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Invitrogen
CD154 (CD40 Ligand) Monoclonal Antibody (MR-1)
FIGURE: 1 / 2
CD154 (CD40 Ligand) Antibody (MA1-10108) in Flow
Separation of CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in flow cytometry analysis (surface staining) of PMA, ionomycin and LPS stimulated mouse splenocytes stained using anti-mouse CD154 (MR-1) purified Monoclonal antibody (Product # MA1-10108). Low endotoxin, concentration in sample 3 µg/mL, GAArH APC.
Product Details
MA1-10108
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MR-1
Immunogen
Activated mouse Th1 clone D1.6
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11153361
Product Specific Information
This antibody detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Functional Application: Blocking.
Target Information
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD154; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5
Gene Aliases: CD154; CD40-L; Cd40l; Cd40lg; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP
UniProt ID: (Mouse) P27548
Entrez Gene ID: (Mouse) 21947
positive regulation of immune response to tumor cell
inflammatory response
immune response
signal transduction
platelet activation
B cell differentiation
positive regulation of B cell proliferation
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
B cell proliferation
negative regulation of apoptotic process
isotype switching
positive regulation of MHC class II biosynthetic process
immunoglobulin secretion
regulation of immunoglobulin secretion
dendritic cell differentiation
positive regulation of endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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