Invitrogen

CD19 Monoclonal Antibody (SJ25C1), PerCP-Cyanine5.5, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

7 Published Figures

17 References

View all (322) CD19 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 9

CD19 Antibody (45-0198-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0037-42) and Mouse IgG1 K Isotype Control PerCP-Cyanine5-5 (Product # 45-4714-82) (left) or Anti-Human CD19 PerCP-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD19 Antibody — Antibody clone (SJ25C1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CD19 KO cells (blue histogram) compared to the control Cas9 cells (pink histogram) using CD19 antibody (SJ25C1). Yellow histogram represents staining with the isotype control. {KO}

Product Details

45-0198-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.1 µg)/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SJ25C1

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1518739

Product Specific Information

Description: The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19, along with CD21, CD81, Leu13, and MHC class II, form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.

Applications Reported: This SJ25C1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SJ25C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Tyrosine Kinase Inhibitors Target B Lymphocytes.

Biomolecules

Upfold NLE,Petakh P,Kamyshnyi A,Oksenych V

Published figure using CD19 monoclonal antibody (Product # 45-0198-42) in Flow Cytometry

Sat Feb 25 00:00:00 EST 2023

Evaluation of peripheral lymphocyte subsets in acne vulgaris patients before and after systemic isotretinoin treatment.

Indian journal of pharmacology

Gunaydin SD,Tezcan I

Published figure using CD19 monoclonal antibody (Product # 45-0198-42) in Flow Cytometry

Wed Dec 21 00:00:00 EST 2022

Microbial byproducts determine reproductive fitness of free-living and parasitic nematodes.

Cell host & microbe

Venzon M,Das R,Luciano DJ,Burnett J,Park HS,Devlin JC,Kool ET,Belasco JG,Hubbard EJA,Cadwell K

45-0198-42 was used in Flow Cytometry to indicate that microbial byproducts mediate evolutionarily conserved transkingdom interactions that impact the reproductive fitness of distantly related nematodes.

Wed Jun 08 00:00:00 EDT 2022

Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells.

Journal of translational medicine

Park JS,Lee D,Yang S,Jeong HY,Na HS,Cho KH,Choi J,Koo H,Cho ML,Park SH

45-0198-42 was used in Flow Cytometry to study the efficacy of methotrexate-loaded nanoparticles against experimental model of rheumatoid arthritis.

Fri Feb 11 00:00:00 EST 2022

Persistence of functional memory B cells recognizing SARS-CoV-2 variants despite loss of specific IgG.

iScience

Winklmeier S,Eisenhut K,Taskin D,Rübsamen H,Gerhards R,Schneider C,Wratil PR,Stern M,Eichhorn P,Keppler OT,Klein M,Mader S,Kümpfel T,Meinl E

45-0198-42 was used in Flow Cytometry to conclude that differentiation of memory B cells could be more sensitive for detecting previous infection with COVID-19 than measuring serum antibodies.

Fri Jan 21 00:00:00 EST 2022

Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.

Nature communications

Asano Y,Daccache J,Jain D,Ko K,Kinloch A,Veselits M,Wolfgeher D,Chang A,Josephson M,Cunningham P,Tambur A,Khan AA,Pillai S,Chong AS,Clark MR

Published figure using CD19 monoclonal antibody (Product # 45-0198-42) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD19 monoclonal antibody (Product # 45-0198-42) in Flow Cytometry

Tue Apr 28 00:00:00 EDT 2020

Human C. difficile toxin-specific memory B cell repertoires encode poorly neutralizing antibodies.

JCI insight

Shah HB,Smith K,Scott EJ,Larabee JL,James JA,Ballard JD,Lang ML

Published figure using CD19 monoclonal antibody (Product # 45-0198-42) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues.

