Invitrogen

CD146 Monoclonal Antibody (P1H12), PE, eBioscience™

FIGURE: 1 / 12

CD146 Antibody (12-1469-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0037-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-CD146 PE (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

12-1469-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rabbit

Published species

Human, Mouse, Rabbit

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

P1H12

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11039529

Product Specific Information

Description: The monoclonal antibody P1H12 recognizes CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates.

The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat.

Applications Reported: This P1H12 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types. In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly. CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development. CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation.

Materials today. Bio

Pei YA,Mikaeiliagah E,Wang B,Zhang X,Pei M

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2023

Reprogramming bone progenitor identity and potency through control of collagen density and oxygen tension.

iScience

Al Hosni R,Bozec L,Roberts SJ,Cheema U

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

LGR5 expressing skin fibroblasts define a major cellular hub perturbed in scleroderma.

Cell

Gur C,Wang SY,Sheban F,Zada M,Li B,Kharouf F,Peleg H,Aamar S,Yalin A,Kirschenbaum D,Braun-Moscovici Y,Jaitin DA,Meir-Salame T,Hagai E,Kragesteen BK,Avni B,Grisariu S,Bornstein C,Shlomi-Loubaton S,David E,Shreberk-Hassidim R,Molho-Pessach V,Amar D,Tzur T,Kuint R,Gross M,Barboy O,Moshe A,Fellus-Alyagor L,Hirsch D,Addadi Y,Erenfeld S,Biton M,Tzemach T,Elazary A,Naparstek Y,Tzemach R,Weiner A,Giladi A,Balbir-Gurman A,Amit I

12-1469-42 was used in Flow cytometry/Cell sorting to find immune compartment dysfunction only in a specific subtype of diffuse Systemic sclerosis (scleroderma, SSc) patients but global dysregulation of the stromal compartment, particularly in a previously undefined subset of LGR5+-scleroderma-associated fibroblasts (ScAFs).

Thu Apr 14 00:00:00 EDT 2022

Matrix reverses immortalization-mediated stem cell fate determination.

Biomaterials

Wang Y,Hu G,Hill RC,Dzieciatkowska M,Hansen KC,Zhang XB,Yan Z,Pei M

12-1469-42 was used in Flow Cytometry to provide a promising model for in-depth investigation of the matrix protein influence on surrounding stem cell differentiation.

Fri Jan 01 00:00:00 EST 2021

uPAR⁺ extracellular vesicles: a robust biomarker of resistance to checkpoint inhibitor immunotherapy in metastatic melanoma patients.

Journal for immunotherapy of cancer

Porcelli L,Guida M,De Summa S,Di Fonte R,De Risi I,Garofoli M,Caputo M,Negri A,Strippoli S,Serratì S,Azzariti A

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

uPAR⁺ extracellular vesicles: a robust biomarker of resistance to checkpoint inhibitor immunotherapy in metastatic melanoma patients.

Journal for immunotherapy of cancer

Porcelli L,Guida M,De Summa S,Di Fonte R,De Risi I,Garofoli M,Caputo M,Negri A,Strippoli S,Serratì S,Azzariti A

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment.

eLife

Shan Z,Li L,Atkins CL,Wang M,Wen Y,Jeong J,Moreno NF,Feng D,Gui X,Zhang N,Lee CG,Elias JA,Lee WM,Gao B,Lam FW,An Z,Ju C

12-1469-42 was used in Flow Cytometry to unveil a critical role of Chi3l1 in hepatic platelet recruitment during AILI.

Thu Jun 10 00:00:00 EDT 2021

Site-Dependent Lineage Preference of Adipose Stem Cells.

Frontiers in cell and developmental biology

Wang T,Hill RC,Dzieciatkowska M,Zhu L,Infante AM,Hu G,Hansen KC,Pei M

12-1469 was used in Flow cytometry/Cell sorting to investigate the variance of depot-specific adult stem cells from adipose tissues.

Wed Dec 14 00:00:00 EST 2022

Site-Dependent Lineage Preference of Adipose Stem Cells.

Frontiers in cell and developmental biology

Wang T,Hill RC,Dzieciatkowska M,Zhu L,Infante AM,Hu G,Hansen KC,Pei M

12-1469 was used in Flow cytometry/Cell sorting to investigate the variance of depot-specific adult stem cells from adipose tissues.

Wed Dec 14 00:00:00 EST 2022

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

12-1469 was used in Flow cytometry/Cell sorting to demonstrate that transplanted MSCs promoted cutaneous wound healing partially through releasing apoptotic bodies which could convert the macrophages towards an anti-inflammatory phenotype that plays a crucial role in the tissue repair process.

Fri Nov 27 00:00:00 EST 2020

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Fri Nov 27 00:00:00 EST 2020

Intrinsic transcriptomic sex differences in human endothelial cells at birth and in adults are associated with coronary artery disease targets.

Scientific reports

Hartman RJG,Kapteijn DMC,Haitjema S,Bekker MN,Mokry M,Pasterkamp G,Civelek M,den Ruijter HM

12-1469 was used in Flow cytometry/Cell sorting to identify the transcriptomic sex differences in endothelial cells at birth and in adults.

Thu Jul 23 00:00:00 EDT 2020

Impact of Fibronectin Knockout on Proliferation and Differentiation of Human Infrapatellar Fat Pad-Derived Stem Cells.

