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Invitrogen
CD150 Monoclonal Antibody (A12 (7D4)), FITC, eBioscience™
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
CD150 Antibody (11-1509-42) in Flow
Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 APC, CD19 PE-Cy7 and CD150 FITC. As expected based on known relative expression patterns, CD150 clone A12 (7D4) stains monocytes (orange), granulocytes (blue) and a subset of lymphocytes (pink).
Product Details
11-1509-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
A12 (7D4)
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10669579
Product Specific Information
Description: The A12 monoclonal antibody reacts with human CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM induces proliferation and cytokine production of T cells and SLAM plays a role in activation of T and B cells.
Applications Reported: This A12 (7D4) antibody has been reported for use in flow cytometric analysis.
Applications Tested: The A12 (7D4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg)/test per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells. Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD150; CD150 antigen; CDw150; IPO-3; Signaling lymphocytic activation molecule; SLAM family member 1
Gene Aliases: CD150; CDw150; SLAM; SLAMF1
UniProt ID: (Human) W0HKK7
Entrez Gene ID: (Human) 6504
natural killer cell differentiation
natural killer cell proliferation
myeloid dendritic cell activation involved in immune response
negative regulation of T cell cytokine production
phagocytosis
cell adhesion
signal transduction
positive regulation of cell proliferation
regulation of vesicle fusion
negative regulation of interleukin-12 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
T-helper 1 cell cytokine production
positive regulation of activated T cell proliferation
innate immune response
positive regulation of JNK cascade
viral entry into host cell
regulation of catalytic activity
positive regulation of ERK1 and ERK2 cascade
negative regulation of interferon-gamma secretion
positive regulation of interferon-gamma secretion
negative regulation of CD40 signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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