 EST 1998

Monocyte-derived dendritic cell precursors facilitate tolerance to heart allografts after total lymphoid irradiation.

Transplantation

Hayamizu K,Huie P,Sibley RK,Strober S

MA1-81606 was used in flow cytometry to discuss factors that promote tolerance in a rat ACI heart allografts model.

Fri Nov 27 00:00:00 EST 1998

Donor blood monocytes but not T or B cells facilitate long-term allograft survival after total lymphoid irradiation.

Transplantation

Hayamizu K,Zeng D,Huie P,Garcia-Ojeda ME,Bloch DA,Fong L,Engleman EG,Sibley RK,Strober S

MA1-81606 was used in flow cytometry to determine the subset(s) of cells in donor blood that promote long-term allograft survival in rats.

Tue Sep 15 00:00:00 EDT 1998

Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies.

European journal of immunology

Tamatani T,Kotani M,Miyasaka M

MA1-81606 was used in flow cytometry and immunoprecipitation to characterize expression of rat CD11/CD18

Fri Mar 01 00:00:00 EST 1991

Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies.

European journal of immunology

Tamatani T,Kotani M,Miyasaka M

MA1-81606 was used in flow cytometry and immunoprecipitation to characterize expression of rat CD11/CD18

Fri Mar 01 00:00:00 EST 1991

Bioinformatics

Protein Aliases: CD11bc; CR3A; Integrin alpha M; integrin alpha-M; integrin alpha-X; integrin, alpha X; MAC-1; MAC1A; MGC117044; MO1A; OX-42; OX42; SLEB6

View more View less

Gene Aliases: Cd11b; RGD1561123

View more View less

Entrez Gene ID: (Rat) 25021, (Rat) 499271

View more View less

Function(s)

opsonin binding glycoprotein binding heparin binding heparan sulfate proteoglycan binding protein heterodimerization activity integrin

Process(es)

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion integrin-mediated signaling pathway ectodermal cell differentiation microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation heterotypic cell-cell adhesion defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD11b/c Monoclonal Antibody (OX-42)

CD11b/c Antibody (MA1-81606) in ICC/IF

Product Details

MA1-81606

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!