Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
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Description: The monoclonal antibody OX90.1 recognizes mouse CD200 also known as OX2. CD200 is a member of the Ig superfamily with 2 Ig domains, a transmembrane and cytoplasmic domain. CD200 is expressed on resting and activated B cells, a subset of resting and activated T cells, keratinocytes, peripheral and central nerve cells, follicular dendritic cells and ovarian cells. The interaction with CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF alpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes.
Applications Reported: This OX90 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OX90 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.
Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
CD200 is a type-1 membrane glycoprotein that contains two immunoglobulin domains, and belongs to the immunoglobulin superfamily. CD200 is a broadly distributed cell surface glycoprotein that interacts with a receptor on myeloid cells (CD200R) and is implicated in locally preventing macrophage activation. Distant, but recognizable, homologues of CD200 have been identified in many herpesviruses and poxviruses. Studies of the related genes in mouse and rat suggest that CD200 may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants that encode different isoforms of CD200 have been found. Diseases associated with CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Protein Aliases: antigen identified by monoclonal antibody MRC OX-2; MRC OX-2 antigen; OX-2 membrane glycoprotein
Gene Aliases: Mox2; OX2
Entrez Gene ID: (Mouse) 17470
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