Invitrogen

CD122 Monoclonal Antibody (5H4), PE, eBioscience™

1 Published Figure

10 References

View all (48) CD122 antibodies

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

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CD122 Antibody (12-1221-82) in Flow

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 µg of Anti-Mouse CD122 PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-1221-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

5H4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465833

Product Specific Information

Description: The 5H4 antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor beta chain. CD122 is expressed by NK cells and some T cells in the periphery and is upregulated by activation. CD122 associates with CD25 and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to CD122 is not inhibited by the 5H4 antibody.

Applications Reported: The 5H4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 5H4 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms. The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122. The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein. Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Diverse CD8 T Cell Responses to Viral Infection Revealed by the Collaborative Cross.

Cell reports

Martin MD,Sompallae R,Winborn CS,Harty JT,Badovinac VP

12-1221 was used in Flow cytometry/Cell sorting to advance the utility of the Collaborative Cross (CC) as a tool to analyze the immune response to viral infection.

Tue Apr 14 00:00:00 EDT 2020

Keap1-Nrf2 System Plays an Important Role in Invariant Natural Killer T Cell Development and Homeostasis.

Cell reports

Pyaram K,Kumar A,Kim YH,Noel S,Reddy SP,Rabb H,Chang CH

12-1221-82 was used in Flow Cytometry to report that T cellspecific deletion of Keap1 results in defective development and reduced numbers of invariant natural killer T (NKT) cells in the thymus and the peripheral organs in a cell-intrinsic manner.

Tue Apr 16 00:00:00 EDT 2019

Keap1-Nrf2 System Plays an Important Role in Invariant Natural Killer T Cell Development and Homeostasis.

Cell reports

Pyaram K,Kumar A,Kim YH,Noel S,Reddy SP,Rabb H,Chang CH

Tue Apr 16 00:00:00 EDT 2019

Roquin Suppresses the PI3K-mTOR Signaling Pathway to Inhibit T Helper Cell Differentiation and Conversion of Treg to Tfr Cells.

Immunity

Essig K,Hu D,Guimaraes JC,Alterauge D,Edelmann S,Raj T,Kranich J,Behrens G,Heiseke A,Floess S,Klein J,Maiser A,Marschall S,Hrabĕ de Angelis M,Leonhardt H,Calkhoven CF,Noessner E,Brocker T,Huehn J,Krug AB,Zavolan M,Baumjohann D,Heissmeyer V

12-1221-82 was used in Flow Cytometry to show Roquin-mediated control of PI3K-mTOR signaling prevents autoimmunity by restraining activation and differentiation of conventional T cells and specialization of Treg cells.

Tue Dec 19 00:00:00 EST 2017

Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells.

PLoS pathogens

Frenkel D,Zhang F,Guirnalda P,Haynes C,Bockstal V,Radwanska M,Magez S,Black SJ

12-1221 was used in Flow cytometry/Cell sorting to investigate the mechanism by which natural killer cells in Trypanosoma brucei infected mice kill B cells, suppress humoral immunity and induce early mortality.

Fri Jul 01 00:00:00 EDT 2016

IDO1 Deficiency Does Not Affect Disease in Mouse Models of Systemic Juvenile Idiopathic Arthritis and Secondary Hemophagocytic Lymphohistiocytosis.

PloS one

Put K,Brisse E,Avau A,Imbrechts M,Mitera T,Janssens R,Proost P,Fallarino F,Wouters CH,Matthys P

12-1221 was used in Flow cytometry/Cell sorting to unravel the role of IDO1, an immune-modulatory enzyme, in the pathogenesis of certain inflammatory diseases.

Mon Aug 08 00:00:00 EDT 2016

The ARNT-STAT3 axis regulates the differentiation of intestinal intraepithelial TCRαβ⁺CD8αα⁺ cells.

Nature communications

Nakajima K,Maekawa Y,Kataoka K,Ishifune C,Nishida J,Arimochi H,Kitamura A,Yoshimoto T,Tomita S,Nagahiro S,Yasutomo K

12-1221 was used in Flow cytometry/Cell sorting to demonstrate that the ARNT-STAT3 axis is a critical regulator of TCRαβ(+)CD8αα(+) intestinal intraepithelial T-cell development and differentiation.

Thu Jan 30 00:00:00 EST 2014

Host-derived interleukin-1alpha is important in determining the immunogenicity of 3-methylcholantrene tumor cells.

Journal of immunology (Baltimore, Md. : 1950)

Elkabets M,Krelin Y,Dotan S,Cerwenka A,Porgador A,Lichtenstein RG,White MR,Zoller M,Iwakura Y,Dinarello CA,Voronov E,Apte RN

12-1221 was used in Flow cytometry/Cell sorting to elucidate the involvement of IL-1 molecules in 3-MCA-induced carcinogenesis.

Wed Apr 15 00:00:00 EDT 2009

Gammadelta T lymphocyte homeostasis is negatively regulated by beta2-microglobulin.

Journal of immunology (Baltimore, Md. : 1950)

French JD,Roark CL,Born WK,O'Brien RL

12-1221 was used in Flow cytometry/Cell sorting to investigate the role of β2-microglobulin in regulating gamma-delta T lymphocyte homeostasis.

Sun Feb 15 00:00:00 EST 2009

Adjuvants targeting innate and adaptive immunity synergize to enhance tumor immunotherapy.

Proceedings of the National Academy of Sciences of the United States of America

Verdeil G,Marquardt K,Surh CD,Sherman LA

12-1221 was used in Flow cytometry/Cell sorting to study the combined use of two types of immune adjuvants that synergise to enhance tumour immunotherapy.

Tue Oct 28 00:00:00 EDT 2008

Presenilins regulate alphabeta T cell development by modulating TCR signaling.

The Journal of experimental medicine

Laky K,Fowlkes BJ

12-1221 was used in Flow cytometry/Cell sorting to suggest a model in which PS-dependent Notch signalling influences positive selection and the development of alphabeta T cells by modifying TCR signal transduction.

Mon Sep 03 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD122; High affinity IL-2 receptor subunit beta; IL-15 receptor beta chain; IL-2 receptor beta chain; IL-2 receptor subunit beta; IL-2/15 receptor-beta; IL-2R subunit beta; IL-2RB; Interleukin-2 receptor subunit beta; p70-75

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Gene Aliases: CD122; IL-15Rbeta; Il-2/15Rbeta; Il-2Rbeta; IL15Rbeta; Il2rb; p70

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UniProt ID: (Mouse) P16297

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Entrez Gene ID: (Mouse) 16185

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Function(s)

cytokine receptor activity interleukin-2 receptor activity interleukin-2 binding transmembrane signal receptor

Process(es)

cytokine-mediated signaling pathway natural killer cell activation interleukin-2-mediated signaling pathway negative regulation of apoptotic process

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CD122 Monoclonal Antibody (5H4), PE, eBioscience™

CD122 Antibody (12-1221-82) in Flow

Product Details

12-1221-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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