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Invitrogen
CD162 (PSGL-1) Monoclonal Antibody (FLEG), Super Bright™ 436, eBioscience™
FIGURE: 1 / 1
CD162 (PSGL-1) Antibody (62-1629-42) in Flow
Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control Super Bright 436 (Product # 62-4724) (blue histogram) or Anti-Human CD162 (PSGL-1) Super Bright 436. Cells in the lymphocyte (pink histogram) or granulocyte (orange histogram) gate were used for analysis.
Product Details
62-1629-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
FLEG
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2663290
Product Specific Information
Description: This FLEG monoclonal antibody recognizes human CD162, which is also known as P-selectin glyocoprotein ligand-1 (PSGL-1). This 120-kDa protein exists as a homodimer on the surface of monocytes, neutrophils, granulocytes, peripheral T cells, some B cells, and a subset of CD34+ hematopoietic progenitor cells (HPCs) in the bone marrow. CD162 binds CD62P (P-selectin), CD62E (E-selectin), and CD62L (L-selectin) to mediate leukocyte interactions with each other as well as with activated platelets and endothelium. Thus, CD162 plays a critical role in leukocyte adhesion and rolling. Furthermore, the binding of CD162 to P-selectin on HPCs has been demonstrated to inhibit hematopoiesis. Studies have also demonstrated CD162 as the receptor for enterovirus 71.
Applications Reported: This FLEG antibody has been reported for use in flow cytometric analysis.
Applications Tested: This FLEG antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD162 (PSGL-1) is a 120 kDa homodimer protein found on the surface of leukocytes and platelets. CD162 is known to bind CD62P (P-selectin), CD62L (L-selectin), and to a lower degree CD62E (E-selectin). These interactions play a critical role in leukocyte adhesion, rolling and extravasation. CD162 has been shown to be an important immune marker of T cell exhaustion in chronic viral infections and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD162; Cutaneous lymphocyte associated antigen; cutaneous lymphocyte-associated associated antigen; P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; SELPG
Gene Aliases: CD162; CLA; PSGL-1; PSGL1; SELPLG
UniProt ID: (Human) Q14242
Entrez Gene ID: (Human) 6404
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