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Invitrogen
CD2 Monoclonal Antibody (RPA-2.10), Biotin, eBioscience™
FIGURE: 1 / 2
CD2 Antibody (13-0029-82) in Flow
Staining of normal human peripheral blood cells with 0.125 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.125 µg of Anti-Human CD2 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
Product Details
13-0029-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Baboon,
Chimpanzee,
Cynomolgus monkey,
Human,
Non-human primate,
Pig,
Rhesus monkey
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
RPA-2.10
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_466314
Product Specific Information
Description: The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.
RPA-2.10 crossreacts to non-human primates and pigs.
Applications Reported: The RPA-2.10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The RPA-2.10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. The interaction between CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection. Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD2; CD2 antigen; CD2 antigen (p50), sheep red blood cell receptor; cluster of differentiation 2; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; Rosette receptor; surface immunoglobulin superfamily molecule; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5
Gene Aliases: CD2; LFA-2; SRBC; T11
UniProt ID: (Human) P06729
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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