Invitrogen

CD146 Monoclonal Antibody (P1H12), FITC, eBioscience™

FIGURE: 1 / 13

CD146 Antibody (11-1469-42) in Flow

Left: Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-CD146 FITC (purple histogram). Right: Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Anti-CD146 FITC.

Product Image Gallery

Product Details

11-1469-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rabbit

Published species

Dog, Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

P1H12

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2043805

Product Specific Information

Description: The monoclonal antibody P1H12 recognizes CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates.

The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat.

Applications Reported: This P1H12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and Human Umbilical Vein Cells (HUVEC). This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types. In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly. CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development. CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation.

Materials today. Bio

Pei YA,Mikaeiliagah E,Wang B,Zhang X,Pei M

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2023

The Stromal Vascular Fraction from Canine Adipose Tissue Contains Mesenchymal Stromal Cell Subpopulations That Show Time-Dependent Adhesion to Cell Culture Plastic Vessels.

Animals : an open access journal from MDPI

Scattini G,Pellegrini M,Severi G,Cagiola M,Pascucci L

11-1469-42 was used in Flow cytometry/Cell sorting to investigate their basic properties to speculate on the possible existence of mesenchymal stromal cells subpopulations.

Mon Mar 27 00:00:00 EDT 2023

Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension.

iScience

Al Hosni R,Bozec L,Roberts SJ,Cheema U

11-1469-42 was used in Flow cytometry/Cell sorting to uncover the significant contributions of the physical and physiological cell environment and highlights a chemically independent methodology for reprogramming and isolating skeletal progenitor cells from an adipose-derived cell population.

Fri Apr 15 00:00:00 EDT 2022

Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension.

iScience

Al Hosni R,Bozec L,Roberts SJ,Cheema U

Fri Apr 15 00:00:00 EDT 2022

The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject's Individual Age, Sex, Body Mass Index and Ethnicity.

Cells

Schmitz C,Alt C,Azares AR,Pearce DA,Facile TR,Furia JP,Maffulli N,Huang C,Alt EU

11-1469-42 was used in Flow cytometry/Cell sorting to show that key characteristics of unmodified, autologous, adipose-derived regenerative cells (UA-ADRCs) can be independent of the subject's age, sex, BMI and ethnicity.

Wed Dec 21 00:00:00 EST 2022

uPAR⁺ extracellular vesicles: a robust biomarker of resistance to checkpoint inhibitor immunotherapy in metastatic melanoma patients.

Journal for immunotherapy of cancer

Porcelli L,Guida M,De Summa S,Di Fonte R,De Risi I,Garofoli M,Caputo M,Negri A,Strippoli S,Serratì S,Azzariti A

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Site-Dependent Lineage Preference of Adipose Stem Cells.

Frontiers in cell and developmental biology

Wang T,Hill RC,Dzieciatkowska M,Zhu L,Infante AM,Hu G,Hansen KC,Pei M

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Wed Dec 14 00:00:00 EST 2022

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells.

Frontiers in bioengineering and biotechnology

Wang Y,Fu Y,Yan Z,Zhang XB,Pei M

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Wed Dec 14 00:00:00 EST 2022

LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

International journal of molecular medicine

Yu B,Li Q,Zhou M

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

International journal of molecular medicine

Yu B,Li Q,Zhou M

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro.

Stem cells international

Sun B,Luo X,Yang C,Liu P,Yang Y,Dong X,Yang Z,Xu J,Zhang Y,Li L

11-1469 was used in Flow cytometry/Cell sorting to demonstrate that urine-derived stem cells (USC) therapy significantly improves the renal function and histological damage, inhibited the inflammation and apoptosis processes in the kidney, and promoted tubular epithelial proliferation.

Thu May 13 00:00:00 EDT 2021

Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro.

Stem cells international

Sun B,Luo X,Yang C,Liu P,Yang Y,Dong X,Yang Z,Xu J,Zhang Y,Li L

Thu May 13 00:00:00 EDT 2021

Molecular Programming of Perivascular Stem Cell Precursors.

