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Product Details
66153-1-IG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4D8A9
Immunogen
CD146/MCAM Fusion Protein Ag11855 (26-350 aa encoded by BC056418)
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.
Immunogen sequence: GEAEQPAPE LVEVEVGSTA LLKCGLSQSQ GNLSHVDWFS VHKEKRTLIF RVRQGQGQSE PGEYEQRLSL QDRGATLALT QVTPQDERIF LCQGKRPRSQ EYRIQLRVYK APEEPNIQVN PLGIPVNSKE PEEVATCVGR NGYPIPQVIW YKNGRPLKEE KNRVHIQSSQ TVESSGLYTL QSILKAQLVK EDKDAQFYCE LNYRLPSGNH MKESREVTVP VFYPTEKVWL EVEPVGMLKE GDRVEIRCLA DGNPPPHFSI SKQNPSTREA EEETTNDNGV LVLEPARKEH SGRYECQGLD LDTMISLLSE PQELLVNYVS DVRVSP (26-350 aa encoded by BC056418)
Target Information
CD146 (MCAM) is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 is expressed at cell-cell junctions in all endothelial cells, and has also been observed in other normal and malignant cell types. In addition to its recognized role as a calcium-independent cell adhesion molecule, CD146 may also act as a signal transduction molecule in the recruitment of Fyn kinase, and in the subsequent tyrosine-phosphorylation of intracellular proteins involved in actin cytoskeleton assembly. CD146 plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. CD146 expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Further, CD146 could be an adhesion molecule active in neural crest cells during embryonic development. CD146 acts also as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD146; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Gicerin; melanoma adhesion molecule; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
Gene Aliases: CD146; MCAM; MUC18
UniProt ID: (Human) P43121
Entrez Gene ID: (Human) 4162
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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