Frontiers in bioengineering and biotechnology

Wang Y,Fu Y,Yan Z,Zhang XB,Pei M

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Wed Dec 14 00:00:00 EST 2022

LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

International journal of molecular medicine

Yu B,Li Q,Zhou M

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Combination of immunogenic oncolytic adenovirus ONCOS-102 with anti-PD-1 pembrolizumab exhibits synergistic antitumor effect in humanized A2058 melanoma huNOG mouse model.

Oncoimmunology

Kuryk L,Møller AW,Jaderberg M

12-1469 was used in Flow cytometry/Cell sorting to investigate the cytotoxicity of ONCOS-102 and pembrolizumab, an anti-PD-1 antibody, in four human melanoma cell lines, A375, A2058, SK-Mel-2 and SK-Mel-28.

Tue Jan 12 00:00:00 EST 2021

Molecular Programming of Perivascular Stem Cell Precursors.

Stem cells (Dayton, Ohio)

Yianni V,Sharpe PT

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets.

Journal of thrombosis and haemostasis : JTH

Yu Y,Gool E,Berckmans RJ,Coumans FAW,Barendrecht AD,Maas C,van der Wel NN,Altevogt P,Sturk A,Nieuwland R

12-1469 was used in Flow cytometry/Cell sorting to demonstrate that extracellular vesicles from human saliva facilitate homeostasis at sites of skin injury through exposure of coagulants TF and CD24.

Fri Jun 01 00:00:00 EDT 2018

Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.

Theranostics

Liu W,Zhang L,Xuan K,Hu C,Li L,Zhang Y,Jin F,Jin Y

12-1469 was used in Flow cytometry/Cell sorting to elucidate a novel role of tissue-nonspecific alkaline phosphatase in controlling bone marrow mesenchymal stem cell lineage selection.

Mon Sep 09 00:00:00 EDT 2019

Tumor-associated B-cells induce tumor heterogeneity and therapy resistance.

Nature communications

Somasundaram R,Zhang G,Fukunaga-Kalabis M,Perego M,Krepler C,Xu X,Wagner C,Hristova D,Zhang J,Tian T,Wei Z,Liu Q,Garg K,Griss J,Hards R,Maurer M,Hafner C,Mayerhöfer M,Karanikas G,Jalili A,Bauer-Pohl V,Weihsengruber F,Rappersberger K,Koller J,Lang R,Hudgens C,Chen G,Tetzlaff M,Wu L,Frederick DT,Scolyer RA,Long GV,Damle M,Ellingsworth C,Grinman L,Choi H,Gavin BJ,Dunagin M,Raj A,Scholler N,Gross L,Beqiri M,Bennett K,Watson I,Schaider H,Davies MA,Wargo J,Czerniecki BJ,Schuchter L,Herlyn D,Flaherty K,Herlyn M,Wagner SN

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2017

CD44-mediated monocyte transmigration across Cryptococcus neoformans-infected brain microvascular endothelial cells is enhanced by HIV-1 gp41-I90 ectodomain.

Journal of biomedical science

He X,Shi X,Puthiyakunnon S,Zhang L,Zeng Q,Li Y,Boddu S,Qiu J,Lai Z,Ma C,Xie Y,Long M,Du L,Huang SH,Cao H

12-1469 was used in Immunopurification to examine the future prospects to inflammatory responses induced by HIV-1 gp41-I90 ectodomain and to effectively eliminate the opportunistic infections in AIDS patients.

Sat Feb 20 00:00:00 EST 2016

Analysis of Bone-Cartilage-Stromal Progenitor Populations in Trauma Induced and Genetic Models of Heterotopic Ossification.

Stem cells (Dayton, Ohio)

Agarwal S,Loder SJ,Sorkin M,Li S,Shrestha S,Zhao B,Mishina Y,James AW,Levi B

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer.

Cell cycle (Georgetown, Tex.)

Qin Y,Qin J,Zhou C,Li J,Gao WQ

12-1469 was used in Flow cytometry/Cell sorting to demonstrates that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning.

Tue Jan 26 00:00:00 EST 2016

Kupffer cells potentiate liver sinusoidal endothelial cell injury in sepsis by ligating programmed cell death ligand-1.

Journal of leukocyte biology

Hutchins NA,Wang F,Wang Y,Chung CS,Ayala A

12-1469 was used in Flow cytometry/Cell sorting to study Kupffer cell-mediated potentiation of liver sinusoidal endothelial cell injury in sepsis, by ligating programmed cell death ligand-1.

Fri Nov 01 00:00:00 EDT 2013

Application of anodized titanium for enhanced recruitment of endothelial progenitor cells.

Nanoscale research letters

Moradi I,Behjati M,Kazemi M

Published figure using CD146 monoclonal antibody (Product # 12-1469-42) in Flow Cytometry

Thu Jun 07 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD146; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Gicerin; l-gicerin protein; melanoma adhesion molecule; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen

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Gene Aliases: 1-gicerin; AV025631; CD146; CD149; MCAM; MUC18; s-endo; s-gicerin

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UniProt ID: (Human) P43121, (Mouse) Q8R2Y2

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Entrez Gene ID: (Rabbit) 100342848, (Human) 4162, (Dog) 489368, (Mouse) 84004

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Process(es)

angiogenesis glomerular filtration cell adhesion anatomical structure morphogenesis positive regulation of cell migration vascular wound healing heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules

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CD146 Monoclonal Antibody (P1H12), PE, eBioscience™

CD146 Antibody (12-1469-42) in Flow

Product Details

12-1469-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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