Invitrogen

CD178 (Fas Ligand) Monoclonal Antibody (NOK-1), PE, eBioscience™

CD178 (Fas Ligand) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD178 (Fas Ligand) Antibody (12-9919-42) in Flow

Surface staining of human FasL tranfected cells with Anti-Human CD178 (CD95 Ligand) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-9919-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NOK-1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834378

Product Specific Information

Description: The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended.

Applications Reported: NOK-1 has been reported for use in flow cytometric analysis.

Applications Tested: This NOK-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR. CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses. CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where CD178 is expressed predominantly on activated T cells. FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity. CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension. The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity.

Nature communications

Bernareggi D,Xie Q,Prager BC,Yun J,Cruz LS,Pham TV,Kim W,Lee X,Coffey M,Zalfa C,Azmoon P,Zhu H,Tamayo P,Rich JN,Kaufman DS

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 12-9919-42) in Flow Cytometry

Thu Apr 07 00:00:00 EDT 2022

Balanced engagement of activating and inhibitory receptors mitigates human NK cell exhaustion.

JCI insight

Myers JA,Schirm D,Bendzick L,Hopps R,Selleck C,Hinderlie P,Felices M,Miller JS

12-9919-42 was used in Flow cytometry/Cell sorting to uncover some of the mechanisms underlying NK cell exhaustion in humans and establish our novel in vitro model as a valuable tool for studying the processes regulating exhaustion.

Mon Aug 08 00:00:00 EDT 2022

Silencing of soluble epoxide hydrolase 2 gene reduces H₂O₂-induced oxidative damage in rat intestinal epithelial IEC-6 cells via activating PI3K/Akt/GSK3β signaling pathway.

Cytotechnology

Li J,Luo J,Zhang Y,Tang C,Wang J,Chen C

12-9919 was used in Western Blotting to explore the effects and molecular mechanism of siEPHX2 on H2O2-induced oxidative damage in rat intestinal epithelial IEC-6 cells.

Sat Feb 01 00:00:00 EST 2020

Interleukin-24 Regulates T Cell Activity in Patients With Colorectal Adenocarcinoma.

Frontiers in oncology

Zhang Y,Liu Y,Xu Y

12-9919-42 was used in Flow Cytometry to indicate that IL-24 might regulate T cell function in a dose-dependent manner.

Wed Sep 30 00:00:00 EDT 2020

Transmembrane TNF-α promotes activation-induced cell death by forward and reverse signaling.

Oncotarget

Zhang M,Wang J,Jia L,Huang J,He C,Hu F,Yuan L,Wang G,Yu M,Li Z

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 12-9919-42) in Flow Cytometry

Fri Sep 08 00:00:00 EDT 2017

IL12-mediated sensitizing of T-cell receptor-dependent and -independent tumor cell killing.

Oncoimmunology

Braun M,Ress ML,Yoo YE,Scholz CJ,Eyrich M,Schlegel PG,Wölfl M

12-9919 was used in Flow cytometry/Cell sorting to investigate how IL12, and other inflammatory cytokines, shape effector functions of human T-cells.

Fri Jul 01 00:00:00 EDT 2016

Long-term effect on natural killer cells by interferon-α therapy on the outcomes of HCV infection.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Hu X,Jiang Y,Li X,Gao Y,Guo X,Chi X,Yan H,Feng J,Zhong J,Sun B,Shao X,Xiao W,Pan Y,Niu J

12-9919 was used in Flow cytometry/Cell sorting to investigate the dynamic changes in frequency of different natural killer cell subsets following treatment in xx chronic hepatitis C patients.

Thu May 01 00:00:00 EDT 2014

Fas-mediated killing of primary prostate cancer cells is increased by mitoxantrone and docetaxel.

Molecular cancer therapeutics

Symes JC,Kurin M,Fleshner NE,Medin JA

12-9919 was used in Flow cytometry/Cell sorting to investigate the susceptibility of cultured primary prostate cells to Fas-mediated apoptosis.

Mon Sep 01 00:00:00 EDT 2008

c-FLIP inhibits chemotherapy-induced colorectal cancer cell death.

Oncogene

Longley DB,Wilson TR,McEwan M,Allen WL,McDermott U,Galligan L,Johnston PG

12-9919 was used in Flow cytometry/Cell sorting to study the effect of c-FLIP on the apoptotic response to chemotherapies used in colorectal cancer.

Thu Feb 09 00:00:00 EST 2006

Siva-1 negatively regulates NF-kappaB activity: effect on T-cell receptor-mediated activation-induced cell death (AICD).

Oncogene

Gudi R,Barkinge J,Hawkins S,Chu F,Manicassamy S,Sun Z,Duke-Cohan JS,Prasad KV

12-9919 was used in Western Blotting to demonstrate a significant role for endogenous Siva-1, through its inhibitory effect on NF-kappaB activity, in TCR-mediated activation-induced cell death (AICD).

Thu Jun 08 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Apoptosis (APO 1) antigen ligand 1; apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APTL; CD178; CD178 antigen; CD95 ligand; CD95 ligand protein;Generalized lymphoproliferative disease (Gld); CD95-L; Fas antigen ligand; Fas ligand; Fas ligand (TNF superfamily, member 6); Fas-LG; mutant tumor necrosis factor family member 6; soluble form; TNFL; Tumor necrosis factor (ligand) superfamily member 6; Tumor necrosis factor ligand; tumor necrosis factor ligand 1A; Tumor necrosis factor ligand superfamily member 6; Tumor necrosis factor ligand superfamily member 6 (TNFL6 / TNFSF6)

View more View less

Gene Aliases: ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; FASL; FASLG; TNFSF6; TNLG1A

View more View less

UniProt ID: (Human) P48023

View more View less

Entrez Gene ID: (Human) 356

View more View less

Function(s)

receptor binding death receptor binding cytokine activity tumor necrosis factor receptor binding protein binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory cell apoptotic process immune response signal transduction cell-cell signaling positive regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors negative regulation of angiogenesis cellular chloride ion homeostasis response to lipopolysaccharide positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of neuron apoptotic process positive regulation of epidermal growth factor receptor signaling pathway retinal cell programmed cell death endosomal lumen acidification T cell apoptotic process necroptotic process response to growth factor apoptotic signaling pathway extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway necroptotic signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of endothelial cell apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD178 (Fas Ligand) Monoclonal Antibody (NOK-1), PE, eBioscience™

CD178 (Fas Ligand) Antibody (12-9919-42) in Flow

Product Details

12-9919-42

Western Blot (WB)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!