Invitrogen

CD123 Monoclonal Antibody (6H6), Biotin, eBioscience™

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

6 Published Figures

14 References

View all (67) CD123 antibodies

Datasheet

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Protocols

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FIGURE: 1 / 8

CD123 Antibody (13-1239-82) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD11c PE and CD123 Biotin followed by APC conjugated streptavidin. As expected based on known relative expression patterns, CD123 clone 6H6 stains basophils (cyan), but not lymphocytes (pink), monocytes (orange) and granulocytes (blue).

CD123 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

CD123 Antibody — Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD11c PE and CD123 Biotin followed by APC conjugated streptavidin. As expected based on known relative expression patterns, CD123 clone 6H6 stains basophils (cyan), but not lymphocytes (pink), monocytes (orange) and granulocytes (blue). {RE}

Product Details

13-1239-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

6H6

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466586

Product Specific Information

Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

Applications Reported: The 6H6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 6H6 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and is a heterodimer with a unique alpha chain paired with the common beta (beta c or CDw131) subunit. IL-3Ra is responsible for the transmission of signal of Interleukin-3. IL-3Ra and IL-3Rb chain heterodimerization is induced by Interleukin-3, which is required for receptor activation but not high affinity binding. IL-3Ra is also reported that IL-3Ra subunits are overexpressed on acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells and are thus potential targets for novel therapeutic agents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population Discovery.

Frontiers in immunology

Baumgaertner P,Sankar M,Herrera F,Benedetti F,Barras D,Thierry AC,Dangaj D,Kandalaft LE,Coukos G,Xenarios I,Guex N,Harari A

Published figure using CD123 monoclonal antibody (Product # 13-1239-82) in Flow Cytometry

Tue Sep 21 00:00:00 EDT 2021

Multiplexed Imaging for Immune Profiling on Human FFPE Material.

Methods in molecular biology (Clifton, N.J.)

Mezheyeuski A,Strell C

13-1239-82 was used in Sample Preparation to describe the pipeline for multiplex immunohistochemical staining, multispectral image acquisition, and analysis for use on human formalin fixed paraffin embedded tissues.

Wed Sep 01 00:00:00 EDT 2021

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD123 monoclonal antibody (Product # 13-1239-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults.

Frontiers in immunology

Ben-Othman R,Cai B,Liu AC,Varankovich N,He D,Blimkie TM,Lee AH,Gill EE,Novotny M,Aevermann B,Drissler S,Shannon CP,McCann S,Marty K,Bjornson G,Edgar RD,Lin DTS,Gladish N,Maclsaac J,Amenyogbe N,Chan Q,Llibre A,Collin J,Landais E,Le K,Reiss SM,Koff WC,Havenar-Daughton C,Heran M,Sangha B,Walt D,Krajden M,Crotty S,Sok D,Briney B,Burton DR,Duffy D,Foster LJ,Mohn WW,Kobor MS,Tebbutt SJ,Brinkman RR,Scheuermann RH,Hancock REW,Kollmann TR,Sadarangani M

Published figure using CD123 monoclonal antibody (Product # 13-1239-82) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro.

Frontiers in immunology

Bastian OW,Croes M,Alblas J,Koenderman L,Leenen LPH,Blokhuis TJ

Published figure using CD123 monoclonal antibody (Product # 13-1239-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.

Cell

Lim AI,Li Y,Lopez-Lastra S,Stadhouders R,Paul F,Casrouge A,Serafini N,Puel A,Bustamante J,Surace L,Masse-Ranson G,David E,Strick-Marchand H,Le Bourhis L,Cocchi R,Topazio D,Graziano P,Muscarella LA,Rogge L,Norel X,Sallenave JM,Allez M,Graf T,Hendriks RW,Casanova JL,Amit I,Yssel H,Di Santo JP

13-1239 was used in Flow cytometry/Cell sorting to identify circulating and tissue ILC precursors in humans that fail to express the transcription factors and cytokine outputs of mature ILCs but have these signature loci in an epigenetically poised configuration.

Thu Mar 09 00:00:00 EST 2017

Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.

PLoS pathogens

Ataide MA,Andrade WA,Zamboni DS,Wang D,Souza Mdo C,Franklin BS,Elian S,Martins FS,Pereira D,Reed G,Fitzgerald KA,Golenbock DT,Gazzinelli RT

Published figure using CD123 monoclonal antibody (Product # 13-1239-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Frailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation.

PloS one

Compté N,Zouaoui Boudjeltia K,Vanhaeverbeek M,De Breucker S,Tassignon J,Trelcat A,Pepersack T,Goriely S

Published figure using CD123 monoclonal antibody (Product # 13-1239-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2014

PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.

Blood

van de Laar L,van den Bosch A,Boonstra A,Binda RS,Buitenhuis M,Janssen HL,Coffer PJ,Woltman AM

13-1239 was used in Flow cytometry/Cell sorting to investigate the role of the PI3K-PKB-mTOR axis in human plasmacytoid dendritic cell development.

Thu Dec 13 00:00:00 EST 2012

Irreversible loss of pDCs by apoptosis during early HIV infection may be a critical determinant of immune dysfunction.

Viral immunology

Meera S,Madhuri T,Manisha G,Ramesh P

Published figure using CD123 monoclonal antibody (Product # 13-1239-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2010

Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.

Nature immunology

Xu W,He B,Chiu A,Chadburn A,Shan M,Buldys M,Ding A,Knowles DM,Santini PA,Cerutti A

Thu Mar 01 00:00:00 EST 2007

Local activation of the innate immune system in Buruli ulcer lesions.

The Journal of investigative dermatology

Peduzzi E,Groeper C,Schütte D,Zajac P,Rondini S,Mensah-Quainoo E,Spagnoli GC,Pluschke G,Daubenberger CA

Thu Mar 01 00:00:00 EST 2007

CD56-positive haematological neoplasms of the skin: a multicentre study of the Cutaneous Lymphoma Project Group of the European Organisation for Research and Treatment of Cancer.

Journal of clinical pathology

Assaf C,Gellrich S,Whittaker S,Robson A,Cerroni L,Massone C,Kerl H,Rose C,Chott A,Chimenti S,Hallermann C,Petrella T,Wechsler J,Bagot M,Hummel M,Bullani-Kerl K,Bekkenk MW,Kempf W,Meijer CJ,Willemze R,Sterry W

Published figure using CD123 monoclonal antibody (Product # 13-1239-82) in Immunohistochemistry

Sat Sep 01 00:00:00 EDT 2007

Expression of the plasmacytoid dendritic cell marker BDCA-2 supports a spectrum of maturation among CD4+ CD56+ hematodermic neoplasms.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Jaye DL,Geigerman CM,Herling M,Eastburn K,Waller EK,Jones D

Fri Dec 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD123; CD123 antigen; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; interleukin 3 receptor, alpha (low affinity); Interleukin-3 receptor subunit alpha

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Gene Aliases: CD123; hIL-3Ra; IL3R; IL3RA; IL3RAY; IL3RX; IL3RY

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UniProt ID: (Human) P26951

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Entrez Gene ID: (Human) 3563

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Function(s)

protein tyrosine kinase activity interleukin-3 receptor activity Ras guanyl-nucleotide exchange factor activity transmembrane signal receptor

Process(es)

MAPK cascade peptidyl-tyrosine phosphorylation cellular response to interleukin-3 interleukin-3-mediated signaling pathway positive regulation of GTPase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD123 Monoclonal Antibody (6H6), Biotin, eBioscience™

CD123 Antibody (13-1239-82) in Flow

Product Details

13-1239-82

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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