Nature immunology

Cella M,Gamini R,Sécca C,Collins PL,Zhao S,Peng V,Robinette ML,Schettini J,Zaitsev K,Gordon W,Bando JK,Yomogida K,Cortez V,Fronick C,Fulton R,Lin LL,Gilfillan S,Flavell RA,Shan L,Artyomov MN,Bowman M,Oltz EM,Jelinsky SA,Colonna M

45-0198 was used in Flow cytometry/Cell sorting to demonstrate that innate lymphoid cells (ILCs) undergo conversion from ILC3s into ILC1-like cells in human tissues in vivo, requiring tissue factors and Aiolos in the process.

Thu Aug 01 00:00:00 EDT 2019

Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

Huang A,Shinde PV,Huang J,Senff T,Xu HC,Margotta C,Häussinger D,Willnow TE,Zhang J,Pandyra AA,Timm J,Weggen S,Lang KS,Lang PA

45-0198 was used in Flow cytometry/Cell sorting to show that progranulin (PGRN) can reduce NK cell cytotoxicity through reduction of NK cell expansion, granzyme B transcription, and NK cell-mediated lysis of target cells.

Thu Sep 05 00:00:00 EDT 2019

Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation.

Journal of immunology (Baltimore, Md. : 1950)

Dai J,Luftig MA

Published figure using CD19 monoclonal antibody (Product # 45-0198-42) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets.

Scandinavian journal of immunology

Albayati Z,Alyami A,Alomar S,Middleton D,Bonnett L,Aleem S,Flanagan BF,Christmas SE

Published figure using CD19 monoclonal antibody (Product # 45-0198-42) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function.

eLife

Portugal S,Tipton CM,Sohn H,Kone Y,Wang J,Li S,Skinner J,Virtaneva K,Sturdevant DE,Porcella SF,Doumbo OK,Doumbo S,Kayentao K,Ongoiba A,Traore B,Sanz I,Pierce SK,Crompton PD

45-0198 was used in Flow cytometry/Cell sorting to elucidate the function of atypical memory B cells and their relationship to classical memory B cells.

Fri May 08 00:00:00 EDT 2015

Exposure-dependent control of malaria-induced inflammation in children.

PLoS pathogens

Portugal S,Moebius J,Skinner J,Doumbo S,Doumtabe D,Kone Y,Dia S,Kanakabandi K,Sturdevant DE,Virtaneva K,Porcella SF,Li S,Doumbo OK,Kayentao K,Ongoiba A,Traore B,Crompton PD

45-0198 was used in Flow cytometry/Cell sorting to suggest that children in malaria endemic areas can acquire exposure-dependent P. falciparum-specific immunoregulatory responses, which enhances anti-parasite effector mechanisms.

Tue Apr 01 00:00:00 EDT 2014

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

45-0198 was used in Flow cytometry/Cell sorting to expose long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

Fri Nov 01 00:00:00 EDT 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

45-0198 was used in Flow cytometry/Cell sorting to study B and T cell exhaustion caused by chronic exposure to Plasmodium falciparum.

Fri Feb 01 00:00:00 EST 2013

B cell analysis of ethnic groups in Mali with differential susceptibility to malaria.

Malaria journal

Portugal S,Doumtabe D,Traore B,Miller LH,Troye-Blomberg M,Doumbo OK,Dolo A,Pierce SK,Crompton PD

45-0198 was used in Flow cytometry/Cell sorting to determine the mechanisms underlying the elevated resistance to malaria observed in the Fulani ethnic group.

Fri May 11 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19; Differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12

View more View less

Gene Aliases: B4; CD19; CVID3

View more View less

UniProt ID: (Human) P15391

View more View less

Entrez Gene ID: (Human) 930

View more View less

Function(s)

receptor signaling protein activity protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity

Process(es)

cellular defense response cell surface receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of immune response B cell receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD19 Monoclonal Antibody (SJ25C1), PerCP-Cyanine5.5, eBioscience™

CD19 Antibody (45-0198-42) in Flow

Product Details

45-0198-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!