Invitrogen

CD14 Monoclonal Antibody (Sa2-8), PerCP-Cyanine5.5, eBioscience™

28 Published Figures

25 References

View all (206) CD14 antibodies

Datasheet

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Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (28)

FIGURE: 1 / 29

CD14 Antibody (45-0141-82) in Flow

Staining of mouse thioglycolate-elicited peritoneal exudate cells with 0.25 µg of Rat IgG2a K Isotype Control PerCP-Cyanine5-5 (Product # 45-4321-80) (blue histogram) or 0.25 µg of Anti-Mouse CD14 PerCP-Cyanine5-5 (purple histogram). Total viable cells were used for analysis.

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Product Details

45-0141-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 25 publications 25 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Sa2-8

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_925733

Product Specific Information

Description: The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is a receptor for the complexes of LPS and LBP (LPS-Binding Protein) and is shown to associate with Toll-Like Receptor 4 (TLR4) and participate in the signaling and cellular response to bacterial LPS. In mouse, CD14 is expressed on the surface of macrophages and under certain conditions is also found in the serum in a secreted form. Sa2-8 has weak antagonistic activity (in NF-kappaB activation or TNF alpha production with LPS stimulation).

Applications Reported: This Sa2-8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Sa2-8 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis.

Neural regeneration research

Liu C,Guo S,Liu R,Guo M,Wang Q,Chai Z,Xiao B,Ma C

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2024

Umbilical cord mesenchymal stem cell-derived apoptotic extracellular vesicles ameliorate cutaneous wound healing in type 2 diabetic mice via macrophage pyroptosis inhibition.

Stem cell research & therapy

Wang Y,Jing L,Lei X,Ma Z,Li B,Shi Y,Zhang W,Li Y,Zhou H,Hu K,Xue Y,Jin Y

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2023

Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA.

PloS one

Liu T,Huang T,Li J,Li A,Li C,Huang X,Li D,Wang S,Liang M

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Wed Jul 19 00:00:00 EDT 2023

Macrophage polarization is involved in liver fibrosis induced by β ₁-adrenoceptor autoantibody.

Acta biochimica et biophysica Sinica

Wu Y,Fan X,Yu H,Liu J,Duan Y,Zhang S,Yan L,Du Y,Liu H

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2022

Early activation of the cardiac CX3CL1/CX3CR1 axis delays β-adrenergic-induced heart failure.

Scientific reports

Flamant M,Mougenot N,Balse E,Le Fèvre L,Atassi F,Gautier EL,Le Goff W,Keck M,Nadaud S,Combadière C,Boissonnas A,Pavoine C

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Thu Sep 09 00:00:00 EDT 2021

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Cell

Lee JY,Hall JA,Kroehling L,Wu L,Najar T,Nguyen HH,Lin WY,Yeung ST,Silva HM,Li D,Hine A,Loke P,Hudesman D,Martin JC,Kenigsberg E,Merad M,Khanna KM,Littman DR

45-0141 was used in Flow cytometry/Cell sorting to suggest that serum amyloid A proteins direct a pathogenic pro-inflammatory Th17 cell differentiation program and promote Th17-mediated inflammatory diseases.

Thu Jan 09 00:00:00 EST 2020

CD14⁺CD16^- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK.

Arthritis research & therapy

Xue J,Xu L,Zhu H,Bai M,Li X,Zhao Z,Zhong H,Cheng G,Li X,Hu F,Su Y

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Mon Sep 21 00:00:00 EDT 2020

CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.

Frontiers in cellular and infection microbiology

Shen TJ,Chen CL,Jhan MK,Tseng PC,Lin CF

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Thu Jun 17 00:00:00 EDT 2021

Leucine-rich repeats and calponin homology containing 4 (Lrch4) regulates the innate immune response.

The Journal of biological chemistry

Aloor JJ,Azzam KM,Guardiola JJ,Gowdy KM,Madenspacher JH,Gabor KA,Mueller GA,Lin WC,Lowe JM,Gruzdev A,Henderson MW,Draper DW,Merrick BA,Fessler MB

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Fri Feb 08 00:00:00 EST 2019

Chanzyme TRPM7 Mediates the Ca²⁺ Influx Essential for Lipopolysaccharide-Induced Toll-Like Receptor 4 Endocytosis and Macrophage Activation.

Immunity

Schappe MS,Szteyn K,Stremska ME,Mendu SK,Downs TK,Seegren PV,Mahoney MA,Dixit S,Krupa JK,Stipes EJ,Rogers JS,Adamson SE,Leitinger N,Desai BN

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Tue Jan 16 00:00:00 EST 2018

TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.

International journal of molecular medicine

Wu H,Xu X,Li J,Gong J,Li M

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

MicroRNA-10a/b are regulators of myeloid differentiation and acute myeloid leukemia.

Oncology letters

Bi L,Sun L,Jin Z,Zhang S,Shen Z

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro.

Oncology letters

Xiao T,Wu S,Yan C,Zhao C,Jin H,Yan N,Xu J,Wu Y,Li C,Shao Q,Xia S

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Age-Associated Microbial Dysbiosis Promotes Intestinal Permeability, Systemic Inflammation, and Macrophage Dysfunction.

