Invitrogen

CD18 Monoclonal Antibody (M18/2)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (90) CD18 antibodies

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FIGURE: 1 / 4

CD18 Antibody (MA1-10122) in Flow

Separation of murine myeloid cells stained using anti-mouse CD18 (M18/2) purified Monoclonal antibody (Product # MA1-10122) (concentration in sample 16 µg/mL, DAR APC, red-filled) from murine myeloid cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension.

CD18 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD18 Antibody — Fig. 3. Inhibition of VEGF-induced vascular leakage by anti-beta2 integrin antibody. A , levels of fibronectin in the retina measured by ELISA. Anti-VEGF, VEGF plus anti-VEGF antibody ( n = 4). B , levels of beta2 integrin in the retina measured by Western blotting analysis ( n = 3). C , qualitative assessment of retinal vascular integrity demonstrated by the leakage of FITC-dextran after treatments of PBS ( control ), VEGF ( VEGF ), and VEGF plus anti-beta2 integrin antibody ( anti -beta 2 integrin ). D , quantitative analysis of relative fluorescence intensity after treatments of VEGF ( VEGF ) and VEGF plus anti-beta2 integrin antibody ( anti -beta 2 integrin ) ( n = 4). E , levels of phosphorylated FAK normalized by the total amount of FAK ( p-FAK/total FAK ) in confluent HRMECs on FN1-coated dish at 5 min after treatments of VEGF ( VEGF ) and VEGF plus anti-beta2 integrin antibody ( Anti -beta 2 integrin ) ( n = 4). F , actin cytoskeleton arrangement after treatments of PBS ( Control ), VEGF ( VEGF ), and VEGF plus anti-beta2 integrin antibody ( Anti -beta 2 integrin ) demonstrated by immunofluorescence staining of F-actin ( green ). Nucleus was identified with DAPI staining. NS , p > 0.05; *, p < 0.05; **, p < 0.01 using Kruskal-Wallis test with post hoc Dunn's multiple comparison test. {TM}

Product Details

MA1-10122

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Flow Cytometry (Flow)

1 µg/mL

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

M18/2

Immunogen

Murine cytotoxic T cell glycoproteins

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152311

Product Specific Information

This antibody recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.

This antibody will not cross-react with canine.

Target Information

CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative Proteomics Reveals β2 Integrin-mediated Cytoskeletal Rearrangement in Vascular Endothelial Growth Factor (VEGF)-induced Retinal Vascular Hyperpermeability.

Molecular & cellular proteomics : MCP

Jo DH,Bae J,Chae S,Kim JH,Han JH,Hwang D,Lee SW,Kim JH

MA1-10122 was used in immunohistochemistry to elucidate the revelation of Beta2 integrin-mediated cytoskeletal rearrangement in vascular endothelial growth factor (VEGF)- induced retinal vascular hyperpermeability by quantitative proteomics

Sun May 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: CD18; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; Integrin beta-2; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta

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Gene Aliases: 2E6; AI528527; Cd18; Itgb2; LAD; LCAMB; Lfa1; MF17

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UniProt ID: (Mouse) P11835

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Entrez Gene ID: (Mouse) 16414

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Function(s)

glycoprotein binding receptor activity protein binding protein kinase binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

Process(es)

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion signal transduction integrin-mediated signaling pathway single organismal cell-cell adhesion natural killer cell activation neutrophil chemotaxis heterotypic cell-cell adhesion receptor clustering endothelial cell migration cellular extravasation positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis regulation of peptidyl-tyrosine phosphorylation activated T cell proliferation positive regulation of NF-kappaB transcription factor activity

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CD18 Monoclonal Antibody (M18/2)

CD18 Antibody (MA1-10122) in Flow

Product Details

MA1-10122

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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