Invitrogen

CD123 Monoclonal Antibody (6H6), PerCP-Cyanine5.5, eBioscience™

5 Published Figures

19 References

View all (67) CD123 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

FIGURE: 1 / 6

CD123 Antibody (45-1239-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD85g (ILT7) APC (Product # 17-5179-42) and Mouse IgG1 K Isotype Control PerCP-Cyanine5-5 (Product # 45-4714-82) (left) or Anti-Human CD123 PerCP-Cyanine5-5 (right). Cells in the monocyte gate were used for analysis.

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Product Details

45-1239-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

6H6

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10718981

Product Specific Information

Description: The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

Applications Reported: This 6H6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 6H6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and is a heterodimer with a unique alpha chain paired with the common beta (beta c or CDw131) subunit. IL-3Ra is responsible for the transmission of signal of Interleukin-3. IL-3Ra and IL-3Rb chain heterodimerization is induced by Interleukin-3, which is required for receptor activation but not high affinity binding. IL-3Ra is also reported that IL-3Ra subunits are overexpressed on acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells and are thus potential targets for novel therapeutic agents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population Discovery.

Frontiers in immunology

Baumgaertner P,Sankar M,Herrera F,Benedetti F,Barras D,Thierry AC,Dangaj D,Kandalaft LE,Coukos G,Xenarios I,Guex N,Harari A

45-1239 was used in Flow cytometry/Cell sorting to perform longitudinal flow cytometry analyses to identify multiple distinct cell populations in circulating whole blood from cancer patients treated with radiotherapy in a clinical trial.

Tue Sep 21 00:00:00 EDT 2021

Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population Discovery.

Frontiers in immunology

Baumgaertner P,Sankar M,Herrera F,Benedetti F,Barras D,Thierry AC,Dangaj D,Kandalaft LE,Coukos G,Xenarios I,Guex N,Harari A

Tue Sep 21 00:00:00 EDT 2021

A Personalized Neoantigen Vaccine in Combination with Platinum-Based Chemotherapy Induces a T-Cell Response Coinciding with a Complete Response in Endometrial Carcinoma.

Cancers

Harari A,Sarivalasis A,de Jonge K,Thierry AC,Huber F,Boudousquie C,Rossier L,Orcurto A,Imbimbo M,Baumgaertner P,Bassani-Sternberg M,Kandalaft LE

45-1239-42 was used in Flow Cytometry to show the feasibility of generating a personalized dendritic cell vaccine pulsed with peptide neoantigens in a patient with pMMR, p53-mutated, and serous endometrial adenocarcinoma.

Thu Nov 18 00:00:00 EST 2021

Human Mast Cell Proteome Reveals Unique Lineage, Putative Functions, and Structural Basis for Cell Ablation.

Immunity

Plum T,Wang X,Rettel M,Krijgsveld J,Feyerabend TB,Rodewald HR

45-1239 was used in Flow cytometry/Cell sorting to identify a mast-cell-specific proteome signature, indicative of a unique lineage, only distantly related to other immune cell types, including innate immune cells.

Tue Feb 18 00:00:00 EST 2020

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD123 monoclonal antibody (Product # 45-1239-42) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults.

Frontiers in immunology

Ben-Othman R,Cai B,Liu AC,Varankovich N,He D,Blimkie TM,Lee AH,Gill EE,Novotny M,Aevermann B,Drissler S,Shannon CP,McCann S,Marty K,Bjornson G,Edgar RD,Lin DTS,Gladish N,Maclsaac J,Amenyogbe N,Chan Q,Llibre A,Collin J,Landais E,Le K,Reiss SM,Koff WC,Havenar-Daughton C,Heran M,Sangha B,Walt D,Krajden M,Crotty S,Sok D,Briney B,Burton DR,Duffy D,Foster LJ,Mohn WW,Kobor MS,Tebbutt SJ,Brinkman RR,Scheuermann RH,Hancock REW,Kollmann TR,Sadarangani M

Published figure using CD123 monoclonal antibody (Product # 45-1239-42) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Resistance of KIR Ligand-Missing Leukocytes to NK Cells In Vivo in Patients with Acquired Aplastic Anemia.

ImmunoHorizons

Nguyen MAT,Hosokawa K,Yoroidaka T,Maruyama H,Espinoza JL,Elbadry MI,Mohiuddin M,Tanabe M,Katagiri T,Nakagawa N,Chonabayashi K,Yoshida Y,Arima N,Kashiwase K,Saji H,Ogawa S,Nakao S

45-1239 was used in Flow cytometry/Cell sorting to suggest that human NK cells are able to suppress the expansion of KIR-L(-) leukocytes but are unable to eliminate them partly due to the lack of activating KIRs on NK cells and the low HLA-F expression level on hematopoietic stem cells in AA patients.

Fri Jul 17 00:00:00 EDT 2020

50-Gy Stereotactic Body Radiation Therapy to the Dominant Intraprostatic Nodule: Results From a Phase 1a/b Trial.

International journal of radiation oncology, biology, physics

Herrera FG,Valerio M,Berthold D,Tawadros T,Meuwly JY,Vallet V,Baumgartner P,Thierry AC,De Bari B,Jichlinski P,Kandalaft L,Coukos G,Harari A,Bourhis J

45-1239-42 was used in Flow Cytometry to show stereotactic irradiation induced antigen-specific immune responses in a subset of patients.