Cell host & microbe

Thevaranjan N,Puchta A,Schulz C,Naidoo A,Szamosi JC,Verschoor CP,Loukov D,Schenck LP,Jury J,Foley KP,Schertzer JD,Larché MJ,Davidson DJ,Verdú EF,Surette MG,Bowdish DME

45-0141 was used in Flow cytometry/Cell sorting to study the potential of changes to the microbiota as a strategy for reducing age-associated inflammation and the accompanying morbidity.

Wed Apr 12 00:00:00 EDT 2017

Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling.

Nature communications

Pal B,Chen Y,Vaillant F,Jamieson P,Gordon L,Rios AC,Wilcox S,Fu N,Liu KH,Jackling FC,Davis MJ,Lindeman GJ,Smyth GK,Visvader JE

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Mon Nov 20 00:00:00 EST 2017

T3 Regulates a Human Macrophage-Derived TSH-β Splice Variant: Implications for Human Bone Biology.

Endocrinology

Baliram R,Latif R,Morshed SA,Zaidi M,Davies TF

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Antigen presenting cell abnormalities in the Cln3(-/-) mouse model of juvenile neuronal ceroid lipofuscinosis.

Biochimica et biophysica acta

Hersrud SL,Kovács AD,Pearce DA

45-0141 was used in Flow cytometry/Cell sorting to show that CLN3 deficiency alters antigen presenting cells, which can contribute to the autoimmune response in juvenile neuronal ceroid lipofuscinosis.

Fri Jul 01 00:00:00 EDT 2016

Mechanisms of Toll-like Receptor 4 Endocytosis Reveal a Common Immune-Evasion Strategy Used by Pathogenic and Commensal Bacteria.

Immunity

Tan Y,Zanoni I,Cullen TW,Goodman AL,Kagan JC

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2015

Role of caveolin-3 in lymphocyte activation.

Life sciences

Tran C,Stary CM,Schilling JM,Bentley B,Patel HH,Roth DM

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Thu Jan 15 00:00:00 EST 2015

CX₃CR1⁺ mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22.

The Journal of experimental medicine

Longman RS,Diehl GE,Victorio DA,Huh JR,Galan C,Miraldi ER,Swaminath A,Bonneau R,Scherl EJ,Littman DR

45-0141 was used in Flow cytometry/Cell sorting to study the role of CX3CR1+ mononuclear phagocytes in the development of colitis and disease progression following Citrobacter rodentium infection.

Mon Jul 28 00:00:00 EDT 2014

Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages.

The Journal of experimental medicine

Satpathy AT,KC W,Albring JC,Edelson BT,Kretzer NM,Bhattacharya D,Murphy TL,Murphy KM

45-0141 was used in Flow cytometry/Cell sorting to identify Zbtb46 as a transcription factor selectively expressed by classical DCs and their committed progenitors.

Mon Jun 04 00:00:00 EDT 2012

High-dose mannose-binding lectin therapy for Ebola virus infection.

The Journal of infectious diseases

Michelow IC,Lear C,Scully C,Prugar LI,Longley CB,Yantosca LM,Ji X,Karpel M,Brudner M,Takahashi K,Spear GT,Ezekowitz RA,Schmidt EV,Olinger GG

45-0141 was used in Flow cytometry/Cell sorting to investigate the effect of high-doses of mannose-binding lectin on Ebola virus infection.

Sat Jan 15 00:00:00 EST 2011

ICAM-5 modulates cytokine/chemokine production in the CNS during the course of herpes simplex virus type 1 infection.

Journal of neuroimmunology

Tse MC,Lane C,Mott K,Onlamoon N,Hsiao HM,Perng GC

45-0141 was used in Flow cytometry/Cell sorting to suggest that ICAM-5 plays a critical role in modulating chemokine production in the CNS.

Tue Aug 18 00:00:00 EDT 2009

Circadian mechanisms in murine and human bone marrow mesenchymal stem cells following dexamethasone exposure.

Bone

Wu X,Yu G,Parks H,Hebert T,Goh BC,Dietrich MA,Pelled G,Izadpanah R,Gazit D,Bunnell BA,Gimble JM

Published figure using CD14 monoclonal antibody (Product # 45-0141-82) in Flow Cytometry

Thu May 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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Gene Aliases: Cd14

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UniProt ID: (Mouse) P10810

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Entrez Gene ID: (Mouse) 12475

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Function(s)

lipopolysaccharide binding lipoteichoic acid binding transmembrane signal receptor

Process(es)

response to molecule of bacterial origin immune system process inflammatory response positive regulation of tumor necrosis factor production response to tumor necrosis factor innate immune response positive regulation of endocytosis positive regulation of cytokine secretion cellular response to lipopolysaccharide cellular response to lipoteichoic acid

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD14 Monoclonal Antibody (Sa2-8), PerCP-Cyanine5.5, eBioscience™

CD14 Antibody (45-0141-82) in Flow

Product Details

45-0141-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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