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Invitrogen
CD153 Chimeric Recombinant Rabbit Monoclonal Antibody (RM153)
FIGURE: 1 / 1
CD153 Chimeric Antibody (MA5-48166) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of CD153 in RAW264.7 cells using CD153 Chimeric Monoclonal Antibody (Product # MA5-48166). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with CD153 Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from lef... View More
Product Details
MA5-48166
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM153
Immunogen
This antibody was raised by immunising Sprague Dawley rats with the CHO cells stably transfected with murine CD153 and fusing the splenocytes with P3U1 myeloma cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074609
Product Specific Information
Specificity: This antibody is specific for murine CD153, a 40-kDa type II transmembrane protein member of the TNF superfamily. Interaction of CD30 with CD153 plays an important role in T cell activation/costimulation.
Target Information
The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and the IL4 receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD153; CD30 ligand; CD30-L; CD30ligand; Tumor necrosis factor ligand superfamily member 8
Gene Aliases: CD153; Cd30l; CD30LG; Tnfsf8
UniProt ID: (Mouse) P32972
Entrez Gene ID: (Mouse) 21949
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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