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Invitrogen
CD14 Monoclonal Antibody (B-A8)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
CD14 Antibody (MA1-19240) in WB
Western blot was performed using CD14 Monoclonal Antibody (B-A8) (Product # MA1-19240) and a ~ 55 kDa band corresponding to CD14 was observed in mouse thymus which is reported to be positive and not in mouse heart. Tissue extracts (30 µg lysate) of mouse thymus (Lane 1) and mouse heart (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was pro... View More
Product Details
MA1-19240
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
B-A8
Immunogen
Human monocytes.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1071246
Product Specific Information
This antibody reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on the surface of monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.
Immunohistochemistry (Paraffin): Heat retrieval of antigen is recommended.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14
Gene Aliases: CD14
UniProt ID: (Human) P08571, (Mouse) P10810
Entrez Gene ID: (Human) 929, (Mouse) 12475
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
receptor-mediated endocytosis
phagocytosis
apoptotic process
inflammatory response
cell surface receptor signaling pathway
I-kappaB kinase/NF-kappaB signaling
response to heat
lipopolysaccharide-mediated signaling pathway
response to magnesium ion
positive regulation of type I interferon production
positive regulation of interferon-gamma production
positive regulation of tumor necrosis factor production
negative regulation of MyD88-independent toll-like receptor signaling pathway
toll-like receptor 4 signaling pathway
response to tumor necrosis factor
TRIF-dependent toll-like receptor signaling pathway
toll-like receptor TLR1:TLR2 signaling pathway
toll-like receptor TLR6:TLR2 signaling pathway
innate immune response
response to ethanol
positive regulation of endocytosis
positive regulation of cytokine secretion
response to electrical stimulus
necroptotic process
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to diacyl bacterial lipopeptide
cellular response to triacyl bacterial lipopeptide
apoptotic signaling pathway
response to molecule of bacterial origin
immune system process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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