Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (5G11), eBioscience™
FIGURE: 1 / 1
CD137 Ligand (4-1BB Ligand) Antibody (14-9056-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human lung tissue using 1 µg/mL of Mouse IgG2a K Isotype Control Purified (left) or 1 µg/mL of Anti-Human CD137 Ligand (4-1BB Ligand) Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.
Product Details
14-9056-82
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
5G11
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2662859
Product Specific Information
This 5G11 monoclonal antibody recognizes human CD137 ligand also known as 4-1BB ligand, TNFSF9, or CDw137L. CD137 ligand is a member of the TNF superfamily, and is a type II membrane protein. It is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation of these cells. Its receptor, CD137 (4-1BB) is found on a variety of cells, including inflamed endothelial cells, where it enhances extravasation of CD137 ligand-expressing monocytes. Activated T cells also express CD137, and engagement with CD137 ligand enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137 ligand is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but not on Hodgkin lymphoma and T-cell lymphoma).
This 5G11 antibody has been tested by immunohistochemical staining of formalin-fixed paraffin embedded human tissue using low pH antigen retreival and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. CD137L is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but it is lacking on Hodgkin lymphoma and T-cell lymphoma). CD137L is also expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 4 1 BB Ligand; 4 1 BBL; 4-1BB ligand; 4-1BBL; 41BB Ligand; 41BBL; CD 137 Ligand; CD 137L; CD137 Ligand; CDw137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand; TNFRSF9; tumor necrosis factor (ligand) superfamily member 9; tumor necrosis factor (ligand) superfamily, member 9; tumor necrosis factor ligand 5A; Tumor necrosis factor ligand superfamily member 9
Gene Aliases: 4-1BB-L; CD137L; TNFSF9; TNLG5A
UniProt ID: (Human) P41273
Entrez Gene ID: (Human) 8744
apoptotic process
immune response
signal transduction
cell-cell signaling
cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of activated T cell proliferation
myeloid dendritic cell differentiation
positive regulation of cytotoxic T cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support