Invitrogen

CD138 Polyclonal Antibody

10 Published Figures

20 References

View all (80) CD138 (Syndecan-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)
Published Figures (10)

CD138 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 17

CD138 Antibody (36-2900) in IHC (P)

Immunohistochemistry analysis of Syndecan-1/CD138 showing staining in the cytoplasm and membrane of paraffin-embedded human small intestinal tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol ... View More

CD138 Antibody in Immunohistochemistry (IHC)

CD138 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

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Product Details

36-2900

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

ELISA (ELISA)

0.1-1 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of the human Syndecan-1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533248

Product Specific Information

This antibody reacts with the human Syndecan-1 (CD38) protein. On western blots, a single band at ~90 kDa representing the glycosylated form of CD138; occasionally a band of ~31 kDa representing the un-glycosylated form of CD138 may be observed. Reactivity has been confirmed with human T47D, SW-480, and PC-3 cell lysates by western blotting and with formalin-fixed, paraffin-embedded (FFPE) human gastric cancer and breast cancer tissues, and mouse and rat stomach, small bowel, and skin tissues by immunohistochemistry. For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining.

Target Information

CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Profiling of extracellular vesicles of metastatic urothelial cancer patients to discover protein signatures related to treatment outcome.

Molecular oncology

Viktorsson K,Hååg P,Shah CH,Franzén B,Arapi V,Holmsten K,Sandström P,Lewensohn R,Ullén A

Published figure using CD138 polyclonal antibody (Product # 36-2900) in Western Blot

Sat Oct 01 00:00:00 EDT 2022

Unusual bilateral kidney and duodenal plasmablastic lymphoma presentation in an elderly patient: A case report.

Molecular and clinical oncology

Liu YC,Su YT,Huang CK,Tsai YC,Chen YC,Li PF

36-2900 was used in Immunohistochemistry to describe the case of a 71-year-old man who was admitted to the emergency department with a 7-day history of progressive left flank pain and tarry stool.

Fri Jul 01 00:00:00 EDT 2022

Balance between immunoregulatory B cells and plasma cells drives pancreatic tumor immunity.

Cell reports. Medicine

Mirlekar B,Wang Y,Li S,Zhou M,Entwistle S,De Buysscher T,Morrison A,Herrera G,Harris C,Vincent BG,Ting JP,Rashid N,Kim WY,Yeh JJ,Pylayeva-Gupta Y

36-2900 was used in Immunohistochemistry to argue that B cell effector dysfunction in cancer can be due to an active systemic suppression program that can be targeted to synergize with T cell-directed immunotherapy.

Tue Sep 20 00:00:00 EDT 2022

O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination.

Journal of experimental & clinical cancer research : CR

Samart P,Luanpitpong S,Rojanasakul Y,Issaragrisil S

36-2900 was used in Immunohistochemistry (Paraffin) to unveil a novel regulatory mechanism of MM cell motility via Ca2+ influx/O-GlcNAcylation axis that directly targets integrin α4 and integrin β7, providing mechanistic insights into the pathogenesis and progression of MM and demonstrating potential predictive biomarkers and therapeutic targets for advanced MM.

Tue Mar 16 00:00:00 EDT 2021

O-GlcNAcylation homeostasis controlled by calcium influx channels regulates multiple myeloma dissemination.

Journal of experimental & clinical cancer research : CR

Samart P,Luanpitpong S,Rojanasakul Y,Issaragrisil S

Tue Mar 16 00:00:00 EDT 2021

Polarized Secretion of APRIL by the Tonsil Epithelium Upon Toll-Like Receptor Stimulation.

Frontiers in immunology

Sturm N,Quinterot M,Guyot JP,Righini C,Daamen WF,van Kuppevelt TH,Huard B

Published figure using CD138 polyclonal antibody (Product # 36-2900) in Immunohistochemistry

Mon Dec 20 00:00:00 EST 2021

Polarized Secretion of APRIL by the Tonsil Epithelium Upon Toll-Like Receptor Stimulation.

