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Product Details
MA1-82679
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rhesus monkey
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
2H7
Immunogen
Proprietary Immunogen.
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_927855
Product Specific Information
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human CD20 antibody, clone 2H7 recognizes the human CD20 cell surface antigen, a 33-37 kDa non-glycosylated phosphoprotein.
Target Information
CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APY; ATOPY; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MGC3969
Gene Aliases: B1; Bp35; CD20; CVID5; EGK_06167; LEU-16; MS4A1; MS4A2; S7
UniProt ID: (Human) P11836
Entrez Gene ID: (Human) 931, (Rhesus monkey) 696843
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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