Invitrogen

CD196 (CCR6) Monoclonal Antibody (R6H1), eBioscience™

CD196 (CCR6) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 14

CD196 (CCR6) Antibody (14-1969-82) in Flow

Normal human peripheral blood cells cells were stained with CD19 Monoclonal Antibody, APC (Product # 17-0199-42) and 0.125 µg of Mouse IgG1 kappa Isotype Control (Product # 14-4714-85) (left) or 0.125 µg of CD196 Monoclonal Antibody (right) followed by IgG1 Monoclonal Antibody, PE (Product # 12-4015-82). Cells in the lymphocyte gate were used for analysis.

CD196 (CCR6) Antibody in Western Blot (WB)

CD196 (CCR6) Antibody in Immunocytochemistry (ICC/IF)

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Product Details

14-1969-82

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-Dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

R6H1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1257199

Product Specific Information

Description: This R6H1 monoclonal antibody reacts with CD196 (also known as CCR6), a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, natural killer, and Langerhans cells. This CC chemokine receptor uniquely binds MIP-3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.

Applications Reported: This R6H1 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunoprecipitation.

Applications Tested: This R6H1 antibody has been tested by western blot on lysates prepared from normal human peripheral blood cells. This antibody can be used at less than or equal to 5 µg/mL.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Common transcriptional programs and the role of chemokine (C-C motif) ligand 20 (CCL20) in cell migration of cholangiocarcinoma.

EXCLI journal

Win Maung HM,Chan-On W,Kunkeaw N,Khaenam P

14-1969-82 was used in Western Blot to analyze multiple microarray datasets to identify the common transcriptional networks in CCA and select a possible biomarker for functional study in CCA cell lines.

Mon Sep 28 00:00:00 EDT 2020

Common transcriptional programs and the role of chemokine (C-C motif) ligand 20 (CCL20) in cell migration of cholangiocarcinoma.

EXCLI journal

Win Maung HM,Chan-On W,Kunkeaw N,Khaenam P

Mon Sep 28 00:00:00 EDT 2020

Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.

Genes

Zou M,Zhang X,Jiang D,Zhao Y,Wu T,Gong Q,Su H,Wu D,Moreland L,Li G

Published figure using CD196 (CCR6) monoclonal antibody (Product # 14-1969-82) in Western Blot

Mon Dec 21 00:00:00 EST 2020

The Calcineurin Inhibitor Tacrolimus Specifically Suppresses Human T Follicular Helper Cells.

Frontiers in immunology

Wallin EF,Hill DL,Linterman MA,Wood KJ

14-1969 was used in Flow cytometry/Cell sorting to study the impact of the immunosuppressive agent Tacrolimuson T follicular helper cells differentiationand function in transplant recipients.

Fri Aug 16 00:00:00 EDT 2019

The Rheumatoid Arthritis Risk Variant CCR6DNP Regulates CCR6 via PARP-1.

PLoS genetics

Li G,Cunin P,Wu D,Diogo D,Yang Y,Okada Y,Plenge RM,Nigrovic PA

14-1969 was used in Western Blotting to investigate whether CCR6DNP is causal for rheumatoid arthritis.

Thu Sep 01 00:00:00 EDT 2016

Expression of CCL20 and Its Corresponding Receptor CCR6 Is Enhanced in Active Inflammatory Bowel Disease, and TLR3 Mediates CCL20 Expression in Colonic Epithelial Cells.

PloS one

Skovdahl HK,Granlund Av,Østvik AE,Bruland T,Bakke I,Torp SH,Damås JK,Sandvik AK

Tue Jun 14 00:00:00 EDT 2016

CCR6 is a prognostic marker for overall survival in patients with colorectal cancer, and its overexpression enhances metastasis in vivo.

PloS one

Liu J,Ke F,Xu Z,Liu Z,Zhang L,Yan S,Wang Z,Wang H,Wang H

Published figure using CD196 (CCR6) monoclonal antibody (Product # 14-1969-82) in Western Blot

Thu Feb 12 00:00:00 EST 2015

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD196 (CCR6) monoclonal antibody (Product # 14-1969-82) in Flow Cytometry

Fri Oct 13 00:00:00 EDT 2017

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD196 (CCR6) monoclonal antibody (Product # 14-1969-82) in Flow Cytometry

Fri Oct 13 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: C-C chemokine receptor type 6; C-C CKR-6; CD196; chemokine (C-C motif) receptor 6; chemokine (C-C) receptor 6; Chemokine receptor-like 3; CKR-L3; CKR6; DRY-6; DRY6; G protein-coupled receptor 29; G-protein coupled receptor 29; GPR-CY4; GPRCY4; LARC receptor; seven-transmembrane receptor, lymphocyte, 22

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Gene Aliases: BN-1; C-C CKR-6; CC-CKR-6; CCR-6; CCR6; CD196; CKR-L3; CKRL3; CMKBR6; DCR2; DRY6; GPR29; GPRCY4; STRL22

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UniProt ID: (Human) P51684

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Entrez Gene ID: (Human) 1235

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Function(s)

receptor activity chemokine receptor activity protein binding C-C chemokine receptor activity C-C chemokine binding

Process(es)

dendritic cell chemotaxis leukocyte migration involved in inflammatory response movement of cell or subcellular component chemotaxis immune response humoral immune response cellular defense response signal transduction G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration positive regulation of epithelial cell migration calcium-mediated signaling isotype switching to IgA isotypes cell chemotaxis positive regulation of sperm motility involved in capacitation chemokine-mediated signaling pathway lymphocyte migration T cell migration thymocyte migration DN2 thymocyte differentiation DN3 thymocyte differentiation regulation of T cell migration positive regulation of dendritic cell chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD196 (CCR6) Monoclonal Antibody (R6H1), eBioscience™

CD196 (CCR6) Antibody (14-1969-82) in Flow

Product Details

14-1969-82

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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