Invitrogen

CD120a (TNF Receptor I) Monoclonal Antibody (55R-170), Functional Grade, eBioscience™

CD120a (TNF Receptor I) Antibody in Functional Assay (Functional)

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CD120a (TNF Receptor I) Antibody (16-1202-81) in Functional

Anti-Mouse CD120a (TNF Receptor I) Functional Grade Purified inhibits the biological effects of recombinant mouse TNF alpha in a bioassay of TNF alpha induced cytotoxicity of the L929 cell line. Antibody concentration is in µg/mL.

CD120a (TNF Receptor I) Antibody in Neutralization (Neu)

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Product Details

16-1202-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

View 3 publications 3 publications

Neutralization (Neu)

Assay-Dependent

View 6 publications 6 publications

Functional Assay (Functional)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

55R-170

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1311128

Product Specific Information

Description: The 55R-170 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor I (TNFRI, TNFR-p55, TNFRSF1A, CD120a). TNFRI is expressed in a variety of cell types. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-alpha and TNF-beta. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release.

Applications Reported: The 55R-170 antibody has been reported for use in ELISA, neutralization of mouse TNFR I bioactivity and flow cytometric analysis.

Applications Tested: The Functional Grade Purified 55R-170 antibody has been tested by LAL assay to verify low endotoxin levels and in bioassay for neutralization of mouse TNFR I bioactivity.

The 55R-170 antibody at 25 µg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse TNF alpha in an assay of TNF alpha induced cytotoxicity of L929 cells. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the Best Protocols® section.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TNFR1 Contributes to Activation-Induced Cell Death of Pathological CD4⁺ T Lymphocytes During Ischemic Heart Failure.

JACC. Basic to translational science

Kumar V,Rosenzweig R,Asalla S,Nehra S,Prabhu SD,Bansal SS

Published figure using CD120a (TNF Receptor I) monoclonal antibody (Product # 16-1202-81) in Neutralization

Sat Oct 01 00:00:00 EDT 2022

Zika virus-induced TNF-α signaling dysregulates expression of neurologic genes associated with psychiatric disorders.

Journal of neuroinflammation

Kung PL,Chou TW,Lindman M,Chang NP,Estevez I,Buckley BD,Atkins C,Daniels BP

Published figure using CD120a (TNF Receptor I) monoclonal antibody (Product # 16-1202-81) in Neutralization

Sun Apr 24 00:00:00 EDT 2022

Neuronal TNFα, Not α-Syn, Underlies PDD-Like Disease Progression in IFNβ-KO Mice.

Annals of neurology

Villanueva EB,Tresse E,Liu Y,Duarte JN,Jimenez-Duran G,Ejlerskov P,Kretz O,Loreth D,Goldmann T,Prinz M,Issazadeh-Navikas S

Published figure using CD120a (TNF Receptor I) monoclonal antibody (Product # 16-1202-81) in Neutralization

Mon Nov 01 00:00:00 EDT 2021

TNF-α-dependent neuronal necroptosis regulated in Alzheimer's disease by coordination of RIPK1-p62 complex with autophagic UVRAG.

Theranostics

Xu C,Wu J,Wu Y,Ren Z,Yao Y,Chen G,Fang EF,Noh JH,Liu YU,Wei L,Chen X,Sima J

Published figure using CD120a (TNF Receptor I) monoclonal antibody (Product # 16-1202-81) in Neutralization

Mon Feb 07 00:00:00 EST 2022

Human CD8 T cells are susceptible to TNF-mediated activation-induced cell death.

Theranostics

Otano I,Alvarez M,Minute L,Ochoa MC,Migueliz I,Molina C,Azpilikueta A,de Andrea CE,Etxeberria I,Sanmamed MF,Teijeira Á,Berraondo P,Melero I

16-1202-81 was used in Flow Cytometry to investigate the direct role of TNF in activation-induced cell death of CD8 T lymphocytes.

Thu Apr 01 00:00:00 EDT 2021

Suppression of LPS-induced inflammatory responses by the hydroxyl groups of dexamethasone.

Oncotarget

Chuang TY,Cheng AJ,Chen IT,Lan TY,Huang IH,Shiau CW,Hsu CL,Liu YW,Chang ZF,Tseng PH,Kuo JC

16-1202 was used in Flow cytometry/Cell sorting to demonstrate that Dex signaling is involved in the control of innate immunity.

Tue Jul 25 00:00:00 EDT 2017

Preferential TNFα signaling via TNFR2 regulates epithelial injury and duct obstruction in experimental biliary atresia.

JCI insight

Shivakumar P,Mizuochi T,Mourya R,Gutta S,Yang L,Luo Z,Bezerra JA

16-1202 was used in Neutralization experiments to determine factors regulating the pathogenic mechanisms of biliary atresia.

Thu Mar 09 00:00:00 EST 2017

NF-κB transcriptional activation by TNFα requires phospholipase C, extracellular signal-regulated kinase 2 and poly(ADP-ribose) polymerase-1.

Journal of neuroinflammation

Vuong B,Hogan-Cann AD,Alano CC,Stevenson M,Chan WY,Anderson CM,Swanson RA,Kauppinen TM

16-1202 was used in Neutralization experiments to evaluate the mechanisms by which TNFα activates PARP-1 and PARP-1 mediates NF-κB activation.

Fri Dec 04 00:00:00 EST 2015

NF-κB transcriptional activation by TNFα requires phospholipase C, extracellular signal-regulated kinase 2 and poly(ADP-ribose) polymerase-1.

Journal of neuroinflammation

Vuong B,Hogan-Cann AD,Alano CC,Stevenson M,Chan WY,Anderson CM,Swanson RA,Kauppinen TM

16-1202 was used in Neutralization experiments to evaluate the mechanisms by which TNFα activates PARP-1 and PARP-1 mediates NF-κB activation.

Fri Dec 04 00:00:00 EST 2015

Quantifying crosstalk among interferon-γ, interleukin-12, and tumor necrosis factor signaling pathways within a TH1 cell model.

Science signaling

Klinke DJ,Cheng N,Chambers E

16-1202 was used in Flow cytometry/Cell sorting to illustrate how model-based inference can be used to better understand how cells process and act upon biochemical cues present in the tissue microenvironment.

Tue Apr 17 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD120a; CD120a antigen; MGC19588; n-smase activation assoc. factor; p55; p60; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TNF receptor alpha chain; TNF-alpha-R1; TNF-R1; TNF-RI; TNFR; TNFR I; TNFR-I; Tumor necrosis factor receptor; Tumor necrosis factor receptor 1; Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor type I

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Gene Aliases: CD120a; FPF; p55; p55-R; TNF-alphaR1; TNF-R; TNF-R-I; TNF-R1; TNF-R55; TNFalpha-R1; TNFAR; Tnfr-1; Tnfr-2; Tnfr1; TNFR60; TNFRI; TNFRp55; Tnfrsf1a

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UniProt ID: (Mouse) P25118

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Entrez Gene ID: (Mouse) 21937

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Function(s)

protease binding tumor necrosis factor-activated receptor activity protein binding protein complex binding tumor necrosis factor binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD120a (TNF Receptor I) Monoclonal Antibody (55R-170), Functional Grade, eBioscience™

CD120a (TNF Receptor I) Antibody (16-1202-81) in Functional

Product Details

16-1202-81

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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