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Invitrogen
CD196 (CCR6) Monoclonal Antibody (sirx6), eFluor™ 660, eBioscience™
FIGURE: 1 / 2
CD196 (CCR6) Antibody (50-7196-82) in Flow
Staining of BALB/c splenocytes with Anti-Mouse CD19 PE (Product # 12-0193-82) and 0.5 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.5 µg of Anti-Mouse CD196 (CCR6) eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
50-7196-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
sirx6
Immunogen
N-terminal extracellular domain of mouse CCR6
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11219682
Product Specific Information
Description: The sirx6 monoclonal antibody reacts with mouse CD196 (CCR6). CD196 is a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, NK and Langerhans cells. This CC chemokine receptor uniquely binds MIP3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.
Due to expression characteristics of some chemokine receptors (shedding, surface cleavage or intracellular cycling), we recommend a fixation step after surface staining. Refer to Applications Tested for more information.
Applications Reported: This sirx6 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This sirx6 antibody has been tested by flow cytometric analysis of mouse splenocytes. Cells are surface stained, then washed with Flow Cytometry Staining Buffer, immediately fixed with IC Fixation Buffer for 20-30 minutes, and then washed with Flow Cytometry Staining Buffer prior to analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C chemokine receptor type 6; C-C CKR-6; CD196; CKR6; DRY-6; KY411
Gene Aliases: CC-CKR-6; CCR-6; Ccr6; Cmkbr6; KY411
UniProt ID: (Mouse) O54689
Entrez Gene ID: (Mouse) 12458
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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