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CD146 Antibody (MA5-52424) in Flow
Flow cytometry analysis of Mouse MCAM(CD146) expression on BABL/c splenocytes. Samples were incubated with CD146 monoclonal antibody (Product # MA5-52424) and PE conjugated anti-mouse CD49b. The dot plots were derived from events with the forward and side light-scatter characteristics of intact cells.
Product Details
MA5-52424
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
241
Immunogen
Recombinant Mouse CD146/MCAM Protein (Val 24-Val563)
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
100 µg/mL
Purification
Protein A
Storage buffer
PBS with 0.05% BSA
Contains
0.03% ProClin 300
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types. In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly. CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development. CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD146; Cell surface glycoprotein MUC18; Gicerin; l-gicerin protein; melanoma adhesion molecule; Melanoma cell adhesion molecule; Melanoma-associated antigen MUC18
Gene Aliases: 1-gicerin; AV025631; CD146; CD149; Mcam; Muc18; s-endo; s-gicerin
UniProt ID: (Mouse) Q8R2Y2
Entrez Gene ID: (Mouse) 84004
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