Stem cells (Dayton, Ohio)

Yianni V,Sharpe PT

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Targeting CD146 in combination with vorinostat for the treatment of ovarian cancer cells.

Oncology letters

Ma X,Wang J,Liu J,Mo Q,Yan X,Ma D,Duan H

11-1469 was used in Immunofluorescence to confirm that the induction of CD146 is a common phenomenon in vorinostat-treated ovarian cancer cells.

Wed Mar 01 00:00:00 EST 2017

A high-yield isolation and enrichment strategy for human lung microvascular endothelial cells.

Pulmonary circulation

Gaskill C,Majka SM

11-1469 was used in Flow cytometry/Cell sorting to describe a protocol to select large quantities of enriched primary human lung microvascular endothelial cells, free from mesenchymal contamination.

Wed Mar 01 00:00:00 EST 2017

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Nature communications

Somasundaram R,Zhang G,Fukunaga-Kalabis M,Perego M,Krepler C,Xu X,Wagner C,Hristova D,Zhang J,Tian T,Wei Z,Liu Q,Garg K,Griss J,Hards R,Maurer M,Hafner C,Mayerhöfer M,Karanikas G,Jalili A,Bauer-Pohl V,Weihsengruber F,Rappersberger K,Koller J,Lang R,Hudgens C,Chen G,Tetzlaff M,Wu L,Frederick DT,Scolyer RA,Long GV,Damle M,Ellingsworth C,Grinman L,Choi H,Gavin BJ,Dunagin M,Raj A,Scholler N,Gross L,Beqiri M,Bennett K,Watson I,Schaider H,Davies MA,Wargo J,Czerniecki BJ,Schuchter L,Herlyn D,Flaherty K,Herlyn M,Wagner SN

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2017

Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.

Stem cells (Dayton, Ohio)

Agarwal S,Loder SJ,Sorkin M,Li S,Shrestha S,Zhao B,Mishina Y,James AW,Levi B

Published figure using CD146 monoclonal antibody (Product # 11-1469-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Selected CD133⁺ progenitor cells to promote angiogenesis in patients with refractory angina: final results of the PROGENITOR randomized trial.

Circulation research

Jimenez-Quevedo P,Gonzalez-Ferrer JJ,Sabate M,Garcia-Moll X,Delgado-Bolton R,Llorente L,Bernardo E,Ortega-Pozzi A,Hernandez-Antolin R,Alfonso F,Gonzalo N,Escaned J,Bañuelos C,Regueiro A,Marin P,Fernandez-Ortiz A,Neves BD,Del Trigo M,Fernandez C,Tejerina T,Redondo S,Garcia E,Macaya C

11-1469 was used in Flow cytometry/Cell sorting to assess the safety and the feasibility of transendocardial injection of CD133(+) cells to foster angiogenesis in patients with refractory angina.

Fri Nov 07 00:00:00 EST 2014

Application of anodized titanium for enhanced recruitment of endothelial progenitor cells.

Nanoscale research letters

Moradi I,Behjati M,Kazemi M

11-1469 was used in Flow cytometry/Cell sorting to study the efficacy of an effective anodized titanium surface with enhanced attachment of endothelial progenitor cell.

Thu Jun 07 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD146; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Gicerin; l-gicerin protein; melanoma adhesion molecule; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen

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Gene Aliases: 1-gicerin; AV025631; CD146; CD149; MCAM; MUC18; s-endo; s-gicerin

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UniProt ID: (Human) P43121, (Mouse) Q8R2Y2

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Entrez Gene ID: (Rabbit) 100342848, (Human) 4162, (Dog) 489368, (Mouse) 84004

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Process(es)

angiogenesis glomerular filtration cell adhesion anatomical structure morphogenesis positive regulation of cell migration vascular wound healing heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules

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CD146 Monoclonal Antibody (P1H12), FITC, eBioscience™

CD146 Antibody (11-1469-42) in Flow

Product Details

11-1469-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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