Invitrogen

CD1d Monoclonal Antibody (1B1), APC, eBioscience™

FIGURE: 1 / 4

CD1d Antibody (17-0011-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.125 µg of Anti-Mouse CD1d APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-0011-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1B1

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573135

Product Specific Information

Description: The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with beta2m, functionally CD1d is similar to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes. 1B1 detects beta2m associated CD1d.

Applications Reported: This 1B1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1B1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cissus quadrangularis (Hadjod) Inhibits RANKL-Induced Osteoclastogenesis and Augments Bone Health in an Estrogen-Deficient Preclinical Model of Osteoporosis Via Modulating the Host Osteoimmune System.

Cells

Azam Z,Sapra L,Baghel K,Sinha N,Gupta RK,Soni V,Saini C,Mishra PK,Srivastava RK

17-0011-82 was used in Flow cytometry/Cell sorting to demonstrate that, in a dose-dependent manner CQ, suppresses the RANKL-induced osteoclastogenesis (p 0.001) as well as inhibiting the osteoclast functional activity (p 0.001) in mouse bone marrow cells (BMCs).

Wed Jan 04 00:00:00 EST 2023

Bifidobacterium longum Ameliorates Ovariectomy-Induced Bone Loss via Enhancing Anti-Osteoclastogenic and Immunomodulatory Potential of Regulatory B Cells (Bregs).

Frontiers in immunology

Sapra L,Shokeen N,Porwal K,Saini C,Bhardwaj A,Mathew M,Mishra PK,Chattopadhyay N,Dar HY,Verma B,Srivastava RK

17-0011-82 was used in Enzyme-linked immunosorbent assay to establish a novel osteo-protective and immunomodulatory potential of BL in augmenting bone health under osteoporotic conditions.

Tue Jun 14 00:00:00 EDT 2022

Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells.

Journal of translational medicine

Park JS,Lee D,Yang S,Jeong HY,Na HS,Cho KH,Choi J,Koo H,Cho ML,Park SH

Published figure using CD1d monoclonal antibody (Product # 17-0011-82) in Flow Cytometry

Fri Feb 11 00:00:00 EST 2022

Transplantation tolerance modifies donor-specific B cell fate to suppress de novo alloreactive B cells.

The Journal of clinical investigation

Khiew SH,Jain D,Chen J,Yang J,Yin D,Young JS,Dent A,Sciammas R,Alegre ML,Chong AS

17-0011 was used in Flow cytometry/Cell sorting to showed that alloreactive B cells were not deleted but rapidly lost their ability to differentiate into germinal center B cells and secrete donor-specific antibodies., by using a murine model of heart tolerance.

Wed Jul 01 00:00:00 EDT 2020

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using CD1d monoclonal antibody (Product # 17-0011-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

Development of Hematopoietic Stem Cell-Engineered Invariant Natural Killer T Cell Therapy for Cancer.

Cell stem cell

Zhu Y,Smith DJ,Zhou Y,Li YR,Yu J,Lee D,Wang YC,Di Biase S,Wang X,Hardoy C,Ku J,Tsao T,Lin LJ,Pham AT,Moon H,McLaughlin J,Cheng D,Hollis RP,Campo-Fernandez B,Urbinati F,Wei L,Pang L,Rezek V,Berent-Maoz B,Macabali MH,Gjertson D,Wang X,Galic Z,Kitchen SG,An DS,Hu-Lieskovan S,Kaplan-Lefko PJ,De Oliveira SN,Seet CS,Larson SM,Forman SJ,Heath JR,Zack JA,Crooks GM,Radu CG,Ribas A,Kohn DB,Witte ON,Yang L

17-0011 was used in Flow cytometry/Cell sorting to develop a proof-of-concept for hematopoietic stem cell-engineered iNKT (HSC-iNKT) cell therapy with the potential to provide therapeutic levels of iNKT cells for a patient's lifetime.

Thu Oct 03 00:00:00 EDT 2019

Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.

PloS one

Komegae EN,Souza TA,Grund LZ,Lima C,Lopes-Ferreira M

17001182 was used in flow cytometry to study the therapeutic TnP peptide in inflammation and demyelination in a mouse model of multiple sclerosis.

Thu Aug 17 00:00:00 EDT 2017

Natural killer T cell sensitization during neonatal respiratory syncytial virus infection induces eosinophilic lung disease in re-infected adult mice.

PloS one

Lee SY,Noh Y,Goo JH,Rho S,Kim MJ,Kang CY,Song M,Kim JO

Thu Sep 21 00:00:00 EDT 2017

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using CD1d monoclonal antibody (Product # 17-0011-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD1d monoclonal antibody (Product # 17-0011-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Bioinformatics

Protein Aliases: Antigen-presenting glycoprotein CD1d1; CD1d.1; T-cell surface glycoprotein CD1d1

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Gene Aliases: AI747460; CD1.1; Cd1a; Cd1d; Cd1d1; Ly-38

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UniProt ID: (Mouse) P11609

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Entrez Gene ID: (Mouse) 12479

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Function(s)

protein binding beta-2-microglobulin binding lipid antigen binding endogenous lipid antigen binding exogenous lipid antigen binding histone binding T cell receptor binding cell adhesion molecule binding lipopeptide binding major histocompatibility complex protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD1d Monoclonal Antibody (1B1), APC, eBioscience™

CD1d Antibody (17-0011-82) in Flow

Product Details

17-0011-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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