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Invitrogen
CD193 (CCR3) Monoclonal Antibody (eBio5E8-G9-B4 (5E8-G9-B4)), Alexa Fluor™ 700, eBioscience™
FIGURE: 1 / 1
CD193 (CCR3) Antibody (56-1939-42) in Flow
Normal human peripheral blood cells were stained with CD16 Monoclonal Antibody, eFluor 450 (Product # 48-0168-42) and Mouse IgG2b kappa Isotype Control, Alexa Fluor 700 (Product # 56-4732-80) (left) or CD193 (CCR3) Monoclonal Antibody, Alexa Fluor 700 (right). Cells in the granulocyte gate were used for analysis.
Product Details
56-1939-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio5E8-G9-B4 (5E8-G9-B4)
Conjugate
Excitation/Emission Max
696/719 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815250
Product Specific Information
Description: The eBio5E8-G9-B4 monoclonal antibody reacts with human CD193 (CCR3, CKR3). CD193 is a member of the seven transmembrane G-protein coupled receptor (GPCR) family, and is a high affinity chemokine receptor for the chemokines eotaxin-1 (CCL11), eotaxin-2 (CCL24), eotaxin-3 (CCL26) and MCP-4 (CCL13), but has also been reported to bind RANTES, MCP-3 and MCP-4. CD193 is highly expressed on the surface of eosinophils, and is the key eosinophil chemokine receptor responsible for the regulation of eosinophil migration and function. In addition to eosinophils, CD193 is also expressed on CD4+ TH2 cells, basophils, mast cells, mononuclear phagocytes, platelets, CD34+ hematopoietic progenitors and airway epithelial cells. CD193 likely plays an important role in the migration of eosinophils in allergic airway inflammation and asthma. CD193 can also serve as a receptor for HIV-1 cell entry.
Applications Reported: This eBio5E8-G9-B4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio5E8-G9-B4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm). Most instruments will require a 685 LP mirror and 710/20 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
Target Information
CCR3 (C-C chemokine receptor type 3) binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES, and MIP-1 delta. CCR3 trasnduces a signal by increasing the intracellular calcium ions level. It also serves as an alternative co-receptor with CD4 for HIV-1 infection. Other chemokines play important roles in inflammation and are critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors. The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: b-chemokine receptor; C C CKR3; C-C chemokine receptor type 3; C-C CKR-3; CC chemokine receptor 3; CCR-3; CD193; chemokine (C-C motif) receptor 3; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841
Gene Aliases: CC-CKR-3; CCR3; CD193; CKR3; CMKBR3
UniProt ID: (Human) P51677
Entrez Gene ID: (Human) 1232
positive regulation of endothelial cell proliferation
chemotaxis
inflammatory response
cellular defense response
cell adhesion
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
viral process
positive regulation of angiogenesis
chemokine-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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