Invitrogen

CD15 Monoclonal Antibody (MMA (LeuM1))

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CD15 Antibody (MA5-11789) in Flow

Flow cytometry analysis of FUT4 in blood granulocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-11789) at a dilution of 2 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLig... View More

CD15 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-11789

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

MMA (LeuM1)

Immunogen

U937 histiocytic cell line

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986179

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Clonogenic CD15 immunoreactive radial glial cells from the developing human lateral ganglionic eminence.

Current pharmaceutical biotechnology

Buttler D,Mai JK,Ashwell KW,Andressen C

MA5-11789 was used in immunocytochemistry to study the sub-population of CD15-positive radial glial cells in the developing human lateral ganglionic eminence

Fri Aug 09 00:00:00 EDT 2013

Folliculocentric B-cell-rich follicular dendritic cells sarcoma: a hitherto unreported morphological variant mimicking lymphoproliferative disorders.

Human pathology

Lorenzi L,Lonardi S,Petrilli G,Tanda F,Bella M,Laurino L,Rossi G,Facchetti F

MA5-11789 was used in immunohistochemistry to study three clinical cases of follicular dendritic cell sarcoma

Wed Feb 01 00:00:00 EST 2012

Citraconic anhydride: a new antigen retrieval solution.

Pathology

Leong AS,Haffajee Z

MA5-11789 was used in immunohistochemistry - paraffin section to discuss factors that influence heat-induced antigen retrieval

Fri Jan 01 00:00:00 EST 2010

Metastasis to the breast from an adenocarcinoma of the lung with extensive micropapillary component: a case report and review of the literature.

Diagnostic pathology

Maounis N,Chorti M,Legaki S,Ellina E,Emmanouilidou A,Demonakou M,Tsiafaki X

MA5-11789 was used in immunohistochemistry to report on a case of metastasis to the breast from an adenocarcinoma of the lung

Fri Dec 17 00:00:00 EST 2010

Secondary involvement of the breast in T-cell non-Hodgkin lymphoma, an unusual example mimicking inflammatory breast carcinoma.

Archives of gynecology and obstetrics

Kelten C,Kabukcu S,Sen N,Teke Z,Yaren A,Erdem E,Duzcan E

MA5-11789 was used in immunohistochemistry to report a clinical case of T-cell non-Hodgkin lymphoma with unusual inflammatory breast carcinoma similarity

Wed Jul 01 00:00:00 EDT 2009

Innate and adaptive immunity during epileptogenesis and spontaneous seizures: evidence from experimental models and human temporal lobe epilepsy.

Neurobiology of disease

Ravizza T,Gagliardi B,Noé F,Boer K,Aronica E,Vezzani A

MA5-11789 was used in immunohistochemistry to study the role of innate and adaptive immunity in epileptogenesis and spontaneous seizures

Tue Jan 01 00:00:00 EST 2008

Inflammatory processes in cortical tubers and subependymal giant cell tumors of tuberous sclerosis complex.

Epilepsy research

Boer K,Jansen F,Nellist M,Redeker S,van den Ouweland AM,Spliet WG,van Nieuwenhuizen O,Troost D,Crino PB,Aronica E

MA5-11789 was used in immunocytochemistry to study inflammatory processes in cerebral lesions associated with tuberous sclerosis complex

Tue Jan 01 00:00:00 EST 2008

Human cortical neurons originate from radial glia and neuron-restricted progenitors.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mo Z,Moore AR,Filipovic R,Ogawa Y,Kazuhiro I,Antic SD,Zecevic N

MA5-11789 was used in immunocytochemistry to investigate the derivation of cortical neurons from radial glia and neuron-restricted progenitors

Wed Apr 11 00:00:00 EDT 2007

Biphasic malignant mesothelioma cases with osseous differentiation and long survival: a review of the literature.

Lung cancer (Amsterdam, Netherlands)

Demirag F,Unsal E,Tastepe I

MA5-11789 was used in immunohistochemistry to report two clinical cases of biphasic malignant mesothelioma with osseous differentiation

Wed Aug 01 00:00:00 EDT 2007

Clear cell adenocarcinoma of the tunica vaginalis of the testis with an adjacent uterus-like tissue.

Pathology international

Tulunay O,Gögüş C,Baltaci S,Bulut S

MA5-11789 was used in immunohistochemistry to report on a case of paratesticular clear cell carcinoma of the tunica vaginalis of the testis

Sun Aug 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1

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Gene Aliases: CD15; ELFT; FCT3A; FUC-TIV; FUT4; FUTIV; LeX; SSEA-1

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UniProt ID: (Human) P22083

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Entrez Gene ID: (Human) 2526

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Function(s)

fucosyltransferase activity alpha-(1->3)-fucosyltransferase activity glycosyltransferase

Process(es)

carbohydrate metabolic process protein glycosylation protein N-linked glycosylation fucosylation L-fucose catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD15 Monoclonal Antibody (MMA (LeuM1))

CD15 Antibody (MA5-11789) in Flow

Product Details

MA5-11789

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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