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Description: This TU27 monoclonal antibody reacts with human CD122, which is also known as interleukin-2 receptor beta (IL-2R beta). This receptor is expressed on NK cells, activated T cells, some B cells, monocytes, and T cell leukemias. CD122 can also be detected during early NK cell commitment. IL-2R beta associates with the IL-2R alpha (CD25) or IL-15R alpha and common gamma chain (CD132) to form the high affinity IL-2 and IL-15 receptors, respectively. By itself, this membrane receptor can bind IL-2, but with intermediate affinity. The binding of IL-2 results in internalization of the cytokine, tyrosine phosphorylation of IL-2Rbeta, and activation of signaling cascades such as the JAK/STAT pathway.
Applications Reported: This TU27 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TU27 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms. The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122. The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein. Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Protein Aliases: CD122; CD122 antigen; high affinity IL-2 receptor beta subunit; High affinity IL-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; interleukin 15 receptor, beta; interleukin 2 receptor, beta; Interleukin-15 receptor subunit beta; Interleukin-2 receptor subunit beta; p70-75; p75
Gene Aliases: CD122; IL15RB; IL2RB; P70-75
UniProt ID: (Human) P14784
Entrez Gene ID: (Human) 3560
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