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Invitrogen
CD163 Monoclonal Antibody (MAC 2-158), Alexa Fluor™ 647, eBioscience™
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
CD163 Antibody (51-1637-42) in Flow
Normal human peripheral blood cells were stained with CD11b Monoclonal Antibody, PE-Cyanine7 (Product # 25-0118-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 647 (Product # 51-4714-81) (left) or CD163 Monoclonal Antibody, Alexa Fluor 647 (right). Cells in the monocyte gate were used for analysis.
Product Details
51-1637-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MAC 2-158
Immunogen
Human monocytes stimulated with IFN gamma and dexamethasone
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2811844
Product Specific Information
Description: This MAC 2-158 monoclonal antibody reacts with human CD163, a high affinity macrophage scavenger receptor for hemoglobin and haptoglobin.
Applications Reported: This MAC 2-158 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MAC 2-158 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
Target Information
CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD163; Hemoglobin scavenger receptor; macrophage-associated antigen; Scavenger receptor cysteine-rich type 1 protein M130; Soluble sCD163
Gene Aliases: CD163; M130; MM130; SCARI1
UniProt ID: (Human) Q86VB7
Entrez Gene ID: (Human) 9332
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