Invitrogen

CD144 (VE-cadherin) Monoclonal Antibody (16B1), PE, eBioscience™

19 Published Figures

23 References

View all (56) VE-cadherin (CD144) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (19)

CD144 (VE-cadherin) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 20

CD144 (VE-cadherin) Antibody (12-1449-82) in Flow

Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.5 µg of Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) or 0.5 µg of Anti-Human CD144 (VE-Cadherin) PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-1449-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

16B1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_763438

Product Specific Information

Description: The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

Applications Reported: This 16B1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 16B1 antibody has been tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Upregulation of ETV2 Expression Promotes Endothelial Differentiation of Human Dental Pulp Stem Cells.

Cell transplantation

Li J,Zhu Y,Li N,Wu T,Zheng X,Heng BC,Zou D,Xu J

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

Endothelial-derived cardiovascular disease-related microRNAs elevated with prolonged sitting pattern among postmenopausal women.

Scientific reports

Chang YJ,Tuz-Zahra F,Godbole S,Avitia Y,Bellettiere J,Rock CL,Jankowska MM,Allison MA,Dunstan DW,Rana B,Natarajan L,Sears DD

12-1449-82 was used in Flow Cytometry to explore the association between sitting patterns and microRNAs isolated from endothelial cell (EC)-derived EVs.

Thu Jun 03 00:00:00 EDT 2021

Local Mitochondrial ATP Production Regulates Endothelial Fatty Acid Uptake and Transport.

Cell metabolism

Ibrahim A,Yucel N,Kim B,Arany Z

12-1449-82 was used in Flow Cytometry to elucidate the mechanisms behind endothelial fatty acid uptake and transport.

Tue Aug 04 00:00:00 EDT 2020

Targeting QKI-7 in vivo restores endothelial cell function in diabetes.

Nature communications

Yang C,Eleftheriadou M,Kelaini S,Morrison T,González MV,Caines R,Edwards N,Yacoub A,Edgar K,Moez A,Ivetic A,Zampetaki A,Zeng L,Wilkinson FL,Lois N,Stitt AW,Grieve DJ,Margariti A

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2020

Vascular Tumor Recapitulated in Endothelial Cells from hiPSCs Engineered to Express the SERPINE1-FOSB Translocation.

Cell reports. Medicine

van IJzendoorn DGP,Salvatori DCF,Cao X,van den Hil F,Briaire-de Bruijn IH,de Jong D,Mei H,Mummery CL,Szuhai K,Bovée JVMG,Orlova VV

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Tue Dec 22 00:00:00 EST 2020

Angiogenic and Restorative Abilities of Human Mesenchymal Stem Cells Were Reduced Following Treatment With Serum From Diabetes Mellitus Type 2 Patients.

Journal of cellular biochemistry

Rezaie J,Mehranjani MS,Rahbarghazi R,Shariatzadeh MA

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Enhanced Therapeutic and Long-Term Dynamic Vascularization Effects of Human Pluripotent Stem Cell-Derived Endothelial Cells Encapsulated in a Nanomatrix Gel.

Circulation

Lee SJ,Sohn YD,Andukuri A,Kim S,Byun J,Han JW,Park IH,Jun HW,Yoon YS

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Tue Nov 14 00:00:00 EST 2017

MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells.

Stem cells translational medicine

Almalki SG,Llamas Valle Y,Agrawal DK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

A high-yield isolation and enrichment strategy for human lung microvascular endothelial cells.

Pulmonary circulation

Gaskill C,Majka SM

12-1449 was used in Flow cytometry/Cell sorting to describe a protocol to select large quantities of enriched primary human lung microvascular endothelial cells, free from mesenchymal contamination.

Wed Mar 01 00:00:00 EST 2017

ERK signaling is required for VEGF-A/VEGFR2-induced differentiation of porcine adipose-derived mesenchymal stem cells into endothelial cells.

Stem cell research & therapy

Almalki SG,Agrawal DK

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Fri May 12 00:00:00 EDT 2017

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

12-1449 was used in Flow cytometry/Cell sorting to identify HEB as a critical regulator of human mesodermal and hematopoietic specification.

Tue Sep 12 00:00:00 EDT 2017

Rab11a Mediates Vascular Endothelial-Cadherin Recycling and Controls Endothelial Barrier Function.

Arteriosclerosis, thrombosis, and vascular biology

Yan Z,Wang ZG,Segev N,Hu S,Minshall RD,Dull RO,Zhang M,Malik AB,Hu G

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Pten loss promotes MAPK pathway dependency in HER2/neu breast carcinomas.

Proceedings of the National Academy of Sciences of the United States of America

Ebbesen SH,Scaltriti M,Bialucha CU,Morse N,Kastenhuber ER,Wen HY,Dow LE,Baselga J,Lowe SW

12-1449-82 was used in Immunohistochemistry (Paraffin) to suggest that the MAPK pathway contributing to the maintenance of advanced breast cancers harboring Pten loss.

Tue Mar 15 00:00:00 EDT 2016

Endothelium and NOTCH specify and amplify aorta-gonad-mesonephros-derived hematopoietic stem cells.

