Invitrogen

CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), PE, eBioscience™

CD199 (CCR9) Antibody in Flow Cytometry (Flow)

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CD199 (CCR9) Antibody (12-1991-82) in Flow

Staining of BALB/c thymocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82), Anti-Mouse CD8a APC (Product # 17-0081-82) and 0.25 µg of Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (left) or 0.25 µg of Anti-Mouse CD199 (CCR9) PE (right). Samples were gated on CD4 single positive (orange histogram) or CD4+CD8+ double positive cells (purple histogram).

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Product Details

12-1991-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2a

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioCW-1.2 (CW-1.2)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1257161

Product Specific Information

Description: The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CD199), which is the receptor for thymus-expressed chemokine (TECK). CCR9 is a member of the G protein coupled receptor (GPCR) supergene family, and is involved in trafficking of T cell progenitors within the thymus. CCR9 expression during thymocyte development commences at the double-negative (DN) 3 stage (CD4-CD8-CD25+CD44-), peaks in the double-positive (DP) stage (CD4+CD8+CD25-CD44-), and is down-regulated in commited CD4+ or CD8+ single-positive (SP) thymoyctes. CCR9-deficient mice show a mild impairment in thymoycte development. In the periphery, CCR9 is thought to be expressed on naive CD8+ T cells, but not on naive CD4+ T cells.

Applications Reported: This eBioCW-1.2 (CW-1.2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioCW-1.2 (CW-1.2) antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Krüppel-like factor 2 controls IgA plasma cell compartmentalization and IgA responses.

Mucosal immunology

Wittner J,Schulz SR,Steinmetz TD,Berges J,Hauke M,Channell WM,Cunningham AF,Hauser AE,Hutloff A,Mielenz D,Jäck HM,Schuh W

12-1991-82 was used in Flow cytometry/Cell sorting to conclude that KLF2 not only orchestrates the localization of IgA plasma cells by fine-tuning chemokine receptors and adhesion molecules but also controls IgA responses to Salmonella flagellin.

Fri Apr 01 00:00:00 EDT 2022

Contact-dependent delivery of IL-2 by dendritic cells to CD4 T cells in the contraction phase promotes their long-term survival.

Protein & cell

Tong D,Zhang L,Ning F,Xu Y,Hu X,Shi Y

Published figure using CD199 (CCR9) monoclonal antibody (Product # 12-1991-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Foxp3(+) T cells expressing RORγt represent a stable regulatory T-cell effector lineage with enhanced suppressive capacity during intestinal inflammation.

Mucosal immunology

Yang BH,Hagemann S,Mamareli P,Lauer U,Hoffmann U,Beckstette M,Föhse L,Prinz I,Pezoldt J,Suerbaum S,Sparwasser T,Hamann A,Floess S,Huehn J,Lochner M

12-1991 was used in Flow cytometry/Cell sorting to demonstrate that Foxp3(+) T cells expressing retinoic acid-related orphan receptor-γt, represent a stable regulatory T cell lineage with a highly suppressive phenotype in vivo.

Tue Mar 01 00:00:00 EST 2016

Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.

Nature communications

Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY

Published figure using CD199 (CCR9) monoclonal antibody (Product # 12-1991-82) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2016

Vitamin A Impairs the Reprogramming of Tregs into IL-17-Producing Cells during Intestinal Inflammation.

BioMed research international

Tejón G,Manríquez V,De Calisto J,Flores-Santibáñez F,Hidalgo Y,Crisóstomo N,Fernández D,Sauma D,Mora JR,Bono MR,Rosemblatt M

12-1991 was used in Flow cytometry/Cell sorting to study the contribution of different factors to the reprogramming of iTregs into IL-17-producing cells, showing that vitamin A impairs reprogramming during intestinal inflammation.

Fri Sep 02 00:00:00 EDT 2016

The thymic medulla is required for Foxp3+ regulatory but not conventional CD4+ thymocyte development.

