Invitrogen

CD18 (LFA-1 beta) Monoclonal Antibody (M18/2), PE, eBioscience™

CD18 (LFA-1 beta) Antibody in Flow Cytometry (Flow)

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CD18 (LFA-1 beta) Antibody (12-0181-82) in Flow

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.5 µg of Anti-Mouse CD18 PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-0181-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M18/2

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465572

Product Specific Information

Description: The M18/2 monoclonal antibody reacts with mouse CD18, the 90-95 kDa integrin beta 2 glycoprotein. CD18 is the common beta subunit that non-covalently associates with CD11a (alpha L), CD11b (alpha M) and CD11c (alpha X) to form LFA-1, Mac-1 and gp150/95. Heterodimers of CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression. Through the interactions of LFA-1, Mac-1 and gp150/95 with their counter-receptors, CD18 is involved in cellular adhesion and the immune response.

Applications Reported: The M18/2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The M18/2 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Protein kinase D3 promotes neutrophil migration during viral infection.

Immunology and cell biology

Veazey JM,Wong GS,Eliseeva SI,Smyth TR,Chapman TJ,Lim K,Kim M,Georas SN

Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 12-0181-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Protein kinase D3 promotes neutrophil migration during viral infection.

Immunology and cell biology

Veazey JM,Wong GS,Eliseeva SI,Smyth TR,Chapman TJ,Lim K,Kim M,Georas SN

Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 12-0181-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Central Nervous System Barriers Impact Distribution and Expression of iNOS and Arginase-1 in Infiltrating Macrophages During Neuroinflammation.

Frontiers in immunology

Ivan DC,Walthert S,Locatelli G

Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 12-0181-82) in Flow Cytometry

Mon Oct 25 00:00:00 EDT 2021

Macrophage migration inhibitory factor promotes the migration of dendritic cells through CD74 and the activation of the Src/PI3K/myosin II pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ives A,Le Roy D,Théroude C,Bernhagen J,Roger T,Calandra T

12-0181-82 was used in Flow Cytometry to investigate whether migration inhibitory factor promoted the migration of Dendritic cells.

Sat May 01 00:00:00 EDT 2021

Type V Collagen in Scar Tissue Regulates the Size of Scar after Heart Injury.

Cell

Yokota T,McCourt J,Ma F,Ren S,Li S,Kim TH,Kurmangaliyev YZ,Nasiri R,Ahadian S,Nguyen T,Tan XHM,Zhou Y,Wu R,Rodriguez A,Cohn W,Wang Y,Whitelegge J,Ryazantsev S,Khademhosseini A,Teitell MA,Chiou PY,Birk DE,Rowat AC,Crosbie RH,Pellegrini M,Seldin M,Lusis AJ,Deb A

12-0181-82 was used in Flow Cytometry to demonstrate that collagen V regulates scar size in an integrin-dependent manner.

Thu Aug 06 00:00:00 EDT 2020

STAT6 Mediates Footpad Immunopathology in the Absence of IL-12p40 Following Infection of Susceptible BALB/c Mice With Leishmania major.

Frontiers in immunology

Kauffmann F,Meert E,de Jonge K,Elkrim Y,Hanot Mambres D,Denis O,Muraille E,Magez S,De Trez C

12-0181 was used in Flow cytometry/Cell sorting to refine the role of IL-12p40 cytokine.

Fri May 10 00:00:00 EDT 2019

Modulation of Endoplasmic Reticulum Stress Controls CD4⁺ T-cell Activation and Antitumor Function.

Cancer immunology research

Thaxton JE,Wallace C,Riesenberg B,Zhang Y,Paulos CM,Beeson CC,Liu B,Li Z

12-0181 was used in Flow cytometry/Cell sorting to investigate whether the endoplasmic reticulum stress response may be important for CD4+ T-cell activation and whether gp96 is integral to this process.

Tue Aug 01 00:00:00 EDT 2017

Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion.

Skeletal muscle

Penton CM,Badarinarayana V,Prisco J,Powers E,Pincus M,Allen RE,August PR

12-0181 was used in Flow cytometry/Cell sorting to demonstrate laminin 521 is a superior substrate for both short-term and long-term myogenic cell culture applications compared to other commonly utilized substrates.

Tue Dec 13 00:00:00 EST 2016

P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.

Cell research

Wang H,Hong LJ,Huang JY,Jiang Q,Tao RR,Tan C,Lu NN,Wang CK,Ahmed MM,Lu YM,Liu ZR,Shi WX,Lai EY,Wilcox CS,Han F

12-0181 was used in Flow cytometry/Cell sorting to demonstrate the role of the P2RX7 pathway in linking neurovascular inflammation to brain damage in vivo.

Mon Jun 01 00:00:00 EDT 2015

Kinetics of neutrophils in mice exposed to radiation and/or granulocyte colony-stimulating factor treatment.

Radiation research

Romero-Weaver AL,Wan XS,Diffenderfer ES,Lin L,Kennedy AR

12-0181 was used in Flow cytometry/Cell sorting to evaluate the involvement of SPE-like protons and γ radiation on neutropenia observed in astronauts following radiation exposure using a mouse model system.

Thu Aug 01 00:00:00 EDT 2013

Impairment of the hematological response and interleukin-1β production in protein-energy malnourished mice after endotoxemia with lipopolysaccharide.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Fock RA,Vinolo MA,Blatt SL,Borelli P

Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 12-0181-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: CD18; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; Integrin beta-2; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta

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Gene Aliases: 2E6; AI528527; Cd18; Itgb2; LAD; LCAMB; Lfa1; MF17

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UniProt ID: (Mouse) P11835

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Entrez Gene ID: (Mouse) 16414

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Function(s)

glycoprotein binding receptor activity protein binding protein kinase binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

Process(es)

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion signal transduction integrin-mediated signaling pathway single organismal cell-cell adhesion natural killer cell activation neutrophil chemotaxis heterotypic cell-cell adhesion receptor clustering endothelial cell migration cellular extravasation positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis regulation of peptidyl-tyrosine phosphorylation activated T cell proliferation positive regulation of NF-kappaB transcription factor activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD18 (LFA-1 beta) Monoclonal Antibody (M18/2), PE, eBioscience™

CD18 (LFA-1 beta) Antibody (12-0181-82) in Flow

Product Details

12-0181-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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