Fri Feb 01 00:00:00 EST 2019

Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro.

Frontiers in immunology

Bastian OW,Croes M,Alblas J,Koenderman L,Leenen LPH,Blokhuis TJ

Published figure using CD123 monoclonal antibody (Product # 45-1239-42) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

Human Blood-Circulating Basophils Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells.

Journal of virology

Jiang AP,Jiang JF,Guo MG,Jin YM,Li YY,Wang JH

45-1239 was used in Flow cytometry/Cell sorting to investigate the role of human granulocytes in the dissemination of HIV-1.

Sat Aug 01 00:00:00 EDT 2015

Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells.

Journal of virology

Jiang AP,Jiang JF,Wei JF,Guo MG,Qin Y,Guo QQ,Ma L,Liu BC,Wang X,Veazey RS,Ding YB,Wang JH

45-1239 was used in Flow cytometry/Cell sorting to investigate the role of mast cells in HIV-1 infection.

Wed Dec 30 00:00:00 EST 2015

Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.

PLoS pathogens

Ataide MA,Andrade WA,Zamboni DS,Wang D,Souza Mdo C,Franklin BS,Elian S,Martins FS,Pereira D,Reed G,Fitzgerald KA,Golenbock DT,Gazzinelli RT

Published figure using CD123 monoclonal antibody (Product # 45-1239-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Dendritic cell maturation and survival are differentially regulated by TNFR1 and TNFR2.

Journal of immunology (Baltimore, Md. : 1950)

Maney NJ,Reynolds G,Krippner-Heidenreich A,Hilkens CMU

45-1239 was used in Flow cytometry/Cell sorting to show that TNFR1, but not TNFR2, signaling induces dendritic cell maturation, whereas survival can be mediated independently through both TNFR in humans.

Sat Nov 15 00:00:00 EST 2014

Frailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation.

PloS one

Compté N,Zouaoui Boudjeltia K,Vanhaeverbeek M,De Breucker S,Tassignon J,Trelcat A,Pepersack T,Goriely S

Published figure using CD123 monoclonal antibody (Product # 45-1239-42) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2014

Multiple sclerosis: modulation of toll-like receptor (TLR) expression by interferon-β includes upregulation of TLR7 in plasmacytoid dendritic cells.

PloS one

Derkow K,Bauer JM,Hecker M,Paap BK,Thamilarasan M,Koczan D,Schott E,Deuschle K,Bellmann-Strobl J,Paul F,Zettl UK,Ruprecht K,Lehnardt S

45-1239 was used in Flow cytometry/Cell sorting to investigate the mechanism of action of interferon-β as a treatment for MS, showing that it upregulates TLR7 expression in plasmacytoid dendritic cells.

Thu Mar 06 00:00:00 EST 2014

Irreversible loss of pDCs by apoptosis during early HIV infection may be a critical determinant of immune dysfunction.

Viral immunology

Meera S,Madhuri T,Manisha G,Ramesh P

Published figure using CD123 monoclonal antibody (Product # 45-1239-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2010

Epithelial cells trigger frontline immunoglobulin class switching through a pathway regulated by the inhibitor SLPI.

Nature immunology

Xu W,He B,Chiu A,Chadburn A,Shan M,Buldys M,Ding A,Knowles DM,Santini PA,Cerutti A

Thu Mar 01 00:00:00 EST 2007

Local activation of the innate immune system in Buruli ulcer lesions.

The Journal of investigative dermatology

Peduzzi E,Groeper C,Schütte D,Zajac P,Rondini S,Mensah-Quainoo E,Spagnoli GC,Pluschke G,Daubenberger CA

Thu Mar 01 00:00:00 EST 2007

CD56-positive haematological neoplasms of the skin: a multicentre study of the Cutaneous Lymphoma Project Group of the European Organisation for Research and Treatment of Cancer.

Journal of clinical pathology

Assaf C,Gellrich S,Whittaker S,Robson A,Cerroni L,Massone C,Kerl H,Rose C,Chott A,Chimenti S,Hallermann C,Petrella T,Wechsler J,Bagot M,Hummel M,Bullani-Kerl K,Bekkenk MW,Kempf W,Meijer CJ,Willemze R,Sterry W

Sat Sep 01 00:00:00 EDT 2007

Expression of the plasmacytoid dendritic cell marker BDCA-2 supports a spectrum of maturation among CD4+ CD56+ hematodermic neoplasms.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Jaye DL,Geigerman CM,Herling M,Eastburn K,Waller EK,Jones D

Fri Dec 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD123; CD123 antigen; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; interleukin 3 receptor, alpha (low affinity); Interleukin-3 receptor subunit alpha

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Gene Aliases: CD123; hIL-3Ra; IL3R; IL3RA; IL3RAY; IL3RX; IL3RY

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UniProt ID: (Human) P26951

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Entrez Gene ID: (Human) 3563

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Function(s)

protein tyrosine kinase activity interleukin-3 receptor activity Ras guanyl-nucleotide exchange factor activity transmembrane signal receptor

Process(es)

MAPK cascade peptidyl-tyrosine phosphorylation cellular response to interleukin-3 interleukin-3-mediated signaling pathway positive regulation of GTPase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD123 Monoclonal Antibody (6H6), PerCP-Cyanine5.5, eBioscience™

CD123 Antibody (45-1239-42) in Flow

Product Details

45-1239-42

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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