Invitrogen

CD11b/c Monoclonal Antibody (OX42), PE, eBioscience™

4 Published Figures

15 References

View all (16) CD11b/c antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

CD11b/c Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD11b/c Antibody (12-0110-82) in Flow

Staining of rat splenocytes with Anti-Rat Granulocyte Marker FITC (Product # 11-0570-82) and 0.06 µg of Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (left) or 0.06 µg of Anti-Rat CD11b/c PE (right). Total viable cells were used for analysis.

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Product Details

12-0110-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Rat

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OX42

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11150971

Product Specific Information

Description: This OX42 monoclonal antibody reacts with rat CD11b and CD11c. CD11b, also known as integrin alpha M or Mac-1, is a component of complement receptor 3 (CR3). CD11c, also known as integrin alpha X, is a component of complement receptor 4 (CR4). CD11b and CD11c are expressed on immune cells such as macrophages, monocytes, granulocytes, and dendritic cells. OX42 has also been shown to detect microglia in the brain, as well as cells of the liver and epidermis.

The OX42 antibody has been reported to inhibit complement-mediated rosette formation by granulocytes and macrophages. Moreover, studies demonstrate that OX42 can inhibit granulocyte aggregation.

Applications Reported: This OX42 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX42 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. CD11b is also a receptor for fibrogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% thymocytes and is commonly used as a microgial marker in nervous tissue. CD11c encodes the integrin alpha X chain protein. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Mutations affecting the CD11b/c gene lead to systemic lupus erythematosus 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Protective effect of HDACIs in improves survival and organ injury after CLP-induced sepsis.

Surgery open science

Niu K,Qu S,Yang L,Zhang H,Yuan J,Fan H,Li X,Tao K

12-0110-82 was used in Flow cytometry/Cell sorting to indicate that HDACI classes I and IIb and non-selective HDACI can significantly prolong survival.

Wed Mar 01 00:00:00 EST 2023

Atractylenolide III ameliorates spinal cord injury in rats by modulating microglial/macrophage polarization.

CNS neuroscience & therapeutics

Xue MT,Sheng WJ,Song X,Shi YJ,Geng ZJ,Shen L,Wang R,Lü HZ,Hu JG

12-0110-82 was used in flow cytometry to reveal a novel effect of ATL-III in the regulation of microglial/macrophage polarization and provides initial evidence that ATL-III has potential therapeutic benefits in SCI rats.

Fri Jul 01 00:00:00 EDT 2022

Negative pressure wound therapy improves bone regeneration by promoting osteogenic differentiation via the AMPK-ULK1-autophagy axis.

Autophagy

Zhang S,Xie Y,Yan F,Zhang Y,Yang Z,Chen Z,Zhao Y,Huang Z,Cai L,Deng Z

12-0110-82 was used in Flow cytometry/Cell sorting to identify a novel AMPK-ULK1-autophagy axis by which negative pressure promotes osteoblast differentiation of MSCs and bone regeneration.

Thu Sep 01 00:00:00 EDT 2022

Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats.

Molecular medicine reports

Feng S,Wang S,Sun S,Su H,Zhang L

Published figure using CD11b/c monoclonal antibody (Product # 12-0110-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose.

Frontiers in immunology

Steines L,Poth H,Schuster A,Amann K,Banas B,Bergler T

12-0110-82 was used in Flow Cytometry to conclude that Tfh:B cell crosstalk was controlled only by hiCNI treatment, preventing the development of DSA and ABMR.

Tue Sep 28 00:00:00 EDT 2021

Disruption of Tfh:B Cell Interactions Prevents Antibody-Mediated Rejection in a Kidney Transplant Model in Rats: Impact of Calcineurin Inhibitor Dose.

Frontiers in immunology

Steines L,Poth H,Schuster A,Amann K,Banas B,Bergler T

12-0110-82 was used in Flow Cytometry to conclude that Tfh:B cell crosstalk was controlled only by hiCNI treatment, preventing the development of DSA and ABMR.

Tue Sep 28 00:00:00 EDT 2021

Macrophage-Derived Inflammation Induces a Transcriptome Makeover in Mesenchymal Stromal Cells Enhancing Their Potential for Tissue Repair.

International journal of molecular sciences

Maldonado-Lasunción I,O'Neill N,Umland O,Verhaagen J,Oudega M

12-0110-82 was used in Flow cytometry/Cell sorting to investigate macrophage-derived inflammation as a means to condition mesenchymal stromal cell by comprehensively analyzing their transcriptome and secretome.

Thu Jan 14 00:00:00 EST 2021

Placenta‑derived mesenchymal stem cells ameliorate lipopolysaccharide‑induced inflammation in RAW264.7 cells and acute lung injury in rats.

Molecular medicine reports

Yuan W,Song HY,Xiong J,Jiang WL,Kang GJ,Huang J,Xie SP

12-0110 was used in Flow cytometry/Cell sorting to demonstrate placenta‚derived mesenchymal stem cells could be used as therapy for lipopolysaccharide (LPS)‚induced acute lung injury.

Sat Aug 01 00:00:00 EDT 2020

Cardioprotective Effects of Latifolin Against Doxorubicin-Induced Cardiotoxicity by Macrophage Polarization in Mice.

