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CD18 Antibody (MA5-44128) in Flow
Separation of bovine leukocytes stained using anti-bovine CD18 (IVA35) Alexa Fluor® 488 Monoclonal antibody (Product # MA5-44128) concentration in sample 3 µg/mL (red-filled) from bovine leukocytes stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 488 antibody (concentration in sample 3 µg/mL, black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood.
Product Details
MA5-44128
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
IVA35
Immunogen
Bovine leukocytes
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Size-exclusion chromatography
Storage buffer
PBS
Contains
15mM sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913060
Product Specific Information
This antibody reacts with an extracellular epitope of CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.
Target Information
CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD18; CD18 subunit; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin beta chain, beta 2; Integrin beta-2; integrin, beta 2 (antigen CD18 subunit (p95), lymphocyte function-associated antigen 1, integrin B2); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); Leu-CAM receptor; leukocyte cell adhesion molecule CD18; leukocyte surface protein; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C
Gene Aliases: CD18; ITGB2; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7
UniProt ID: (Bovine) P32592, (Human) P05107
Entrez Gene ID: (Bovine) 281877, (Human) 3689
phagocytosis
receptor internalization
cellular response to low-density lipoprotein particle stimulus
positive regulation of leukocyte adhesion to vascular endothelial cell
leukocyte migration involved in inflammatory response
apoptotic process
inflammatory response
cell adhesion
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
cell-cell signaling
aging
regulation of cell shape
natural killer cell activation
extracellular matrix organization
neutrophil chemotaxis
heterotypic cell-cell adhesion
toll-like receptor 4 signaling pathway
endodermal cell differentiation
receptor clustering
endothelial cell migration
cellular extravasation
positive regulation of nitric oxide biosynthetic process
positive regulation of angiogenesis
regulation of peptidyl-tyrosine phosphorylation
regulation of immune response
leukocyte migration
positive regulation of NF-kappaB transcription factor activity
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