Proteintech

CD18 Polyclonal Antibody

CD18 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD18 Antibody (10554-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 10554-1-AP ( CD18 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CD18 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

10554-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CD18 Fusion Protein Ag0861 (469-769 aa encoded by BC005861)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ICRCDTGYI GKNCECQTQG RSSQELEGSC RKDNNSIICS GLGDCVCGQC LCHTSDVPGK LIYGQYCECD TINCERYNGQ VCGGPGRGLC FCGKCRCHPG FEGSACQCER TTEGCLNPRR VECSGRGRCR CNVCECHSGY QLPLCQECPG CPSPCGKYIS CAECLKFEKG PFGKNCSAAC PGLQLSNNPV KGRTCKERDS EGCWVAYTLE QQDGMDRYLI YVDESRECVA GPNIAAIVGG TVAGIVLIGI LLLVIWKALI HLSDLREYRR FEKEKLKSQW NNDNPLFKSA TTTVMNPKFA ES (469-769 aa encoded by BC005861)

Target Information

CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Regulation of CD18 stability by SIGIRR-modulated ubiquitination: new insights into the relationship between innate immune response and acute lung injury.

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Bioinformatics Analysis Identified the Hub Genes, mRNA-miRNA-lncRNA Axis, and Signaling Pathways Involved in Rheumatoid Arthritis Pathogenesis.

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Mon May 24 00:00:00 EDT 2021

Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury.

International journal of molecular sciences

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Mon May 24 00:00:00 EDT 2021

Induced Pluripotent Stem Cell-Derived Conditioned Medium Promotes Endogenous Leukemia Inhibitory Factor to Attenuate Endotoxin-Induced Acute Lung Injury.

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Journal of immunology (Baltimore, Md. : 1950)

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Journal of immunology (Baltimore, Md. : 1950)

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Bioinformatics

Protein Aliases: CD18; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin beta chain, beta 2; Integrin beta-2; integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta

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Gene Aliases: 2E6; AI528527; CD18; ITGB2; LAD; LCAMB; LFA-1; Lfa1; MAC-1; MF17; MFI7

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UniProt ID: (Human) P05107, (Mouse) P11835

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Entrez Gene ID: (Human) 3689, (Mouse) 16414

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Function(s)

glycoprotein binding protein binding protein kinase binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding receptor activity integrin

Process(es)

leukocyte migration involved in inflammatory response phagocytosis apoptotic process inflammatory response cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway cell-cell signaling aging regulation of cell shape natural killer cell activation extracellular matrix organization neutrophil chemotaxis receptor internalization heterotypic cell-cell adhesion toll-like receptor 4 signaling pathway endodermal cell differentiation receptor clustering endothelial cell migration cellular extravasation positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis regulation of peptidyl-tyrosine phosphorylation regulation of immune response leukocyte migration positive regulation of NF-kappaB transcription factor activity cellular response to low-density lipoprotein particle stimulus signal transduction single organismal cell-cell adhesion activated T cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD18 Polyclonal Antibody

CD18 Antibody (10554-1-AP) in IHC (P)

Product Details

10554-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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