Journal of cardiovascular pharmacology

Zhang N,Shou B,Chen L,Lai X,Luo Y,Meng X,Liu R

12-0110 was used in Flow cytometry/Cell sorting to demonstrate that latifolin prevented DOX-induced cardiac dysfunction and produced macrophage polarization in mice challenged with latifolin.

Mon Jun 01 00:00:00 EDT 2020

Bifidobacteria shape host neural circuits during postnatal development by promoting synapse formation and microglial function.

Scientific reports

Luck B,Engevik MA,Ganesh BP,Lackey EP,Lin T,Balderas M,Major A,Runge J,Luna RA,Sillitoe RV,Versalovic J

12-0110 was used in Flow cytometry/Cell sorting to indicate that neonatal microbial colonization of the gut elicits concomitant effects on the host CNS, which promote the homeostatic developmental balance of neural connections during the postnatal time period.

Fri May 08 00:00:00 EDT 2020

Bifidobacteria shape host neural circuits during postnatal development by promoting synapse formation and microglial function.

Scientific reports

Luck B,Engevik MA,Ganesh BP,Lackey EP,Lin T,Balderas M,Major A,Runge J,Luna RA,Sillitoe RV,Versalovic J

Fri May 08 00:00:00 EDT 2020

Sodium bicarbonate loading limits tubular cast formation independent of glomerular injury and proteinuria in Dahl salt-sensitive rats.

Clinical science (London, England : 1979)

Ray SC,Patel B,Irsik DL,Sun J,Ocasio H,Crislip GR,Jin CH,Chen J,Baban B,Polichnowski AJ,O'Connor PM

12-0110-82 was used in Flow Cytometry to study whether NaHCO3 protects from the development of renal injury in Dahl salt-sensitive rats via luminal alkalization which limits the formation of tubular casts, which are a prominent pathological feature in this model.

Fri Jun 15 00:00:00 EDT 2018

Genetic deletion of microglial Panx1 attenuates morphine withdrawal, but not analgesic tolerance or hyperalgesia in mice.

Channels (Austin, Tex.)

Burma NE,Leduc-Pessah H,Trang T

12-0110-82 was used in Flow Cytometry to investigate whether microglial Panx1 contributes to opioid-induced hyperalgesia and opioid analgesic tolerance using mice with a tamoxifen-inducible deletion of microglial Panx1.

Sun Sep 03 00:00:00 EDT 2017

Chitosan hydrogel improves mesenchymal stem cell transplant survival and cardiac function following myocardial infarction in rats.

Experimental and therapeutic medicine

Xu B,Li Y,Deng B,Liu X,Wang L,Zhu QL

12-0110 was used in Flow cytometry/Cell sorting to evaluate the suitability of chitosan hydrogel, an injectable scaffold, to deliver mesenchymal stem cells into infarcted myocardium.

Wed Feb 01 00:00:00 EST 2017

Renal allograft rejection, lymphocyte infiltration, and de novo donor-specific antibodies in a novel model of non-adherence to immunosuppressive therapy.

BMC immunology

Kühne L,Jung B,Poth H,Schuster A,Wurm S,Ruemmele P,Banas B,Bergler T

12-0110 was used in Flow cytometry/Cell sorting to investigate the immunological mechanisms underlying chronic renal allograft rejection in a model of non-adherence to immunosuppressive therapy.

Tue Dec 19 00:00:00 EST 2017

Persistent inflammatory state after photoreceptor loss in an animal model of retinal degeneration.

Scientific reports

Noailles A,Maneu V,Campello L,Gómez-Vicente V,Lax P,Cuenca N

12-0110 was used in Flow cytometry/Cell sorting to assess longitudinal changes in microglia during retinal degeneration in a model of dominant retinitis pigmentosa.

Wed Sep 14 00:00:00 EDT 2016

Leucine-rich repeat kinase 2 deficiency is protective in rhabdomyolysis-induced kidney injury.

Human molecular genetics

Boddu R,Hull TD,Bolisetty S,Hu X,Moehle MS,Daher JP,Kamal AI,Joseph R,George JF,Agarwal A,Curtis LM,West AB

12-0110 was used in Flow cytometry/Cell sorting to highlight several novel physiological roles for leucine-rich repeat kinase 2 in disposition to kidney disease.

Wed Jul 15 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD11bc; CR3A; Integrin alpha M; integrin alpha-M; integrin alpha-X; integrin, alpha X; MAC-1; MAC1A; MGC117044; MO1A; OX-42; OX42; SLEB6

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Gene Aliases: Cd11b; RGD1561123

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Entrez Gene ID: (Rat) 25021, (Rat) 499271

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Function(s)

opsonin binding glycoprotein binding heparin binding heparan sulfate proteoglycan binding protein heterodimerization activity integrin

Process(es)

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion integrin-mediated signaling pathway ectodermal cell differentiation microglia development single organismal cell-cell adhesion neutrophil chemotaxis cellular extravasation activated T cell proliferation heterotypic cell-cell adhesion defense response to virus

Performance Guarantee

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CD11b/c Monoclonal Antibody (OX42), PE, eBioscience™

CD11b/c Antibody (12-0110-82) in Flow

Product Details

12-0110-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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