Frontiers in immunology

Sturm N,Quinterot M,Guyot JP,Righini C,Daamen WF,van Kuppevelt TH,Huard B

Published figure using CD138 polyclonal antibody (Product # 36-2900) in Immunohistochemistry

Mon Dec 20 00:00:00 EST 2021

Transmembrane Protein TMEM230, a Target of Glioblastoma Therapy.

Frontiers in cellular neuroscience

Cocola C,Magnaghi V,Abeni E,Pelucchi P,Martino V,Vilardo L,Piscitelli E,Consiglio A,Grillo G,Mosca E,Gualtierotti R,Mazzaccaro D,La Sala G,Di Pietro C,Palizban M,Liuni S,DePedro G,Morara S,Nano G,Kehler J,Greve B,Noghero A,Marazziti D,Bussolino F,Bellipanni G,D'Agnano I,Götte M,Zucchi I,Reinbold R

Published figure using CD138 polyclonal antibody (Product # 36-2900) in Western Blot

Tue Dec 07 00:00:00 EST 2021

SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines.

International journal of molecular medicine

Farfán N,Orellana-Serradell O,Herrera D,Chrzanowsky D,Cubillos P,Marín G,Antonio García De Herreros A,Castellón EA,Contreras HR

Published figure using CD138 polyclonal antibody (Product # 36-2900) in Immunocytochemistry

Wed Apr 01 00:00:00 EDT 2020

SNAIL expression correlates with the translocation of syndecan‑1 intracellular domain into the nucleus in prostate cancer cell lines.

International journal of molecular medicine

Farfán N,Orellana-Serradell O,Herrera D,Chrzanowsky D,Cubillos P,Marín G,Antonio García De Herreros A,Castellón EA,Contreras HR

Published figure using CD138 polyclonal antibody (Product # 36-2900) in Immunocytochemistry

Wed Apr 01 00:00:00 EDT 2020

Skin-specific antibodies neutralizing mycolactone toxin during the spontaneous healing of Mycobacterium ulcerans infection.

Science advances

Foulon M,Pouchin A,Manry J,Khater F,Robbe-Saule M,Durand A,Esnault L,Delneste Y,Jeannin P,Saint-André JP,Croué A,Altare F,Abel L,Alcaïs A,Marion E

Published figure using CD138 polyclonal antibody (Product # 36-2900) in Immunohistochemistry

Sat Feb 01 00:00:00 EST 2020

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts.

Journal of visualized experiments : JoVE

van de Vlekkert D,Qiu X,Annunziata I,d'Azzo A

36-2900 was used in Western Blotting to present a protocol for establishing cultures of primary fibroblasts isolated from adult mouse skeletal muscles, followed by purification and characterization of exosomes from the culture media of these cells.

Sat May 16 00:00:00 EDT 2020

Isolation, Purification and Characterization of Exosomes fromFibroblast Cultures of Skeletal Muscle.

Bio-protocol

Van De Vlekkert D,Qiu X,Annunziata I,d'Azzo A

36-2900 was used in Western Blot to provide a protocol based on differential ultracentrifugation and sucrose density gradients tailored for the isolation of crude and ultra-pure exosomes from primary fibroblast cultures derived from adult mouse skeletal muscle.

Sun Apr 05 00:00:00 EDT 2020

Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases.

Communications biology

Atsumi S,Katoh H,Komura D,Hashimoto I,Furuya G,Koda H,Konishi H,Suzuki R,Yamamoto A,Yuba S,Abe H,Rino Y,Oshima T,Ushiku T,Fukayama M,Seto Y,Ishikawa S

36-2900 was used in Immunohistochemistry to show that B-cell repertoire sequencing of 102 clinical gastric cancers and molecular biological analyses unexpectedly reveal that the major humoral cancer antigens are not case-specific neo-antigens but are rather commonly identified as ribonucleoproteins (RNPs) in the focal adhesion complex.

Fri Oct 16 00:00:00 EDT 2020

Mapping the Interactome of the Nuclear Heparan Sulfate Proteoglycan Syndecan-1 in Mesothelioma Cells.

Biomolecules

Kumar-Singh A,Shrinet J,Parniewska MM,Fuxe J,Dobra K,Hjerpe A

36-2900 was used in Immunoprecipitation to study the role of nuclear Syndecan-1 (SDC1) in Mesothelioma Cells.