The Journal of experimental medicine

Cowan JE,Parnell SM,Nakamura K,Caamano JH,Lane PJ,Jenkinson EJ,Jenkinson WE,Anderson G

12-1991 was used in Flow cytometry/Cell sorting to demonstrate a differential requirement for the thymic medulla in relation to CD4 conventional and Foxp3(+) thymocyte lineages.

Mon Apr 08 00:00:00 EDT 2013

Therapeutic evaluation of ex vivo-generated versus natural regulatory T-cells in a mouse model of chronic gut inflammation.

Inflammatory bowel diseases

Karlsson F,Martinez NE,Gray L,Zhang S,Tsunoda I,Grisham MB

12-1991 was used in Flow cytometry/Cell sorting to study the effect of ex vivo-generated and isolated Treg cells on a mouse model of chronic colitis, showing that ex-vivo Tregs were more potent at attenuating colitis, likely due to the associated decrease in IL-6 and IL-17A expression in the colon.

Tue Oct 01 00:00:00 EDT 2013

Plasmacytoid dendritic cells play a key role in promoting atherosclerosis in apolipoprotein E-deficient mice.

Arteriosclerosis, thrombosis, and vascular biology

Macritchie N,Grassia G,Sabir SR,Maddaluno M,Welsh P,Sattar N,Ialenti A,Kurowska-Stolarska M,McInnes IB,Brewer JM,Garside P,Maffia P

12-1991 was used in Flow cytometry/Cell sorting to identify a potential role for pDC targeting in the control of the pathology of coronary artery disease.

Thu Nov 01 00:00:00 EDT 2012

Vitamin A deficiency impairs vaccine-elicited gastrointestinal immunity.

Journal of immunology (Baltimore, Md. : 1950)

Kaufman DR,De Calisto J,Simmons NL,Cruz AN,Villablanca EJ,Mora JR,Barouch DH

12-1991 was used in Flow cytometry/Cell sorting to suggest that oral vitamin A supplementation may be important for optimizing the success of vaccines against HIV-1 and other mucosal pathogens in the developing world.

Mon Aug 15 00:00:00 EDT 2011

Clonal analysis reveals uniformity in the molecular profile and lineage potential of CCR9(+) and CCR9(-) thymus-settling progenitors.

Journal of immunology (Baltimore, Md. : 1950)

Desanti GE,Jenkinson WE,Parnell SM,Boudil A,Gautreau-Rolland L,Eksteen B,Ezine S,Lane PJ,Jenkinson EJ,Anderson G

12-1991 was used in Flow cytometry/Cell sorting to suggest that the earliest T cell progenitors represent a developmentally homogeneous progenitor pool that ensures the efficient generation of the first cohorts of T cells during thymus development.

Sun May 01 00:00:00 EDT 2011

CD22 expression mediates the regulatory functions of peritoneal B-1a cells during the remission phase of contact hypersensitivity reactions.

Journal of immunology (Baltimore, Md. : 1950)

Nakashima H,Hamaguchi Y,Watanabe R,Ishiura N,Kuwano Y,Okochi H,Takahashi Y,Tamaki K,Sato S,Tedder TF,Fujimoto M

12-1991 was used in Flow cytometry/Cell sorting to clarify the roles of B cells in contact hypersensitivity (CHS), showing that CD22 expression mediates the regulatory functions of peritoneal B-1a cells during the remission phase of CHS.

Sat May 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR-9; CDw199; CDw199 antigen; Chemokine C-C receptor 10

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Gene Aliases: A130091K22Rik; Ccr9; Cmkbr10; GPR-9-6

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UniProt ID: (Mouse) Q9WUT7

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Entrez Gene ID: (Mouse) 12769

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Function(s)

signal transducer activity G-protein coupled receptor activity chemokine receptor activity protein binding C-C chemokine receptor activity

Process(es)

chemotaxis immune response signal transduction G-protein coupled receptor signaling pathway chemokine-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), PE, eBioscience™

CD199 (CCR9) Antibody (12-1991-82) in Flow

Product Details

12-1991-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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