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Invitrogen
CD135 (Flt3) Monoclonal Antibody (BV10A4H2), PE, eBioscience™
Product Details
12-1357-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
BV10A4H2
Immunogen
BV-173 leukemic cell line
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10596974
Product Specific Information
Description: The BV10A4H2 monoclonal antibody reacts with Human CD135, also known as Flk2 or Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by multipotential progenitor cells including primitive B cell and myelomonocytic progenitors in fetal liver and adult bone marrow.
Applications Reported: The BV10A4H2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This BV10A4H2 antibody has been pre-titrated and tested by flow cytometric analysis of REH cell line. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK-2; FLT-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; Stem cell tyrosine kinase 1; STK-1
Gene Aliases: CD135; FLK-2; FLK2; FLT3; STK1
UniProt ID: (Human) P36888
Entrez Gene ID: (Human) 2322
leukocyte homeostasis
myeloid progenitor cell differentiation
pro-B cell differentiation
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of cell proliferation
response to organonitrogen compound
positive regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
hemopoiesis
B cell differentiation
organ regeneration
common myeloid progenitor cell proliferation
vascular endothelial growth factor signaling pathway
positive regulation of tyrosine phosphorylation of STAT protein
regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
positive regulation of phosphatidylinositol 3-kinase activity
lymphocyte proliferation
protein autophosphorylation
cellular response to cytokine stimulus
cellular response to glucocorticoid stimulus
dendritic cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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