The Journal of clinical investigation

Hadland BK,Varnum-Finney B,Poulos MG,Moon RT,Butler JM,Rafii S,Bernstein ID

12-1449 was used in Flow cytometry/Cell sorting to investigate the critical niche components and resident signals required for hematopoietic stem cell induction and self-renewal ex vivo.

Fri May 01 00:00:00 EDT 2015

Direct conversion of human amniotic cells into endothelial cells without transitioning through a pluripotent state.

Nature protocols

Ginsberg M,Schachterle W,Shido K,Rafii S

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo.

Biomaterials

Mendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2015

Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo.

Biomaterials

Mendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2015

Histone Demethylases KDM4A and KDM4C Regulate Differentiation of Embryonic Stem Cells to Endothelial Cells.

Stem cell reports

Wu L,Wary KK,Revskoy S,Gao X,Tsang K,Komarova YA,Rehman J,Malik AB

12-1449 was used in Flow cytometry/Cell sorting to demonstrate the essential role of KDM4A and KDM4C in murine embryonic stem cell differentiation to endothelial cells.

Tue Jul 14 00:00:00 EDT 2015

The isolation and culture of endothelial colony-forming cells from human and rat lungs.

Nature protocols

Alphonse RS,Vadivel A,Zhong S,McConaghy S,Ohls R,Yoder MC,Thébaud B

12-1449-82 was used in Immunohistochemistry to allow assessment of the functional state of these cells in experimental and human lung diseases, providing newer insights into their pathophysiological mechanisms.

Sun Nov 01 00:00:00 EDT 2015

Generation of Brain Microvascular Endothelial-Like Cells from Human Induced Pluripotent Stem Cells by Co-Culture with C6 Glioma Cells.

PloS one

Minami H,Tashiro K,Okada A,Hirata N,Yamaguchi T,Takayama K,Mizuguchi H,Kawabata K

12-1449 was used in Flow cytometry/Cell sorting to develop a protocol for generating human brain microvascular endothelial (BMEC)-like cells from induced pluripotent stem cells.

Tue Mar 15 00:00:00 EDT 2016

Differentiation of human pluripotent stem cells to cells similar to cord-blood endothelial colony-forming cells.

Nature biotechnology

Prasain N,Lee MR,Vemula S,Meador JL,Yoshimoto M,Ferkowicz MJ,Fett A,Gupta M,Rapp BM,Saadatzadeh MR,Ginsberg M,Elemento O,Lee Y,Voytik-Harbin SL,Chung HM,Hong KS,Reid E,O'Neill CL,Medina RJ,Stitt AW,Murphy MP,Rafii S,Broxmeyer HE,Yoder MC

12-1449 was used in Flow cytometry/Cell sorting to develop a protocol for converting human induced pluripotent or embryonic stem cells into endothelial colony-forming cells.

Sat Nov 01 00:00:00 EDT 2014

Identification of the hemogenic endothelial progenitor and its direct precursor in human pluripotent stem cell differentiation cultures.

Cell reports

Choi KD,Vodyanik MA,Togarrati PP,Suknuntha K,Kumar A,Samarjeet F,Probasco MD,Tian S,Stewart R,Thomson JA,Slukvin II

Published figure using CD144 (VE-cadherin) monoclonal antibody (Product # 12-1449-82) in Flow Cytometry

Thu Sep 27 00:00:00 EDT 2012

Novel surface targets and serum biomarkers from the ovarian cancer vasculature.

Cancer biology & therapy

Sasaroli D,Gimotty PA,Pathak HB,Hammond R,Kougioumtzidou E,Katsaros D,Buckanovich R,Devarajan K,Sandaltzopoulos R,Godwin AK,Scholler N,Coukos G

12-1449 was used in Flow cytometry/Cell sorting to identify novel tumor-specific vasculature markers which appear promising for cancer serum diagnostics, molecular imaging and/or therapeutic targeting applications and warrant further clinical development.

Mon Aug 01 00:00:00 EDT 2011

In vitro and in vivo evidence for lack of endovascular remodeling by third trimester trophoblasts.

Placenta

Kalkunte S,Lai Z,Tewari N,Chichester C,Romero R,Padbury J,Sharma S

12-1449 was used in Flow cytometry/Cell sorting to demonstrate that first and third trimester trophoblasts respond differentially to interactive signals from endothelial cells when cultured on matrigel.

Wed Oct 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; Vascular endothelial cadherin; VE-cadherin

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Gene Aliases: 7B4; CD144; CDH5

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UniProt ID: (Human) P33151

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Entrez Gene ID: (Human) 1003

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Function(s)

receptor binding calcium ion binding protein binding beta-catenin binding protein phosphatase binding ion channel binding cadherin

Process(es)

blood vessel maturation cell-cell junction assembly homophilic cell adhesion via plasma membrane adhesion molecules transforming growth factor beta receptor signaling pathway negative regulation of cell proliferation single organismal cell-cell adhesion adherens junction organization positive regulation of establishment of endothelial barrier regulation of establishment of cell polarity

Performance Guarantee

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CD144 (VE-cadherin) Monoclonal Antibody (16B1), PE, eBioscience™

CD144 (VE-cadherin) Antibody (12-1449-82) in Flow

Product Details

12-1449-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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