Sat Jul 11 00:00:00 EDT 2020

Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis.

Science advances

van de Vlekkert D,Demmers J,Nguyen XX,Campos Y,Machado E,Annunziata I,Hu H,Gomero E,Qiu X,Bongiovanni A,Feghali-Bostwick CA,d'Azzo A

36-2900 was used in Western Blotting to reveal an unexpected exosome-mediated signaling pathway downstream of NEU1 deficiency that propagates a fibrotic disease and could be implicated in idiopathic forms of fibrosis in humans.

Mon Jul 01 00:00:00 EDT 2019

Neurotoxic reactive astrocytes are induced by activated microglia.

Nature

Liddelow SA,Guttenplan KA,Clarke LE,Bennett FC,Bohlen CJ,Schirmer L,Bennett ML,Münch AE,Chung WS,Peterson TC,Wilton DK,Frouin A,Napier BA,Panicker N,Kumar M,Buckwalter MS,Rowitch DH,Dawson VL,Dawson TM,Stevens B,Barres BA

36-2900 was used in western blot to identify an astrocyte subtype induced by activated microglia

Thu Jan 26 00:00:00 EST 2017

Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from polyposis initiation.

International journal of cancer

Allaire JM,Roy SA,Ouellet C,Lemieux É,Jones C,Paquet M,Boudreau F,Perreault N

36-2900 was used in western blot to determine how Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from initiation of polyposis

Wed Jun 01 00:00:00 EDT 2016

A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness.

PloS one

Grau L,Luque-Garcia JL,González-Peramato P,Theodorescu D,Palou J,Fernandez-Gomez JM,Sánchez-Carbayo M

36-2900 was used in Western Blotting to perform a proteomic analysis comparing an invasive T24 and an aggressive metastatic derived T24T bladder cancer cell line.

Tue Jul 02 00:00:00 EDT 2013

Syndecan 1 plays a novel role in enteral glutamine's gut-protective effects of the postischemic gut.

Shock (Augusta, Ga.)

Peng Z,Ban K,Sen A,Grill R,Park P,Costantini TW,Kozar R

36-2900 was used in Western Blotting to study the effects of loss of epithelial syndecan 1 on the gut-protective effects of enteral glutamine.

Sun Jul 01 00:00:00 EDT 2012

Heparan sulfate proteoglycans are required for cellular binding of the hepatitis E virus ORF2 capsid protein and for viral infection.

Journal of virology

Kalia M,Chandra V,Rahman SA,Sehgal D,Jameel S

Tue Dec 01 00:00:00 EST 2009

Primary human glomerular endothelial cells produce proteoglycans, and puromycin affects their posttranslational modification.

American journal of physiology. Renal physiology

Björnson A,Moses J,Ingemansson A,Haraldsson B,Sörensson J

Fri Apr 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: CD antigen 138; CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; sCD138; soluble CD138; SYND1; syndecan proteoglycan 1; Syndecan-1; Syndecan1; synstatin

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Gene Aliases: AA408134; AA409076; CD138; HSPG; SDC; SDC1; Sstn; syn-1; Synd; Synd-1; SYND1; SYNDECA; Syndecan

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UniProt ID: (Human) P18827, (Mouse) P18828, (Rat) P26260

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Entrez Gene ID: (Human) 6382, (Mouse) 20969, (Rat) 25216

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Function(s)

glycoprotein binding protein binding protein C-terminus binding extracellular matrix binding transmembrane signal receptor

Process(es)

retinoid metabolic process ureteric bud development glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process inflammatory response response to toxic substance cell migration glycosaminoglycan metabolic process wound healing lipoprotein metabolic process odontogenesis response to hydrogen peroxide myoblast development response to glucocorticoid response to cAMP response to calcium ion striated muscle cell development Sertoli cell development canonical Wnt signaling pathway positive regulation of exosomal secretion positive regulation of extracellular exosome assembly cell adhesion cell-cell signaling response to organic substance

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CD138 Polyclonal Antibody

CD138 Antibody (36-2900) in IHC (P)

Product Details

36-2900

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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