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Invitrogen
CD152 (CTLA-4) Monoclonal Antibody (WKH203), PE, eBioscience™
-Flow-20170216130641.jpg?time=20220826 "CD152 (CTLA-4) Antibody in Flow Cytometry (Flow)")
-Flow-20170216130641.jpg?time=20220826)
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD152 (CTLA-4) Antibody (12-1520-82) in Flow")
Product Details
12-1520-82
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
WKH203
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_465877
Product Specific Information
Description: The WKH203 monoclonal antibody reacts with rat CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling.
Applications Reported: The WKH203 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This WKH203 antibody has been tested by flow cytometric analysis of unstimulated and Con A-stimulated (3 days) rat splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD152 antigen; cytotoxic T-lymphocyte protein 4; ICOS; soluble form; transmembrane form
Gene Aliases: Cd152; CTLA-4; sCTLA4
Entrez Gene ID: (Rat) 63835
immune response
cellular response to DNA damage stimulus
negative regulation of B cell proliferation
negative regulation of T cell proliferation
positive regulation of apoptotic process
negative regulation of regulatory T cell differentiation
negative regulation of immune response
B cell receptor signaling pathway
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