Invitrogen

CD154 (CD40 Ligand) Monoclonal Antibody (24-31), PE, eBioscience™

CD154 (CD40 Ligand) Antibody in Flow Cytometry (Flow)

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CD154 (CD40 Ligand) Antibody (12-1548-42) in Flow

Human peripheral blood cells unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (500X) (Product # 00-4970-03) (right) were stained with Anti-Human CD8a FITC (Product # 11-0088-42) and Anti-Human CD154 (CD40 Ligand) PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-1548-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Cynomolgus monkey, Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

24-31

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1963568

Product Specific Information

Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.

Applications Reported: 24-31 has been reported for use in flow cytometric analysis.

Applications Tested: This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of activated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 12-1548-42) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

T Cell Responses Induced by Attenuated Flavivirus Vaccination Are Specific and Show Limited Cross-Reactivity with Other Flavivirus Species.

Journal of virology

Grifoni A,Voic H,Dhanda SK,Kidd CK,Brien JD,Buus S,Stryhn A,Durbin AP,Whitehead S,Diehl SA,De Silva AD,Balmaseda A,Harris E,Weiskopf D,Sette A

12-1548 was used in Flow cytometry/Cell sorting to test pools of epitopes derived from dengue (DENV), Zika (ZIKV), Japanese encephalitis (JEV), West Nile (WNV), and yellow fever (YFV) viruses by intracellular cytokine staining (ICS) using peripheral blood mononuclear cells (PBMCs) of individuals naturally exposed to DENV or immunized with DENV (TV005) or YF17D vaccine.

Mon May 04 00:00:00 EDT 2020

Application of Flow Cytometry in the Diagnostics Pipeline of Primary Immunodeficiencies Underlying Disseminated Talaromyces marneffei Infection in HIV-Negative Children.

Frontiers in immunology

Lee PP,Lao-Araya M,Yang J,Chan KW,Ma H,Pei LC,Kui L,Mao H,Yang W,Zhao X,Trakultivakorn M,Lau YL

12-1548-42 was used in Flow Cytometry to show that this simple and rapid diagnostic algorithm will be useful in guiding genetic studies for patients with disseminated talaromycosis requiring immunological investigations.

Mon Oct 26 00:00:00 EDT 2020

Photodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70.

Oncology letters

Zhang F,Zhu Y,Fan G,Hu S

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 12-1548-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Targeted gene editing restores regulated CD40L function in X-linked hyper-IgM syndrome.

Blood

Hubbard N,Hagin D,Sommer K,Song Y,Khan I,Clough C,Ochs HD,Rawlings DJ,Scharenberg AM,Torgerson TR

12-1548 was used in Flow cytometry/Cell sorting to demonstrate the feasibility of engineered nuclease-directed gene repair to restore endogenously regulated CD40L, and the potential for its use in T-cell therapy for X-linked hyper-IgM syndrome syndrome.

Thu May 26 00:00:00 EDT 2016

Endothelial microparticles interact with and support the proliferation of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wheway J,Latham SL,Combes V,Grau GE

12-1548 was used in Flow cytometry/Cell sorting to explore the effect of endothelial cell microparticles in T cell activation and antigen presentation.

Wed Oct 01 00:00:00 EDT 2014

Isolation of biologically-active exosomes from human plasma.

Journal of immunological methods

Muller L,Hong CS,Stolz DB,Watkins SC,Whiteside TL

12-1548 was used in Flow cytometry/Cell sorting to isolate and study the effects of exosomes present in human plasma on immune cells.

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD154; CD40 antigen ligand; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

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Gene Aliases: CD154; CD40L; CD40LG; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP

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UniProt ID: (Human) P29965

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Entrez Gene ID: (Human) 959, (Cynomolgus monkey) 102138630

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Function(s)

cytokine activity tumor necrosis factor receptor binding CD40 receptor binding protein binding

Process(es)

inflammatory response leukocyte cell-cell adhesion activation of JUN kinase activity platelet activation B cell differentiation T cell costimulation positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-4 production tumor necrosis factor-mediated signaling pathway B cell proliferation positive regulation of T cell proliferation negative regulation of apoptotic process isotype switching immunoglobulin secretion regulation of immune response regulation of immunoglobulin secretion positive regulation of NF-kappaB transcription factor activity positive regulation of endothelial cell apoptotic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD154 (CD40 Ligand) Monoclonal Antibody (24-31), PE, eBioscience™

CD154 (CD40 Ligand) Antibody (12-1548-42) in Flow

Product Details

12-1548-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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