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FIGURE: 1 / 2
CD177 Antibody (PA5-93050) in WB
Western blot analysis of CD177 in extracts of various lysates. Samples were incubated with CD177 Polyclonal antibody (Product # PA5-93050) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-93050
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human CD177 (NP_0651392)
Conjugate
Form
Liquid
Concentration
0.74 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806603
Product Specific Information
Immunogen sequence: VSDLPRQWTP KNTSCDSGLG CQDTLMLIES GPQVSLVLSK GCTEAKDQEP RVTEHRMGPG LSLISYTFVC RQEDFCNNLV NSLPLWAPQP PADPGSLRCP VCLSMEGCLE GTTEEICPKG TTHCYDGLLR LRGGGIFSNL RVQGCMPQPV CNLLNGTQEI GPVGMTENCD MKDFLTCHRG TTIMTHGNLA QEPTDWTTSN TEMCEVGQVC QETLLLLDVG LTSTLVGTKG CSTVGAQNSQ KTTIHSAPPG V; Positive Samples: BT-474; Cellular Location: Cell membrane, GPI-anchor, Lipid-anchor
Target Information
CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD177; CD177 antigen; cell surface receptor; HNA-2a; Human neutrophil alloantigen 2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1
Gene Aliases: 1190003K14Rik; CD177; HNA-2a; HNA2A; NB1; NB1 GP; Pdp3; PRV-1; PRV1; UNQ595/PRO1181
UniProt ID: (Human) Q8N6Q3, (Mouse) Q8R2S8
Entrez Gene ID: (Human) 57126, (Mouse) 68891
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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